SRR3550343_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451951
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1702	0.3765894975340247	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1437	0.3179548225360714	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	979	0.21661640310564642	RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.13674048735371755	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	560	0.123907237731524	Illumina PCR Primer Index 7 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	518	0.1146141949016597	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2097371E-7	45.0	1
TCACGAC	25	3.887037E-5	45.0	25
GAATGCG	20	7.028739E-4	45.0	1
ATATGCG	35	1.2097371E-7	45.0	1
TACGCGG	20	7.028739E-4	45.0	2
TCCACGG	20	7.028739E-4	45.0	2
CTCGCGA	20	7.028739E-4	45.0	27
GTATCGG	20	7.028739E-4	45.0	2
GCGATAT	25	3.887037E-5	45.0	9
TCACTAG	20	7.028739E-4	45.0	1
CGGCTAA	20	7.028739E-4	45.0	29
ACGTAGG	55	1.8189894E-12	45.0	2
GCGGAAT	25	3.887037E-5	45.0	5
ACCCGAA	20	7.028739E-4	45.0	15
ACGAGCA	20	7.028739E-4	45.0	43
GGCCTTA	20	7.028739E-4	45.0	24
CGATAGT	20	7.028739E-4	45.0	10
TACGTTG	20	7.028739E-4	45.0	1
TAATCCG	20	7.028739E-4	45.0	1
AACACGT	115	0.0	43.04348	41