##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550346_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	626160
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12923054810272	33.0	31.0	34.0	30.0	34.0
2	32.24788552446659	34.0	31.0	34.0	30.0	34.0
3	32.31597195604957	34.0	31.0	34.0	30.0	34.0
4	35.86372652357225	37.0	35.0	37.0	35.0	37.0
5	35.84737127890635	37.0	35.0	37.0	35.0	37.0
6	35.86697329755973	37.0	35.0	37.0	35.0	37.0
7	36.07408489842852	37.0	35.0	37.0	35.0	37.0
8	36.072069439120995	37.0	35.0	37.0	35.0	37.0
9	37.67384534304331	39.0	38.0	39.0	35.0	39.0
10	37.36003098249649	39.0	37.0	39.0	34.0	39.0
11	37.29401111536988	39.0	37.0	39.0	34.0	39.0
12	37.1516305736553	39.0	37.0	39.0	34.0	39.0
13	37.06454260891785	39.0	37.0	39.0	33.0	39.0
14	38.18405359652485	40.0	37.0	41.0	33.0	41.0
15	38.25663408713428	40.0	37.0	41.0	33.0	41.0
16	38.288518908905075	40.0	37.0	41.0	34.0	41.0
17	38.25921489715089	40.0	37.0	41.0	34.0	41.0
18	38.20786859588603	40.0	37.0	41.0	34.0	41.0
19	38.12726779097994	40.0	37.0	41.0	34.0	41.0
20	37.994215535965246	40.0	36.0	41.0	34.0	41.0
21	37.950939057110006	40.0	36.0	41.0	34.0	41.0
22	37.94389293471317	40.0	36.0	41.0	34.0	41.0
23	37.88993547974958	40.0	36.0	41.0	34.0	41.0
24	37.882410246582346	40.0	36.0	41.0	34.0	41.0
25	37.85016130062604	40.0	36.0	41.0	34.0	41.0
26	37.72311709467229	40.0	35.0	41.0	33.0	41.0
27	37.67062891273796	40.0	35.0	41.0	33.0	41.0
28	37.57214929091606	40.0	35.0	41.0	33.0	41.0
29	37.51128944678676	39.0	35.0	41.0	33.0	41.0
30	37.44522965376262	39.0	35.0	41.0	33.0	41.0
31	37.317425258719815	39.0	35.0	41.0	33.0	41.0
32	37.09076274434649	39.0	35.0	41.0	32.0	41.0
33	36.91357640219752	39.0	35.0	41.0	31.0	41.0
34	36.681851284016865	39.0	35.0	41.0	31.0	41.0
35	36.60900568544781	39.0	35.0	41.0	31.0	41.0
36	36.45733358885908	39.0	35.0	41.0	30.0	41.0
37	36.41493228567778	39.0	35.0	41.0	30.0	41.0
38	36.30603360163536	39.0	35.0	41.0	30.0	41.0
39	36.19448383799668	39.0	35.0	41.0	30.0	41.0
40	36.10813050977386	39.0	35.0	41.0	30.0	41.0
41	35.96402357224991	39.0	35.0	41.0	29.0	41.0
42	35.87790341126868	38.0	35.0	40.0	29.0	41.0
43	35.802261402836336	38.0	35.0	40.0	29.0	41.0
44	35.63855883480261	38.0	35.0	40.0	28.0	41.0
45	35.56046697329756	38.0	35.0	40.0	28.0	41.0
46	35.44083780503386	38.0	35.0	40.0	28.0	41.0
47	35.309344257058896	38.0	35.0	40.0	27.0	41.0
48	35.192029193816275	38.0	34.0	40.0	27.0	41.0
49	35.1093458540948	37.0	34.0	40.0	27.0	41.0
50	35.00955985690558	37.0	34.0	40.0	26.0	41.0
51	33.73218187044845	36.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	2.0
11	2.0
12	5.0
13	14.0
14	9.0
15	14.0
16	39.0
17	82.0
18	157.0
19	318.0
20	506.0
21	853.0
22	1346.0
23	2145.0
24	3419.0
25	5123.0
26	6833.0
27	7957.0
28	8427.0
29	9470.0
30	10933.0
31	13139.0
32	16953.0
33	22610.0
34	41178.0
35	51807.0
36	48793.0
37	69233.0
38	118616.0
39	186061.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43876964354158	20.641689025169285	24.061422000766576	13.85811933052255
2	34.42969847962182	23.730196754823048	23.96384310719305	17.87626165836208
3	30.886195221668583	24.128976619394404	28.313370384566245	16.67145777437077
4	27.024083301392615	25.71515267663217	28.195509135045356	19.065254886929857
5	24.989140155870704	30.26255270218475	25.97115753162131	18.777149610323242
6	25.581640475277883	33.17442826114731	25.809537498402964	15.434393765171842
7	79.99441037434522	5.149801967548231	10.04455730164814	4.811230356458413
8	80.48869298581832	4.323016481410502	10.091989267918743	5.096301264852434
9	74.08553724287722	6.3731314679954005	12.73508368468123	6.806247604446147
10	38.492398109109494	27.623291171585535	18.552925769771306	15.331384949533666
11	30.05238277756484	24.983231123035647	26.06186917081896	18.902516928580553
12	27.141465440143094	22.36281461607257	29.629807078063113	20.86591286572122
13	24.27813977258209	23.722051871726077	31.85942889996167	20.140379455730166
14	20.450204420595377	26.515587070397345	31.451865337932798	21.582343171074488
15	20.93666155615178	24.534144627571226	34.13440654145905	20.394787274817936
16	24.282611473105916	24.369330522550147	31.12958349303692	20.218474511307015
17	21.914047527788423	24.27159192538648	30.238756867254374	23.575603679570715
18	24.595151399003452	23.34610962054427	30.69039862016098	21.3683403602913
19	23.23591414334994	27.626644946978406	27.30037051232912	21.837070397342533
20	25.171681359396956	27.245592180912226	27.02072952599974	20.56199693369107
21	23.14057110003833	27.92896384310719	28.092500319407183	20.837964737447297
22	22.550785741663475	24.529992334227675	28.4075955027469	24.51162642136195
23	21.64031557429411	27.10760827903411	28.23160214641625	23.020474000255525
24	22.948128273923597	24.34984668455347	29.84540692474767	22.856618116775266
25	22.49169541331289	25.525584515139897	27.94892679187428	24.033793279672928
26	21.20145010859844	25.804107576338314	29.071004216174778	23.923438098888465
27	22.587038456624505	24.724191899833908	28.11629615433755	24.57247348920404
28	19.8342276734381	26.889453174907374	28.61441165197394	24.661907499680595
29	22.357224990417784	24.4191580426728	28.27584004088412	24.9477769260253
30	24.913440654145905	22.73220902005877	29.062859333077807	23.291490992717517
31	24.567682381499935	25.443816276989907	26.180688641880668	23.807812699629487
32	24.31327456241216	24.403826498019676	26.951897278650822	24.331001660917337
33	23.646831480771688	24.618308419573275	25.141018270090708	26.59384182956433
34	20.633863549252588	26.219656317874023	27.85438226651335	25.292097866360034
35	21.589210425450364	26.130062603807335	27.202312507985177	25.07841446275712
36	22.301967548230483	29.08841190749968	24.352881052766065	24.25673949150377
37	22.70569822409608	28.090743579915678	25.103967037178997	24.09959115880925
38	22.418870576210555	27.06688386354925	24.533346109620542	25.98089945061965
39	22.614667177718157	26.0781589370129	24.65008943401048	26.657084451258466
40	24.099431455219115	24.40845790213364	27.50239555385205	23.989715088795197
41	21.55199948894851	26.803532643413824	26.236744602018653	25.40772326561901
42	23.47690686086623	25.915740385843872	26.530120097099786	24.077232656190112
43	23.168199821131978	24.385141177973683	26.605021080873897	25.841637920020442
44	22.24495336655168	24.423150632426214	25.953749840296407	27.37814616072569
45	22.664654401430944	22.53385716110898	26.787402580810017	28.014085856650055
46	23.489842851667305	23.815159064775777	26.447553340999107	26.247444742557814
47	21.12143860993995	25.151079596269327	28.542065925642007	25.185415868148713
48	21.944710617094675	23.686597674715728	28.307141944550914	26.061549763638688
49	23.09250031940718	23.133065031301904	28.373099527277372	25.401335122013542
50	20.825507857416632	22.902772454324776	29.0136706273157	27.25804906094289
51	20.78797751373451	22.746103232400664	27.393956816149228	29.071962437715598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	538.0
2	838.0
3	3612.5
4	6387.0
5	4213.0
6	2039.0
7	2071.5
8	2104.0
9	2169.0
10	2234.0
11	2328.0
12	2422.0
13	2443.0
14	2464.0
15	2439.0
16	2414.0
17	2263.0
18	2112.0
19	2098.5
20	2085.0
21	2181.0
22	2277.0
23	2341.5
24	2406.0
25	2580.5
26	2868.5
27	2982.0
28	3784.5
29	4587.0
30	5115.0
31	5643.0
32	7333.5
33	9024.0
34	10406.0
35	11788.0
36	13405.0
37	15022.0
38	16067.5
39	17113.0
40	19579.5
41	22046.0
42	24402.5
43	26759.0
44	29940.5
45	33122.0
46	36138.0
47	39154.0
48	47599.5
49	56045.0
50	62999.5
51	69954.0
52	69844.0
53	69734.0
54	59671.5
55	49609.0
56	43004.5
57	36400.0
58	32789.5
59	29179.0
60	27182.5
61	25186.0
62	23247.5
63	21309.0
64	18723.0
65	16137.0
66	13356.5
67	10576.0
68	8767.0
69	6958.0
70	5878.5
71	4799.0
72	4184.5
73	3570.0
74	3064.0
75	2312.5
76	2067.0
77	1527.5
78	988.0
79	738.5
80	489.0
81	372.0
82	255.0
83	223.0
84	191.0
85	132.5
86	74.0
87	60.5
88	47.0
89	28.5
90	10.0
91	8.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	626160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.81531705151157
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47789857599015	28.1640534798139
2	9.97280717903149	7.542497307373329
3	3.548897088968789	4.026080058076236
4	1.877044673419261	2.839241577808014
5	1.2518010906816555	2.36686275647774
6	0.9305715246398694	2.1113914346019103
7	0.7581269027538657	2.006816643504254
8	0.6464775626866256	1.9557403199746553
9	0.5802125648726114	1.9746829888135673
>10	5.834427449180229	40.727223222544076
>50	0.0916196646804654	2.3354150129809135
>100	0.025874071954845014	1.7323133330114344
>500	0.0016966604560554107	0.4656394079381661
>1k	0.002544990684083116	1.7520424570818423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4093	0.6536667944295388	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1667	0.2662258847578894	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1488	0.23763894212341896	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	1329	0.21224607129168263	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	1298	0.20729525999744475	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTT	1048	0.16736936246326817	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	904	0.14437204548358248	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	833	0.13303309058387633	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5970359013670627E-4	0.0	0.0	0.23204931646863422	0.0
2	1.5970359013670627E-4	0.0	0.0	0.9074357991567651	0.0
3	1.5970359013670627E-4	0.0	0.0	1.4556982240960776	0.0
4	1.5970359013670627E-4	0.0	0.0	1.981282739235978	0.0
5	1.5970359013670627E-4	0.0	0.0	3.4369809633320556	0.0
6	1.5970359013670627E-4	0.0	0.0	4.692730292576977	0.0
7	1.5970359013670627E-4	0.0	0.0	5.860962054426984	0.0
8	3.1940718027341253E-4	0.0	0.0	7.698192155359653	0.0
9	3.1940718027341253E-4	0.0	0.0	8.592851667305482	0.0
10	3.1940718027341253E-4	0.0	0.0	10.068512840168648	0.0
11	3.1940718027341253E-4	0.0	0.0	11.737575060687364	0.0
12	3.1940718027341253E-4	0.0	0.0	13.037402580810017	0.0
13	3.1940718027341253E-4	0.0	0.0	13.665836207997955	0.0
14	3.1940718027341253E-4	0.0	0.0	13.96384310719305	0.0
15	4.791107704101188E-4	0.0	0.0	14.363421489715089	0.0
16	4.791107704101188E-4	0.0	0.0	15.125686725437587	0.0
17	4.791107704101188E-4	0.0	0.0	16.051967548230483	0.0
18	4.791107704101188E-4	0.0	0.0	17.001245688003067	0.0
19	4.791107704101188E-4	0.0	0.0	17.594225118180656	0.0
20	4.791107704101188E-4	0.0	0.0	18.196946467356586	0.0
21	4.791107704101188E-4	0.0	0.0	18.9140155870704	0.0
22	4.791107704101188E-4	0.0	0.0	19.678836080235083	0.0
23	4.791107704101188E-4	0.0	0.0	20.451162642136197	0.0
24	6.388143605468251E-4	0.0	0.0	21.097962182189857	0.0
25	6.388143605468251E-4	0.0	0.0	21.6776862143861	0.0
26	6.388143605468251E-4	0.0	0.0	22.225788935735274	0.0
27	6.388143605468251E-4	0.0	0.0	22.76031685192283	0.0
28	6.388143605468251E-4	0.0	0.0	23.311453941484604	0.0
29	6.388143605468251E-4	0.0	0.0	23.895010859844128	0.0
30	6.388143605468251E-4	0.0	0.0	24.592117030790853	0.0
31	6.388143605468251E-4	0.0	0.0	25.150600485498913	0.0
32	6.388143605468251E-4	0.0	0.0	25.739267918742815	0.0
33	7.985179506835314E-4	0.0	0.0	26.312923214513862	0.0
34	7.985179506835314E-4	0.0	0.0	26.867094672288232	0.0
35	7.985179506835314E-4	0.0	0.0	27.454165069630765	0.0
36	7.985179506835314E-4	0.0	0.0	28.011370895617734	0.0
37	9.582215408202376E-4	0.0	0.0	28.578638047783315	0.0
38	9.582215408202376E-4	0.0	0.0	29.18742813338444	0.0
39	9.582215408202376E-4	0.0	0.0	29.87814616072569	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGCG	35	1.2107375E-7	45.000004	1
CGCATCG	20	7.030825E-4	45.0	21
TTATACG	20	7.030825E-4	45.0	1
CATAGCG	20	7.030825E-4	45.0	1
GTGCTCG	25	3.888765E-5	44.999996	1
ACCACGC	50	2.1827873E-11	44.999996	20
CGTTTTT	2505	0.0	42.30539	1
CGTAAGG	60	3.6379788E-12	41.249996	2
TATAGCG	50	1.0804797E-9	40.499996	1
TACGTGG	40	3.4548611E-7	39.375	2
AACACGT	475	0.0	38.842106	41
GCGAGAC	535	0.0	38.69159	21
CGTTGAT	35	6.243734E-6	38.57143	25
TACGAAG	35	6.243734E-6	38.57143	1
CAATGCG	35	6.243734E-6	38.57143	1
GTACGAG	35	6.243734E-6	38.57143	1
ACGACCA	535	0.0	38.271027	28
TCAAGCG	555	0.0	38.10811	17
TACGGGA	285	0.0	37.894737	4
ACGGGAC	155	0.0	37.741936	5
>>END_MODULE