##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550348_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	775892
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27640960339841	33.0	31.0	34.0	30.0	34.0
2	32.411066230867185	34.0	31.0	34.0	30.0	34.0
3	32.44941177380357	34.0	31.0	34.0	30.0	34.0
4	35.980543683914775	37.0	35.0	37.0	35.0	37.0
5	35.96122914013806	37.0	35.0	37.0	35.0	37.0
6	35.98055270578895	37.0	35.0	37.0	35.0	37.0
7	36.16091285900615	37.0	35.0	37.0	35.0	37.0
8	36.158737298489996	37.0	36.0	37.0	35.0	37.0
9	37.76241796538694	39.0	38.0	39.0	35.0	39.0
10	37.50376727688905	39.0	37.0	39.0	35.0	39.0
11	37.45357730199564	39.0	37.0	39.0	35.0	39.0
12	37.3955769617421	39.0	37.0	39.0	35.0	39.0
13	37.35701231614709	39.0	37.0	39.0	34.0	39.0
14	38.583282209379654	40.0	38.0	41.0	34.0	41.0
15	38.64545323318194	40.0	38.0	41.0	35.0	41.0
16	38.64835827666737	40.0	38.0	41.0	35.0	41.0
17	38.65124398756528	40.0	38.0	41.0	35.0	41.0
18	38.55001855928403	40.0	38.0	41.0	35.0	41.0
19	38.459679955457716	40.0	37.0	41.0	34.0	41.0
20	38.29576539002851	40.0	37.0	41.0	34.0	41.0
21	38.25283157965284	40.0	37.0	41.0	34.0	41.0
22	38.228928768436845	40.0	37.0	41.0	34.0	41.0
23	38.192530661483815	40.0	37.0	41.0	34.0	41.0
24	38.1807674263944	40.0	37.0	41.0	34.0	41.0
25	38.141404989354186	40.0	36.0	41.0	34.0	41.0
26	38.03206889618658	40.0	36.0	41.0	34.0	41.0
27	37.97862460239312	40.0	36.0	41.0	34.0	41.0
28	37.88531651312296	40.0	36.0	41.0	34.0	41.0
29	37.81597181050971	40.0	36.0	41.0	33.0	41.0
30	37.749731921452984	40.0	36.0	41.0	33.0	41.0
31	37.610597866713405	40.0	36.0	41.0	33.0	41.0
32	37.364716996695414	40.0	36.0	41.0	33.0	41.0
33	37.14114979919886	40.0	35.0	41.0	32.0	41.0
34	36.87758863346961	40.0	35.0	41.0	31.0	41.0
35	36.7932663308811	40.0	35.0	41.0	31.0	41.0
36	36.620970444340195	39.0	35.0	41.0	30.0	41.0
37	36.57743345723374	39.0	35.0	41.0	30.0	41.0
38	36.46421924700861	39.0	35.0	41.0	30.0	41.0
39	36.3736241641878	39.0	35.0	41.0	30.0	41.0
40	36.30363504199038	39.0	35.0	41.0	30.0	41.0
41	36.2100227866765	39.0	35.0	41.0	30.0	41.0
42	36.159560866718564	39.0	35.0	41.0	30.0	41.0
43	36.108026632572574	39.0	35.0	41.0	29.0	41.0
44	35.9226992932006	39.0	35.0	41.0	29.0	41.0
45	35.86053084707665	39.0	35.0	41.0	28.0	41.0
46	35.75817897336227	39.0	35.0	41.0	28.0	41.0
47	35.66933542297124	38.0	35.0	40.0	28.0	41.0
48	35.56157042474984	38.0	35.0	40.0	27.0	41.0
49	35.4847839647786	38.0	35.0	40.0	27.0	41.0
50	35.404494439947825	38.0	35.0	40.0	27.0	41.0
51	34.19185917627711	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	7.0
13	8.0
14	13.0
15	22.0
16	45.0
17	84.0
18	148.0
19	313.0
20	549.0
21	859.0
22	1466.0
23	2374.0
24	4004.0
25	6449.0
26	9014.0
27	10332.0
28	10446.0
29	10747.0
30	11829.0
31	14467.0
32	17739.0
33	23925.0
34	41922.0
35	54536.0
36	56339.0
37	82309.0
38	147201.0
39	268530.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86804864594557	20.44666010217917	24.229016409500293	15.45627484237497
2	35.71334670289164	23.08078959442809	23.822387651889695	17.383476050790573
3	30.984982446010527	23.20090940491718	29.578472261603416	16.235635887468874
4	27.384352461425042	25.190748196914004	29.007387626112912	18.41751171554804
5	24.88052460909508	29.109463688245274	27.119496012331613	18.890515690328037
6	25.82343934465106	33.558020961680235	26.062905662128234	14.555634031540471
7	79.3811509849309	5.089368107932548	10.992767034587288	4.536713872549273
8	80.04116552303671	4.633247926257778	10.951524181200476	4.374062369505034
9	73.30594979713672	7.120191985482515	13.305846690003248	6.268011527377522
10	36.14523670820166	30.141050558582894	19.46559572724039	14.248117005975057
11	26.429967057270858	24.4026230454754	29.875034154237962	19.29237574301578
12	24.58770035004872	22.39564269253968	32.5603305614699	20.456326395941705
13	23.226557304367102	23.24576100797534	33.92856222257737	19.599119465080193
14	20.29238605372913	26.106855077768554	32.9032906641646	20.697468204337717
15	20.141334103199927	25.318214390662618	34.42089878488243	20.119552721255022
16	22.57943115794466	25.04150062122048	32.226650100787225	20.152418120047635
17	22.11171142375485	24.884648894433763	30.542266191686473	22.461373490124913
18	22.493465585416526	24.465647280807122	31.759059250514248	21.281827883262103
19	22.361875106329233	26.56542405386317	29.54960226423265	21.52309857557495
20	23.30543426146938	26.381635588458185	29.364653843576168	20.948276306496265
21	22.67918730957401	26.559237625855143	30.646146628654503	20.11542843591634
22	22.200383558536497	24.163543379748727	29.489542358988107	24.14653070272667
23	20.810499399400946	25.976166786099096	30.35770442278049	22.855629391719464
24	21.30077381903667	24.891608625942784	31.52500605754409	22.282611497476452
25	22.069179731199704	26.372871482113492	29.190531671933723	22.367417114753085
26	20.925206085383017	26.835822511380446	30.030468157939506	22.208503245297027
27	22.137874858872113	25.042789460388814	30.46996231434272	22.349373366396357
28	20.521799425693267	26.045248565522005	30.611992390693548	22.820959618091177
29	21.61976151320029	24.07164914704624	30.91899388059163	23.389595459161843
30	22.712310476200294	23.798415243358612	32.061807571156805	21.427466709284282
31	23.13015213457543	25.38626509875086	29.52034561511138	21.963237151562332
32	23.212637841349054	25.26098993158842	29.833404649100647	21.69296757796188
33	22.40917550380723	25.221809220870945	29.74151041639816	22.627504858923665
34	21.600944461342557	25.720074443350367	30.4355503085481	22.24343078675898
35	21.48997540894867	25.905022864006845	30.225727291942693	22.37927443510179
36	22.04288741216561	27.362571079480137	29.229454614817524	21.36508689353673
37	22.70290195027143	27.70089136116882	28.626535651869073	20.969671036690674
38	21.905110505070294	27.01458450402891	28.25225675738376	22.828048233517038
39	22.523366654121965	26.28909693617153	28.423672366772696	22.76386404293381
40	21.99971130002629	25.721878818186035	30.132157568321364	22.14625231346631
41	20.295737035566805	26.534620797739894	29.202904527949762	23.96673763874354
42	21.60571316626541	26.796899568496645	28.817541616616744	22.7798456486212
43	22.350146669897356	25.062766467498054	29.74782572832301	22.839261134281575
44	22.722492305630166	25.065215261917896	28.829398936965454	23.382893495486485
45	22.489599067911513	23.589236646337376	29.428838034159394	24.492326251591717
46	22.46562665938043	24.72637944456187	29.142844622705223	23.665149273352476
47	20.947245235161592	25.455604646007433	30.300351079789458	23.296799039041517
48	21.36508689353673	24.529702587473516	29.993994009475543	24.11121650951421
49	21.98128089991906	24.295778278420194	30.470477850010052	23.252462971650694
50	20.925850504967187	23.67378449578034	31.11502631809582	24.285338681156656
51	21.087084284926252	23.383537915070654	29.046954988580886	26.482422811422207
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	603.5
2	904.0
3	5510.5
4	10117.0
5	6864.5
6	3612.0
7	3577.5
8	3543.0
9	3543.0
10	3543.0
11	3600.5
12	3658.0
13	3611.5
14	3565.0
15	3453.0
16	3341.0
17	3181.5
18	3022.0
19	2988.0
20	2954.0
21	3052.5
22	3151.0
23	3236.5
24	3322.0
25	3846.5
26	5059.5
27	5748.0
28	6739.5
29	7731.0
30	9205.5
31	10680.0
32	12195.0
33	13710.0
34	15492.5
35	17275.0
36	19659.5
37	22044.0
38	24651.5
39	27259.0
40	31332.0
41	35405.0
42	40865.5
43	46326.0
44	55620.5
45	64915.0
46	72000.5
47	79086.0
48	79355.5
49	79625.0
50	70533.0
51	61441.0
52	55897.0
53	50353.0
54	45679.0
55	41005.0
56	38554.5
57	36104.0
58	33084.5
59	30065.0
60	27875.5
61	25686.0
62	23438.0
63	21190.0
64	18728.0
65	16266.0
66	13844.0
67	11422.0
68	9358.0
69	7294.0
70	6375.0
71	5456.0
72	4609.5
73	3763.0
74	3339.5
75	2289.0
76	1662.0
77	1305.5
78	949.0
79	770.0
80	591.0
81	448.0
82	305.0
83	193.5
84	82.0
85	70.0
86	58.0
87	46.0
88	34.0
89	23.5
90	13.0
91	12.0
92	11.0
93	9.0
94	7.0
95	7.5
96	8.0
97	4.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	775892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.59902438606753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5611716021729	26.922671133356246
2	9.243226408317785	6.765861374479321
3	3.404217783545824	3.73773149026435
4	1.9620198759616143	2.8723205314507325
5	1.3888399701923615	2.541509396870777
6	1.100281176375749	2.4161530563424263
7	0.9394294533898558	2.40675410315238
8	0.8307672729555873	2.432421734563869
9	0.7391538117393726	2.4347077542813658
>10	6.7342147037728575	41.631774158562415
>50	0.06374370098777368	1.5935393713597028
>100	0.028684661157559056	1.7491992538151786
>500	0.0014165264769163274	0.3603122351515884
>1k	0.002478921334603573	1.3244714859762883
>5k	3.5413161922908184E-4	0.8105729203733935
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6254	0.8060400158784986	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	1868	0.24075515664551253	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	1735	0.22361359570661896	No Hit
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1581	0.2037654725142159	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	1290	0.16626025271558414	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1264	0.1629092708779057	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCT	1258	0.16213596737690297	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	1223	0.15762503028772046	No Hit
CGTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	877	0.11303119506323046	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2888391683378616E-4	0.0	0.0	0.1832729297376439	0.0
2	1.2888391683378616E-4	0.0	0.0	0.6833425270527341	0.0
3	1.2888391683378616E-4	0.0	0.0	1.1790300711954758	0.0
4	1.2888391683378616E-4	0.0	0.0	1.6473942249694544	0.0
5	1.2888391683378616E-4	0.0	0.0	2.8937017007521666	0.0
6	1.2888391683378616E-4	0.0	0.0	4.100828465817408	0.0
7	1.2888391683378616E-4	0.0	0.0	5.114500471715136	0.0
8	1.2888391683378616E-4	0.0	0.0	6.610198326571224	0.0
9	1.2888391683378616E-4	0.0	0.0	7.318930985240214	0.0
10	1.2888391683378616E-4	0.0	0.0	8.458522577884551	0.0
11	1.2888391683378616E-4	0.0	0.0	9.880112180561213	0.0
12	1.2888391683378616E-4	0.0	0.0	10.97137230439288	0.0
13	1.2888391683378616E-4	0.0	0.0	11.507400514504596	0.0
14	1.2888391683378616E-4	0.0	0.0	11.781794373443727	0.0
15	1.2888391683378616E-4	0.0	0.0	12.12874987756028	0.0
16	1.2888391683378616E-4	0.0	0.0	12.77729374706789	0.0
17	1.2888391683378616E-4	0.0	0.0	13.571605326514515	0.0
18	1.2888391683378616E-4	0.0	0.0	14.397751233419084	0.0
19	1.2888391683378616E-4	0.0	0.0	14.936872657534812	0.0
20	2.577678336675723E-4	0.0	0.0	15.454212699705629	0.0
21	2.577678336675723E-4	0.0	0.0	16.09760121253989	0.0
22	2.577678336675723E-4	0.0	0.0	16.794218783026505	0.0
23	2.577678336675723E-4	0.0	0.0	17.463126311393854	0.0
24	2.577678336675723E-4	0.0	0.0	18.044908311981565	0.0
25	2.577678336675723E-4	0.0	0.0	18.546653400215494	0.0
26	2.577678336675723E-4	0.0	0.0	19.011279920401293	0.0
27	2.577678336675723E-4	0.0	0.0	19.48840818051997	0.0
28	3.8665175050135844E-4	0.0	0.0	19.98809112608456	0.0
29	5.155356673351446E-4	0.0	0.0	20.52360380052894	0.0
30	5.155356673351446E-4	0.0	0.0	21.158614858769003	0.0
31	5.155356673351446E-4	0.0	0.0	21.708691415815604	0.0
32	5.155356673351446E-4	0.0	0.0	22.249617214767003	0.0
33	5.155356673351446E-4	0.0	0.0	22.755615472256448	0.0
34	5.155356673351446E-4	0.0	0.0	23.25813386399138	0.0
35	5.155356673351446E-4	0.0	0.0	23.77689162924737	0.0
36	5.155356673351446E-4	0.0	0.0	24.282889886736815	0.0
37	5.155356673351446E-4	0.0	0.0	24.787985956808424	0.0
38	5.155356673351446E-4	0.0	0.0	25.30158836539106	0.0
39	5.155356673351446E-4	0.0	0.0	25.846122914013804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	70	0.0	45.000004	14
GGTTACG	30	2.1645847E-6	45.000004	1
TACGTTG	35	1.2112469E-7	45.000004	1
CCCCGTA	20	7.031868E-4	45.0	44
TATAGCG	55	1.8189894E-12	44.999996	1
CGTTTTT	3960	0.0	42.15909	1
TACGAAT	75	0.0	42.0	12
GCTACGA	75	0.0	42.0	10
CGTAAGG	135	0.0	41.666664	2
TAGCGCG	50	1.0804797E-9	40.5	1
CGTATGG	45	1.9268555E-8	40.0	2
GGATCGA	40	3.45628E-7	39.375	8
TAGCCGT	35	6.245664E-6	38.57143	44
GTTCGAT	35	6.245664E-6	38.57143	39
ACATACG	70	0.0	38.57143	17
GGTACGC	30	1.1395796E-4	37.500004	8
TTAAGCG	30	1.1395796E-4	37.500004	1
TCTCGCA	30	1.1395796E-4	37.500004	19
TGTAACG	30	1.1395796E-4	37.500004	1
ATAGCGG	180	0.0	37.5	2
>>END_MODULE