##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550349_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695391
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25499035794251	33.0	31.0	34.0	30.0	34.0
2	32.365965334610316	34.0	31.0	34.0	30.0	34.0
3	32.46152020949365	34.0	31.0	34.0	30.0	34.0
4	35.95920999840378	37.0	35.0	37.0	35.0	37.0
5	35.92161532145225	37.0	35.0	37.0	35.0	37.0
6	35.93638974332426	37.0	35.0	37.0	35.0	37.0
7	36.1224836099403	37.0	35.0	37.0	35.0	37.0
8	36.11782579872331	37.0	35.0	37.0	35.0	37.0
9	37.71410760277312	39.0	38.0	39.0	35.0	39.0
10	37.42039802068189	39.0	37.0	39.0	35.0	39.0
11	37.339670775146644	39.0	37.0	39.0	34.0	39.0
12	37.19447620115877	39.0	37.0	39.0	34.0	39.0
13	37.13391027493885	39.0	37.0	39.0	33.0	39.0
14	38.30498956702057	40.0	38.0	41.0	34.0	41.0
15	38.36900678898634	40.0	38.0	41.0	34.0	41.0
16	38.395075576186635	40.0	37.0	41.0	34.0	41.0
17	38.36422818241824	40.0	37.0	41.0	34.0	41.0
18	38.33667965216691	40.0	37.0	41.0	34.0	41.0
19	38.282495746997014	40.0	37.0	41.0	34.0	41.0
20	38.1905920554048	40.0	37.0	41.0	34.0	41.0
21	38.1330675835609	40.0	37.0	41.0	34.0	41.0
22	38.11581541895135	40.0	37.0	41.0	34.0	41.0
23	38.07160719652685	40.0	36.0	41.0	34.0	41.0
24	38.04960806222686	40.0	36.0	41.0	34.0	41.0
25	38.01727804932764	40.0	36.0	41.0	34.0	41.0
26	37.89653159157941	40.0	36.0	41.0	33.0	41.0
27	37.82882867336506	40.0	36.0	41.0	33.0	41.0
28	37.72953777083684	40.0	36.0	41.0	33.0	41.0
29	37.66100654164348	40.0	36.0	41.0	33.0	41.0
30	37.61863038204406	40.0	36.0	41.0	33.0	41.0
31	37.49234603266364	40.0	35.0	41.0	33.0	41.0
32	37.29060053984018	39.0	35.0	41.0	32.0	41.0
33	37.20174693086336	39.0	35.0	41.0	32.0	41.0
34	36.969791095944586	39.0	35.0	41.0	31.0	41.0
35	36.93042043972384	39.0	35.0	41.0	31.0	41.0
36	36.80949566502874	39.0	35.0	41.0	31.0	41.0
37	36.757132318364775	39.0	35.0	41.0	31.0	41.0
38	36.619582364453954	39.0	35.0	41.0	31.0	41.0
39	36.52652536486667	39.0	35.0	41.0	31.0	41.0
40	36.43518826099274	39.0	35.0	41.0	30.0	41.0
41	36.324871906596435	39.0	35.0	41.0	30.0	41.0
42	36.29254764585679	39.0	35.0	41.0	30.0	41.0
43	36.2087099200306	38.0	35.0	41.0	30.0	41.0
44	36.00068882110928	38.0	35.0	40.0	30.0	41.0
45	35.87884801500163	38.0	35.0	40.0	29.0	41.0
46	35.72358716175504	38.0	35.0	40.0	29.0	41.0
47	35.60062037041032	38.0	35.0	40.0	29.0	41.0
48	35.5046211411997	37.0	34.0	40.0	28.0	41.0
49	35.41169356520289	37.0	34.0	40.0	28.0	41.0
50	35.29041647073373	37.0	34.0	40.0	28.0	41.0
51	34.03764500834782	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	6.0
12	8.0
13	3.0
14	13.0
15	25.0
16	39.0
17	80.0
18	152.0
19	353.0
20	504.0
21	818.0
22	1193.0
23	1885.0
24	2796.0
25	4084.0
26	5547.0
27	6856.0
28	8128.0
29	9360.0
30	11337.0
31	14141.0
32	18103.0
33	24685.0
34	44824.0
35	57622.0
36	54811.0
37	80478.0
38	138734.0
39	208647.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.13330054602375	20.38795440263104	23.52662027549968	11.952124775845531
2	33.32341085806402	23.385979973856436	24.20681314541028	19.08379602266926
3	32.999851881890905	24.55366836786786	25.074238809533057	17.372240940708178
4	28.457802876367396	26.203387734382527	25.503062305954494	19.835747083295587
5	25.91117802790085	30.316757047474013	23.879227657533676	19.892837267091465
6	27.538895384035744	33.46822147540017	23.182065916872666	15.81081722369142
7	82.13350474768872	5.620147514132337	7.569122982609784	4.677224755569169
8	82.96239094264953	4.317427174064663	7.987448787804271	4.732733095481534
9	76.68635343281693	6.566521568441351	10.37790250377126	6.3692224949704555
10	41.065673843923776	26.645872609797944	16.716926160965556	15.571527385312722
11	32.163919291448984	25.288506753754366	23.220030170076978	19.327543784719676
12	27.94571687007741	23.014678073199107	26.988269908583806	22.05133514813968
13	25.268805607205152	24.70983950036742	28.37396515054121	21.64738974188622
14	21.57922665090575	25.991995869949424	29.454076915001774	22.97470056414305
15	21.572324059414054	24.423957169419793	31.698425777727923	22.305292993438226
16	25.079128145173	24.287055771501212	29.364055617630946	21.26976046569484
17	22.925663403754147	24.56804876680889	28.170051093557436	24.336236735879528
18	26.422976426212013	22.856493684847806	27.805507980402393	22.915021908537785
19	24.751542657296397	26.433330313449556	25.384711622669837	23.43041540658421
20	28.524959339421997	25.23486786570433	25.485086807278208	20.755085987595468
21	25.738038024650876	27.321319948057997	25.651180415047076	21.289461612244047
22	25.624145265037946	23.144964487604817	26.01155321250922	25.21933703484802
23	23.945521296651812	26.955051187030033	26.33151708894708	22.767910427371078
24	24.32200014092791	23.384398129972922	28.536176050595998	23.75742567850317
25	24.261171053407363	25.41850556018125	25.663259950157535	24.65706343625385
26	23.72463836891763	25.73760661268265	26.19245863118735	24.34529638721237
27	24.887437427289107	24.696465729352262	25.675051877289178	24.74104496606945
28	21.855617918552298	27.57685963724006	26.189582551399145	24.377939892808506
29	23.706231458273116	25.81741782680535	25.190144824997734	25.286205889923796
30	26.5683622595058	23.061989585715086	26.976334177462753	23.39331397731636
31	27.018468746359964	25.191295256913016	24.498016223966086	23.292219772760937
32	25.436193450878715	25.0608650385179	25.81051523531366	23.69242627528973
33	25.7215005658687	25.226671038307945	24.355650274449914	24.696178121373443
34	23.533666670980786	25.839851249153355	28.295735780302017	22.33074629956384
35	23.26820450652942	25.92814689865126	27.462679269648298	23.340969325171017
36	24.5906259931463	29.661873679699625	24.397353431378892	21.350146895775183
37	24.99759128317738	27.197936125143983	25.54548448283052	22.258988108848115
38	23.685092271829806	28.501231681169298	25.795559620414988	22.018116426585905
39	25.177633877919043	25.204525223938763	25.4177865402342	24.200054357907995
40	25.52952224000598	23.599385094141283	28.03545055946942	22.835642106383318
41	21.587279674312725	25.539013303307062	27.229716806803655	25.64399021557656
42	24.68970694184998	25.025345453133564	26.04304628619007	24.241901318826386
43	24.857526197491772	24.64771617694218	26.282048516589946	24.212709108976103
44	24.619242987038948	24.538568948979783	25.43906953066692	25.403118533314352
45	24.677771210728928	22.66983610659327	26.75617026967562	25.896222413002178
46	24.74262680995296	23.675313600549906	26.232867552211637	25.3491920372855
47	22.53063384484412	25.362709612290065	28.60218208173531	23.5044744611305
48	23.774106941274763	24.300141934537546	28.237351360601448	23.68839976358624
49	25.08818779650585	24.30675691805042	26.822463908793758	23.782591376649968
50	22.20750628063924	23.905975199563986	28.578885835450844	25.307632684345926
51	22.630721421473673	22.8228435513258	26.663416696505994	27.883018330694533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	573.5
2	894.0
3	2403.0
4	3912.0
5	2659.0
6	1406.0
7	1389.0
8	1372.0
9	1452.5
10	1533.0
11	1545.5
12	1558.0
13	1575.0
14	1592.0
15	1559.0
16	1526.0
17	1469.0
18	1412.0
19	1478.0
20	1544.0
21	1595.5
22	1647.0
23	1856.5
24	2066.0
25	2154.5
26	2884.0
27	3525.0
28	4360.0
29	5195.0
30	6099.5
31	7004.0
32	8245.5
33	9487.0
34	11358.0
35	13229.0
36	14219.5
37	15210.0
38	17455.5
39	19701.0
40	21374.0
41	23047.0
42	26255.5
43	29464.0
44	33018.5
45	36573.0
46	43231.5
47	49890.0
48	56078.5
49	62267.0
50	65179.0
51	68091.0
52	63595.0
53	59099.0
54	55268.5
55	51438.0
56	47731.0
57	44024.0
58	41421.5
59	38819.0
60	37018.0
61	35217.0
62	32188.5
63	29160.0
64	26286.5
65	23413.0
66	19658.0
67	15903.0
68	13383.5
69	10864.0
70	9077.5
71	7291.0
72	6268.5
73	5246.0
74	4705.0
75	3362.0
76	2560.0
77	1935.0
78	1310.0
79	946.5
80	583.0
81	459.5
82	336.0
83	269.0
84	202.0
85	127.5
86	53.0
87	41.5
88	30.0
89	24.0
90	18.0
91	13.5
92	9.0
93	8.0
94	7.0
95	4.0
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.828541641560854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38821242643814	23.763994459994777
2	9.23743924784277	6.0650331801839
3	3.2875214017215	3.2377359970181034
4	1.8151282556336232	2.383520540993685
5	1.2500883658928643	2.0519288987672333
6	1.0142715244837317	1.997823298641816
7	0.878371950837774	2.018496911539984
8	0.8084976248419464	2.1233438355381504
9	0.7841820436231208	2.3169197586281327
>10	8.40817293128642	48.7368325002836
>50	0.08540948495413352	1.857201778532514
>100	0.03962295690678613	2.4020773183000492
>500	0.0017610203069682725	0.39181898976603086
>1k	0.0013207652302262044	0.653272531812047
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2167	0.31162324505206423	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1201	0.17270859128173935	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	1152	0.1656621958006359	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	989	0.1422221455267612	Illumina Paired End PCR Primer 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15804058436189136	0.0
2	0.0	0.0	0.0	0.6459675204309518	0.0
3	0.0	0.0	0.0	0.9561527255889133	0.0
4	0.0	0.0	0.0	1.3278860382144722	0.0
5	0.0	0.0	0.0	2.377798964898884	0.0
6	0.0	0.0	0.0	3.2413419213075807	0.0
7	0.0	0.0	0.0	4.02708691944532	0.0
8	0.0	0.0	0.0	5.347926562178688	0.0
9	0.0	0.0	0.0	5.919403616095118	0.0
10	0.0	0.0	0.0	6.971617406610094	0.0
11	0.0	0.0	0.0	8.21221442325253	0.0
12	0.0	0.0	0.0	9.152117298038082	0.0
13	0.0	0.0	0.0	9.576626674777211	0.0
14	0.0	0.0	0.0	9.778096063941005	0.0
15	0.0	0.0	0.0	10.114022183203407	0.0
16	1.4380398941027422E-4	0.0	0.0	10.721306430482995	0.0
17	1.4380398941027422E-4	0.0	0.0	11.426521194550979	0.0
18	1.4380398941027422E-4	0.0	0.0	12.163948052246866	0.0
19	1.4380398941027422E-4	0.0	0.0	12.635193725544333	0.0
20	1.4380398941027422E-4	0.0	0.0	13.090045744049032	0.0
21	1.4380398941027422E-4	0.0	0.0	13.665117897700718	0.0
22	1.4380398941027422E-4	0.0	0.0	14.26420531758392	0.0
23	1.4380398941027422E-4	0.0	0.0	14.8881708276351	0.0
24	1.4380398941027422E-4	0.0	0.0	15.387745886846393	0.0
25	1.4380398941027422E-4	0.0	0.0	15.844035945245192	0.0
26	1.4380398941027422E-4	0.0	0.0	16.258622846715014	0.0
27	2.8760797882054843E-4	0.0	0.0	16.684714067337655	0.0
28	2.8760797882054843E-4	0.0	0.0	17.105053128383886	0.0
29	2.8760797882054843E-4	0.0	0.0	17.556310047153328	0.0
30	2.8760797882054843E-4	0.0	0.0	18.120884509578065	0.0
31	2.8760797882054843E-4	0.0	0.0	18.582063903616813	0.0
32	4.3141196823082265E-4	0.0	0.0	19.073442135431723	0.0
33	4.3141196823082265E-4	0.0	0.0	19.53591576537516	0.0
34	7.190199470513711E-4	0.0	0.0	19.967040125627165	0.0
35	8.628239364616453E-4	0.0	0.0	20.448495882172764	0.0
36	8.628239364616453E-4	0.0	0.0	20.91312657195736	0.0
37	8.628239364616453E-4	0.0	0.0	21.37387455402788	0.0
38	8.628239364616453E-4	0.0	0.0	21.864677569885142	0.0
39	8.628239364616453E-4	0.0	0.0	22.39632091873493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	30	2.164259E-6	45.000004	1
TCACGAC	40	6.8084773E-9	45.0	25
TACGAAT	50	2.1827873E-11	45.0	12
TATAGCG	40	6.8084773E-9	45.0	1
CGCCCAT	20	7.031363E-4	45.0	19
TACGTCG	20	7.031363E-4	45.0	1
TAGTACG	20	7.031363E-4	45.0	1
GCGAAAG	70	0.0	41.785713	1
TAGGGCG	210	0.0	41.785713	5
GCTACGA	55	6.002665E-11	40.90909	10
AACGGGC	160	0.0	39.375	4
AAACACG	720	0.0	39.0625	40
CACGACC	755	0.0	39.039734	27
GACACGA	755	0.0	38.741722	25
CGTTTTT	1235	0.0	38.623478	1
AACACGT	705	0.0	38.617023	41
CGGTCTA	35	6.244729E-6	38.571426	31
ATAACGG	35	6.244729E-6	38.571426	2
ACGACCA	760	0.0	38.486843	28
GGCGATA	170	0.0	38.382355	8
>>END_MODULE