##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550344_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603735
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3567956139697	33.0	31.0	34.0	31.0	34.0
2	32.47791166654244	34.0	31.0	34.0	31.0	34.0
3	32.54371537181048	34.0	31.0	34.0	31.0	34.0
4	36.03818728415613	37.0	35.0	37.0	35.0	37.0
5	36.003670484566904	37.0	35.0	37.0	35.0	37.0
6	36.005626640827515	37.0	35.0	37.0	35.0	37.0
7	36.15370154123912	37.0	35.0	37.0	35.0	37.0
8	36.129291825055695	37.0	36.0	37.0	35.0	37.0
9	37.70029565951949	39.0	38.0	39.0	35.0	39.0
10	37.47918374783639	39.0	37.0	39.0	35.0	39.0
11	37.42337118106454	39.0	37.0	39.0	35.0	39.0
12	37.3085310608131	39.0	37.0	39.0	34.0	39.0
13	37.26645796582938	39.0	37.0	39.0	34.0	39.0
14	38.491444093849125	40.0	38.0	41.0	34.0	41.0
15	38.5535110603162	40.0	38.0	41.0	34.0	41.0
16	38.559039976148476	40.0	38.0	41.0	34.0	41.0
17	38.567813693093825	40.0	38.0	41.0	34.0	41.0
18	38.49027636297382	40.0	38.0	41.0	34.0	41.0
19	38.40726643312049	40.0	37.0	41.0	34.0	41.0
20	38.28279957266019	40.0	37.0	41.0	34.0	41.0
21	38.222746734908526	40.0	37.0	41.0	34.0	41.0
22	38.19523300785941	40.0	37.0	41.0	34.0	41.0
23	38.15383404970724	40.0	37.0	41.0	34.0	41.0
24	38.10881761037541	40.0	36.0	41.0	34.0	41.0
25	38.06609853660961	40.0	36.0	41.0	34.0	41.0
26	37.93473957945125	40.0	36.0	41.0	34.0	41.0
27	37.8683114280272	40.0	36.0	41.0	33.0	41.0
28	37.747511739422094	40.0	36.0	41.0	33.0	41.0
29	37.672179018940426	40.0	36.0	41.0	33.0	41.0
30	37.62687271733459	40.0	36.0	41.0	33.0	41.0
31	37.47494182050072	40.0	35.0	41.0	33.0	41.0
32	37.25884866704763	40.0	35.0	41.0	32.0	41.0
33	37.115457940984044	40.0	35.0	41.0	32.0	41.0
34	36.85731488152915	39.0	35.0	41.0	31.0	41.0
35	36.81745633431886	39.0	35.0	41.0	31.0	41.0
36	36.65045425559227	39.0	35.0	41.0	31.0	41.0
37	36.58916743273125	39.0	35.0	41.0	30.0	41.0
38	36.47982310119506	39.0	35.0	41.0	30.0	41.0
39	36.390177809800655	39.0	35.0	41.0	30.0	41.0
40	36.29237330948181	39.0	35.0	41.0	30.0	41.0
41	36.13290599352365	39.0	35.0	41.0	30.0	41.0
42	36.034339569513115	39.0	35.0	41.0	29.0	41.0
43	35.95818364017325	39.0	35.0	41.0	29.0	41.0
44	35.75354915650078	38.0	35.0	41.0	28.0	41.0
45	35.68034154057658	38.0	35.0	40.0	28.0	41.0
46	35.560179548974304	38.0	35.0	40.0	27.0	41.0
47	35.4392639154596	38.0	35.0	40.0	27.0	41.0
48	35.291932718825315	38.0	34.0	40.0	27.0	41.0
49	35.16442810173338	37.0	34.0	40.0	26.0	41.0
50	35.07845660761758	37.0	34.0	40.0	26.0	41.0
51	33.80074370377732	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	0.0
10	6.0
11	6.0
12	3.0
13	7.0
14	17.0
15	32.0
16	41.0
17	99.0
18	152.0
19	301.0
20	529.0
21	857.0
22	1277.0
23	1925.0
24	2957.0
25	4292.0
26	5949.0
27	7130.0
28	8016.0
29	8897.0
30	10043.0
31	11993.0
32	14950.0
33	20185.0
34	35670.0
35	46035.0
36	45622.0
37	65997.0
38	112433.0
39	198175.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.64216088184386	19.6917521760375	22.877421385210397	13.788665556908247
2	35.35889090412184	23.252420349987993	23.390726063587504	17.997962682302663
3	32.35641465212386	23.984032729591625	26.644802769426985	17.014749848857527
4	28.639717756962906	25.348207408879723	26.424838712348965	19.58723612180841
5	25.88486670476285	29.902523458139747	24.165569330915055	20.04704050618235
6	26.44786205868469	34.999792955518565	23.862621845677324	14.689723140119423
7	80.64697259559243	5.931244668604603	8.917157362087671	4.504625373715289
8	81.24077616835201	5.220005465974309	9.222258109932339	4.316960255741344
9	74.64102627808559	7.41351752010402	11.427199019437335	6.518257182373061
10	40.101700249281556	26.298293125295036	17.988852725119465	15.611153900303943
11	30.9198572221256	25.342410163399503	24.630177147258316	19.107555467216578
12	28.33064175507466	22.835184310997374	28.406668488658106	20.427505445269862
13	25.10985780185015	23.87537578573381	29.890432060423862	21.124334351992182
14	22.08054858505801	26.161975038717316	29.82732490248205	21.93015147374262
15	22.009822190199344	25.011635899856728	32.32544079770098	20.653101112242954
16	24.43969622433684	24.00589662683131	31.285911865305145	20.268495283526715
17	24.272073012165933	23.53681664968902	29.11956404713989	23.07154629100516
18	25.254457667685326	23.50153627005226	29.00660057806819	22.237405484194227
19	25.131224792334383	26.058287162413972	26.878017673316933	21.932470371934706
20	28.43731107191069	24.01119696555608	26.97607393972521	20.57541802280802
21	26.418875831283593	25.602954938839062	26.802487846488937	21.175681383388405
22	24.656513205297024	24.5586225744739	26.47121667619071	24.31364754403836
23	24.516551135846026	26.394030493511227	26.757766238498682	22.331652132144068
24	23.668000033127118	24.270085385144142	28.80088118131299	23.261033400415744
25	24.41683851358626	25.56419621191417	26.86725136028224	23.151713914217332
26	24.038361201520534	26.379620197603256	26.894746867417	22.687271733459216
27	24.543715371810478	25.31557719860535	27.057566647618575	23.083140781965596
28	23.094072730585438	26.779630135738365	27.770462206100362	22.35583492757584
29	24.650715959816807	25.072092888436153	27.38602201296927	22.891169138777776
30	25.809668149105153	23.963990823788585	27.754892461096343	22.471448566009922
31	25.501420325142654	25.21818347453767	26.611178745641713	22.669217454677963
32	25.97497246308397	25.241703727628845	27.373433708497934	21.40989010078925
33	24.868526754287892	25.14513818148691	27.52051810811035	22.46581695611485
34	24.009043702949143	25.612064896022265	28.77868601290301	21.600205388125584
35	24.58330227666112	25.00567301879136	28.12757252768185	22.28345217686568
36	23.718850157767896	27.32937464284827	26.82600809958011	22.125767099803724
37	24.852294466943277	26.427157610541048	27.371777352646443	21.34877056986923
38	23.754461808574952	25.587385193835043	28.231094768400045	22.42705822918996
39	24.163747339478412	24.0824202671702	27.836219533404556	23.91761285994683
40	24.875317813279004	23.77599443464434	29.381599542845787	21.96708820923087
41	22.331652132144068	25.197810297564327	28.611394071902406	23.859143498389194
42	23.815746975080128	25.48651312247923	27.22850257149246	23.469237330948182
43	23.93434205404689	24.757385276652837	28.008646177544783	23.299626491755486
44	24.294765087331363	24.896022261422644	27.147838041524842	23.661374609721154
45	24.036704845669043	23.66717185520137	27.68963204054759	24.606491258581993
46	23.497892287178978	24.932793361325746	27.085062154753324	24.48425219674195
47	23.62278151838141	24.955154165320877	28.7624537255584	22.659610590739316
48	23.30857081335354	23.853180617323826	29.028961382063322	23.80928718725931
49	23.21167399604131	24.687652695305058	28.212046676107892	23.888626632545737
50	21.719297373847798	24.57154215011553	29.525536866340364	24.183623609696305
51	21.96162223492095	24.105609249091074	28.333126288851894	25.599642227136076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	305.0
1	598.0
2	891.0
3	3471.5
4	6052.0
5	4109.0
6	2166.0
7	2050.5
8	1935.0
9	1937.5
10	1940.0
11	2007.0
12	2074.0
13	2036.0
14	1998.0
15	1896.0
16	1794.0
17	1817.0
18	1840.0
19	1777.0
20	1714.0
21	1774.5
22	1835.0
23	2021.5
24	2208.0
25	2494.5
26	3560.5
27	4340.0
28	4828.5
29	5317.0
30	6520.0
31	7723.0
32	8952.5
33	10182.0
34	11849.5
35	13517.0
36	15040.5
37	16564.0
38	17927.0
39	19290.0
40	21635.5
41	23981.0
42	25697.0
43	27413.0
44	30794.5
45	34176.0
46	37952.0
47	41728.0
48	44790.0
49	47852.0
50	49151.0
51	50450.0
52	47202.0
53	43954.0
54	41207.0
55	38460.0
56	36527.0
57	34594.0
58	33578.0
59	32562.0
60	31974.5
61	31387.0
62	29116.0
63	26845.0
64	23471.5
65	20098.0
66	16970.0
67	13842.0
68	11870.5
69	9899.0
70	8421.0
71	6943.0
72	6034.0
73	5125.0
74	4255.5
75	2866.0
76	2346.0
77	1722.5
78	1099.0
79	829.0
80	559.0
81	449.0
82	339.0
83	237.5
84	136.0
85	86.0
86	36.0
87	39.0
88	42.0
89	25.0
90	8.0
91	7.5
92	7.0
93	4.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.18139378576201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.25037282337607	24.35437050858173
2	7.694007006289401	5.259837665447798
3	3.3142111367761884	3.398530678659146
4	2.216202613729747	3.0301157699572596
5	1.8175981411304432	3.1064018903124357
6	1.5542431411799713	3.187571810849538
7	1.4308556488705646	3.423604826923875
8	1.2387105071097426	3.3872681304063255
9	1.189859259631992	3.6603943112804718
>10	8.220470133558146	43.71916879945433
>50	0.04962846365287026	1.1618471796816572
>100	0.021408356868897305	1.3399660358391823
>500	0.0019462142608088458	0.40895823285696287
>1k	4.8655356520221145E-4	0.5619641597493605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3379	0.5596826422188543	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTG	724	0.11992016364795813	TruSeq Adapter, Index 10 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGGTTTGTCTCGTATGCCGTCTTCTGC	649	0.10749749476177463	RNA PCR Primer, Index 10 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.3127117029822686E-4	0.0	0.0	0.0887806736399248	0.0
2	3.3127117029822686E-4	0.0	0.0	0.35545396572999743	0.0
3	3.3127117029822686E-4	0.0	0.0	0.636703189313192	0.0
4	3.3127117029822686E-4	0.0	0.0	0.8699180932031437	0.0
5	3.3127117029822686E-4	0.0	0.0	1.5195408581579666	0.0
6	3.3127117029822686E-4	0.0	0.0	2.27185768590524	0.0
7	3.3127117029822686E-4	0.0	0.0	2.8570482082370576	0.0
8	3.3127117029822686E-4	0.0	0.0	3.796533247202829	0.0
9	3.3127117029822686E-4	0.0	0.0	4.301224875152178	0.0
10	3.3127117029822686E-4	0.0	0.0	5.065136193859889	0.0
11	3.3127117029822686E-4	0.0	0.0	5.918325092962972	0.0
12	3.3127117029822686E-4	0.0	0.0	6.586167772284197	0.0
13	3.3127117029822686E-4	0.0	0.0	6.923236188062643	0.0
14	3.3127117029822686E-4	0.0	0.0	7.094503383106827	0.0
15	3.3127117029822686E-4	0.0	0.0	7.338981506786918	0.0
16	3.3127117029822686E-4	0.0	0.0	7.752407927319105	0.0
17	3.3127117029822686E-4	0.0	0.0	8.212874854033641	0.0
18	3.3127117029822686E-4	0.0	0.0	8.705309448681955	0.0
19	3.3127117029822686E-4	0.0	0.0	9.000472061417675	0.0
20	3.3127117029822686E-4	0.0	0.0	9.301100648463317	0.0
21	3.3127117029822686E-4	0.0	0.0	9.674111986219119	0.0
22	3.3127117029822686E-4	0.0	0.0	10.077765907227509	0.0
23	3.3127117029822686E-4	0.0	0.0	10.479763472384407	0.0
24	3.3127117029822686E-4	0.0	0.0	10.8267700232718	0.0
25	3.3127117029822686E-4	0.0	0.0	11.143962168832353	0.0
26	3.3127117029822686E-4	0.0	0.0	11.444259484707695	0.0
27	3.3127117029822686E-4	0.0	0.0	11.756151291543475	0.0
28	3.3127117029822686E-4	0.0	0.0	12.073509072689177	0.0
29	3.3127117029822686E-4	0.0	0.0	12.406105327668596	0.0
30	3.3127117029822686E-4	0.0	0.0	12.822678824318617	0.0
31	3.3127117029822686E-4	0.0	0.0	13.17697334095257	0.0
32	3.3127117029822686E-4	0.0	0.0	13.559922813817321	0.0
33	3.3127117029822686E-4	0.0	0.0	13.884899831879881	0.0
34	3.3127117029822686E-4	0.0	0.0	14.206729773824609	0.0
35	3.3127117029822686E-4	0.0	0.0	14.547110901306036	0.0
36	3.3127117029822686E-4	0.0	0.0	14.885173130595376	0.0
37	3.3127117029822686E-4	0.0	0.0	15.233173494993665	0.0
38	3.3127117029822686E-4	0.0	0.0	15.604528476897977	0.0
39	3.3127117029822686E-4	0.0	0.0	15.977705450238929	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCGT	20	7.0306234E-4	45.0	45
CCCAATC	30	2.1637825E-6	44.999996	44
CGTTTTT	1885	0.0	40.225464	1
ATAAGCG	35	6.243361E-6	38.571426	1
CGTAAGG	65	9.094947E-12	38.076927	2
TTAAGCA	95	0.0	37.894737	45
CATACGA	60	1.546141E-10	37.499996	18
GCGATAT	30	1.13928014E-4	37.499996	9
TCGATCA	85	0.0	37.058823	17
ACACGAC	365	0.0	36.986298	26
TTAATCG	55	2.744855E-9	36.81818	20
CGTTGAT	55	2.744855E-9	36.81818	25
GGCCGAT	110	0.0	36.81818	8
TAATCGT	55	2.744855E-9	36.81818	21
CGACCAA	375	0.0	36.0	29
AAACACG	350	0.0	36.0	40
CGAGACA	375	0.0	36.0	22
ACGTAAG	25	0.0021063178	36.0	1
GGACCGT	25	0.0021063178	36.0	8
CCAACGG	25	0.0021063178	36.0	2
>>END_MODULE