SRR3550346_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626160
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4093	0.6536667944295388	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1667	0.2662258847578894	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1488	0.23763894212341896	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1329	0.21224607129168263	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1298	0.20729525999744475	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	1048	0.16736936246326817	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	904	0.14437204548358248	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	833	0.13303309058387633	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGCG	35	1.2107375E-7	45.000004	1
CGCATCG	20	7.030825E-4	45.0	21
TTATACG	20	7.030825E-4	45.0	1
CATAGCG	20	7.030825E-4	45.0	1
GTGCTCG	25	3.888765E-5	44.999996	1
ACCACGC	50	2.1827873E-11	44.999996	20
CGTTTTT	2505	0.0	42.30539	1
CGTAAGG	60	3.6379788E-12	41.249996	2
TATAGCG	50	1.0804797E-9	40.499996	1
TACGTGG	40	3.4548611E-7	39.375	2
AACACGT	475	0.0	38.842106	41
GCGAGAC	535	0.0	38.69159	21
CGTTGAT	35	6.243734E-6	38.57143	25
TACGAAG	35	6.243734E-6	38.57143	1
CAATGCG	35	6.243734E-6	38.57143	1
GTACGAG	35	6.243734E-6	38.57143	1
ACGACCA	535	0.0	38.271027	28
TCAAGCG	555	0.0	38.10811	17
TACGGGA	285	0.0	37.894737	4
ACGGGAC	155	0.0	37.741936	5