FastQCFastQC Report
Sat 18 Jun 2016
SRR3550344_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550344_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603735
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.5596826422188543No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG7240.11992016364795813TruSeq Adapter, Index 10 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC6490.10749749476177463RNA PCR Primer, Index 10 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGT207.0306234E-445.045
CCCAATC302.1637825E-644.99999644
CGTTTTT18850.040.2254641
ATAAGCG356.243361E-638.5714261
CGTAAGG659.094947E-1238.0769272
TTAAGCA950.037.89473745
CATACGA601.546141E-1037.49999618
GCGATAT301.13928014E-437.4999969
TCGATCA850.037.05882317
ACACGAC3650.036.98629826
TTAATCG552.744855E-936.8181820
CGTTGAT552.744855E-936.8181825
GGCCGAT1100.036.818188
TAATCGT552.744855E-936.8181821
CGACCAA3750.036.029
AAACACG3500.036.040
CGAGACA3750.036.022
ACGTAAG250.002106317836.01
GGACCGT250.002106317836.08
CCAACGG250.002106317836.02
Produced by FastQC (version 0.11.5)