##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550343_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	451951
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.181048387988966	33.0	31.0	34.0	30.0	34.0
2	32.28763295135977	34.0	31.0	34.0	30.0	34.0
3	32.39639695453711	34.0	31.0	34.0	30.0	34.0
4	35.90395197709486	37.0	35.0	37.0	35.0	37.0
5	35.87061429225734	37.0	35.0	37.0	35.0	37.0
6	35.89293529608298	37.0	35.0	37.0	35.0	37.0
7	36.07603479138225	37.0	35.0	37.0	35.0	37.0
8	36.04205765669287	37.0	35.0	37.0	35.0	37.0
9	37.61639425512943	39.0	38.0	39.0	35.0	39.0
10	37.375976599233105	39.0	37.0	39.0	34.0	39.0
11	37.32262347024346	39.0	37.0	39.0	34.0	39.0
12	37.20508639210888	39.0	37.0	39.0	34.0	39.0
13	37.0959727935108	39.0	37.0	39.0	33.0	39.0
14	38.24227183920381	40.0	37.0	41.0	33.0	41.0
15	38.32624111905937	40.0	37.0	41.0	34.0	41.0
16	38.39170839316652	40.0	38.0	41.0	34.0	41.0
17	38.42221833782866	40.0	37.0	41.0	34.0	41.0
18	38.39803430017856	40.0	37.0	41.0	34.0	41.0
19	38.35536595781401	40.0	37.0	41.0	34.0	41.0
20	38.24193994481703	40.0	37.0	41.0	34.0	41.0
21	38.18589847129446	40.0	37.0	41.0	34.0	41.0
22	38.16262603689338	40.0	37.0	41.0	34.0	41.0
23	38.12893875663512	40.0	37.0	41.0	34.0	41.0
24	38.10907377127167	40.0	37.0	41.0	34.0	41.0
25	38.05921217123095	40.0	36.0	41.0	34.0	41.0
26	37.92727308933933	40.0	36.0	41.0	34.0	41.0
27	37.87733847253352	40.0	36.0	41.0	33.0	41.0
28	37.798834386913626	40.0	36.0	41.0	33.0	41.0
29	37.7429323090335	40.0	36.0	41.0	33.0	41.0
30	37.72154503474934	40.0	36.0	41.0	33.0	41.0
31	37.6068533978241	40.0	36.0	41.0	33.0	41.0
32	37.446459903839134	40.0	36.0	41.0	33.0	41.0
33	37.36790935300508	40.0	35.0	41.0	33.0	41.0
34	37.20531208029189	39.0	35.0	41.0	32.0	41.0
35	37.22381187341106	39.0	35.0	41.0	32.0	41.0
36	37.064126420784554	39.0	35.0	41.0	32.0	41.0
37	37.01433783750893	39.0	35.0	41.0	31.0	41.0
38	36.93629619140128	39.0	35.0	41.0	31.0	41.0
39	36.84141422410836	39.0	35.0	41.0	31.0	41.0
40	36.721295007644635	39.0	35.0	41.0	31.0	41.0
41	36.62523813422251	39.0	35.0	41.0	31.0	41.0
42	36.56850853300468	39.0	35.0	41.0	31.0	41.0
43	36.50413651037391	39.0	35.0	41.0	31.0	41.0
44	36.298373053716	39.0	35.0	41.0	30.0	41.0
45	36.248823434398865	39.0	35.0	40.0	30.0	41.0
46	36.12623713632673	38.0	35.0	40.0	30.0	41.0
47	36.033667366595054	38.0	35.0	40.0	30.0	41.0
48	35.92823337043175	38.0	35.0	40.0	30.0	41.0
49	35.843255131640376	38.0	35.0	40.0	29.0	41.0
50	35.71957579472111	38.0	35.0	40.0	29.0	41.0
51	34.400948332894494	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	2.0
12	1.0
13	3.0
14	4.0
15	11.0
16	28.0
17	49.0
18	74.0
19	166.0
20	271.0
21	457.0
22	705.0
23	1124.0
24	1657.0
25	2368.0
26	3307.0
27	4033.0
28	4754.0
29	5776.0
30	7098.0
31	8902.0
32	11572.0
33	15789.0
34	27408.0
35	38468.0
36	33973.0
37	49705.0
38	86742.0
39	147394.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30458169137805	20.395131330608848	23.65101526492916	11.649271713083941
2	31.993070045204014	24.944739584600985	24.371226084243645	18.690964285951353
3	33.061106181864844	24.627669813762996	25.433730647791464	16.877493356580693
4	29.555416405760802	25.56516082495669	24.701129104703828	20.178293664578682
5	25.46050346165845	30.77103491307686	22.828359711561653	20.940101913703035
6	26.82967843859179	35.212003071129395	22.917749933067967	15.040568557210849
7	80.21754570738864	6.330111007609232	8.131855001980304	5.320488283021832
8	79.21301203006522	5.911260291491777	8.885033997048353	5.9906936813946645
9	72.86652756604146	8.020559750946452	11.325121528661294	7.787791154350804
10	39.1936293978772	26.76219324661302	18.31127710747404	15.732900248035738
11	30.677440696004656	24.798927317341924	24.553325471124083	19.970306515529337
12	28.348205889576526	22.509962363176538	28.891848895123584	20.24998285212335
13	24.943854532902904	24.74405411206082	29.58572942642012	20.726361928616154
14	19.697931855444505	28.37409365174543	29.225955911149658	22.7020185816604
15	18.646711701047238	25.97073576560291	34.33602315295242	21.046529380397434
16	20.69007480899478	24.615279089989844	32.08024763746513	22.614398463550252
17	20.378315348345286	24.37365997641337	28.688508267489176	26.55951640775217
18	22.699142163641632	24.521242347068597	29.96541660489743	22.814198884392333
19	23.556314733234355	26.323650130213235	26.75511283302836	23.364922303524054
20	26.550002101997784	25.665392929764508	27.16444924339143	20.620155724846278
21	24.06588324840525	26.546461895205454	27.691276266674926	21.69637858971437
22	22.55709136609942	25.89838278928468	25.73376317344137	25.81076267117453
23	21.234381603315402	26.925485284909207	27.20803803952198	24.632095072253406
24	22.578553869777917	23.832672125960556	29.467132498877092	24.121641505384435
25	21.069983250396614	26.425431075492696	26.928361702927972	25.576223971182717
26	20.84628643370631	28.2844821673146	27.428194649419957	23.44103674955913
27	23.427097185314338	26.52566318030052	27.361594509139266	22.68564512524588
28	20.072751249582367	26.500439206905174	28.89406152436879	24.532748019143668
29	21.532201499720102	26.421005817002285	27.57046670988669	24.47632597339092
30	23.579768603233536	25.81872813645727	25.88046049239851	24.72104276791068
31	22.96421514721729	28.046181997605935	24.59381658631135	24.39578626886543
32	22.62147887713491	27.200293837163763	24.986779540259896	25.19144774544143
33	22.431192762047214	26.364141245400496	24.520357295370516	26.684308697181773
34	20.33428402636569	27.302517308292266	27.14542063188266	25.217778033459382
35	21.39656732698899	25.615387508822856	24.804016364605896	28.184028799582254
36	21.74549895895794	27.383057012817762	24.891415219791526	25.98002880843277
37	21.720717511411635	27.29012658451912	27.08368827594142	23.905467628127827
38	20.53364192135873	26.141550743332797	25.347659370152957	27.977147965155513
39	21.899276691499743	24.654000101780944	25.545468424674354	27.901254782044955
40	22.604441631946827	25.164896194498958	27.645474841299166	24.585187332255046
41	20.20041995703074	26.081145964938678	25.820498239853436	27.897935838177148
42	22.016546041495648	25.17485302610239	26.2437742144613	26.564826717940658
43	23.12551581919279	23.925160028410158	27.110239826883888	25.839084325513163
44	21.204511108505127	24.501992472635308	27.10360193914827	27.189894479711295
45	21.56671851594531	24.248646424059245	25.78000712466617	28.404627935329273
46	21.96100904744098	25.328630758644188	26.799807943781516	25.91055225013331
47	20.26945398948116	24.819947295171378	28.93654400587674	25.974054709470717
48	20.833010658235075	23.459401572294343	27.81075824591604	27.896829523554544
49	21.375547349159532	22.93191076023728	28.723246546638904	26.969295343964276
50	19.332848029985552	23.539056225121747	28.75621472239247	28.37188102250023
51	20.1785149275032	23.19167343362444	25.651674628444233	30.97813701042812
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	414.0
2	684.0
3	1057.0
4	1430.0
5	1049.0
6	668.0
7	690.0
8	712.0
9	767.0
10	822.0
11	858.5
12	895.0
13	947.0
14	999.0
15	912.0
16	825.0
17	872.0
18	919.0
19	948.5
20	978.0
21	1015.5
22	1053.0
23	1342.0
24	1631.0
25	1784.0
26	2179.5
27	2422.0
28	3295.0
29	4168.0
30	4908.5
31	5649.0
32	6805.0
33	7961.0
34	8707.0
35	9453.0
36	10584.5
37	11716.0
38	12663.5
39	13611.0
40	15565.0
41	17519.0
42	20041.0
43	22563.0
44	24292.0
45	26021.0
46	29628.5
47	33236.0
48	36394.5
49	39553.0
50	44064.5
51	48576.0
52	48079.0
53	47582.0
54	41333.5
55	35085.0
56	30136.5
57	25188.0
58	23184.0
59	21180.0
60	19703.0
61	18226.0
62	17171.5
63	16117.0
64	13443.5
65	10770.0
66	9209.5
67	7649.0
68	6265.5
69	4882.0
70	4211.0
71	3540.0
72	2797.5
73	2055.0
74	1765.0
75	1256.0
76	1037.0
77	809.0
78	581.0
79	413.0
80	245.0
81	187.5
82	130.0
83	76.0
84	22.0
85	24.0
86	26.0
87	16.5
88	7.0
89	5.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	451951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.95308903218488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0330590218404	23.73711807207335
2	11.02960709059295	7.269192488926543
3	4.089356692089321	4.0427080547634135
4	1.9157125275226352	2.525145819181013
5	1.1300725892625842	1.8619691323400813
6	0.8323689678533607	1.6457477223179775
7	0.6320283682570621	1.457910096302905
8	0.5287765239419188	1.393985589726983
9	0.48403983066816325	1.4355546871618508
>10	7.182990132752592	48.871566327120405
>50	0.09421021673298272	2.091114395098599
>100	0.043740457768884834	2.378725781147685
>500	0.0026917204780852206	0.5931846242724022
>1k	0.0013458602390426103	0.6960772095667552
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1702	0.3765894975340247	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	1437	0.3179548225360714	RNA PCR Primer, Index 16 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	979	0.21661640310564642	RNA PCR Primer, Index 16 (96% over 26bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	618	0.13674048735371755	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	560	0.123907237731524	Illumina PCR Primer Index 7 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	518	0.1146141949016597	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.637887735617357E-4	0.0	0.0	0.24272542819907467	0.0
2	6.637887735617357E-4	0.0	0.0	1.2532332044845569	0.0
3	6.637887735617357E-4	0.0	0.0	1.740454164278871	0.0
4	6.637887735617357E-4	0.0	0.0	2.2628559290719568	0.0
5	6.637887735617357E-4	0.0	0.0	3.9203364966556107	0.0
6	6.637887735617357E-4	0.0	0.0	4.848313202094918	0.0
7	6.637887735617357E-4	0.0	0.0	5.818551126117654	0.0
8	6.637887735617357E-4	0.0	0.0	7.435319315589522	0.0
9	6.637887735617357E-4	0.0	0.0	8.109286183679204	0.0
10	6.637887735617357E-4	0.0	0.0	9.49240072485734	0.0
11	6.637887735617357E-4	0.0	0.0	11.355213286396092	0.0
12	6.637887735617357E-4	0.0	0.0	12.736557724178063	0.0
13	6.637887735617357E-4	0.0	0.0	13.28595356576266	0.0
14	6.637887735617357E-4	0.0	0.0	13.514296903867898	0.0
15	6.637887735617357E-4	0.0	0.0	13.984259355549606	0.0
16	6.637887735617357E-4	0.0	0.0	14.954718542496863	0.0
17	8.850516980823143E-4	0.0	0.0	16.080283039533047	0.0
18	8.850516980823143E-4	0.0	0.0	17.24456854836033	0.0
19	8.850516980823143E-4	0.0	0.0	17.885567240696446	0.0
20	8.850516980823143E-4	0.0	0.0	18.50156322256174	0.0
21	8.850516980823143E-4	0.0	0.0	19.29523333281705	0.0
22	8.850516980823143E-4	0.0	0.0	20.163247785711285	0.0
23	8.850516980823143E-4	0.0	0.0	21.004931950587565	0.0
24	8.850516980823143E-4	0.0	0.0	21.694165960469167	0.0
25	8.850516980823143E-4	0.0	0.0	22.259050206770205	0.0
26	8.850516980823143E-4	0.0	0.0	22.786098492978223	0.0
27	8.850516980823143E-4	0.0	0.0	23.307615206073226	0.0
28	8.850516980823143E-4	0.0	0.0	23.836212332752886	0.0
29	8.850516980823143E-4	0.0	0.0	24.425214237826665	0.0
30	8.850516980823143E-4	0.0	0.0	25.120422346670324	0.0
31	8.850516980823143E-4	0.0	0.0	25.71960234627205	0.0
32	8.850516980823143E-4	0.0	0.0	26.302851415308297	0.0
33	8.850516980823143E-4	0.0	0.0	26.857557567081386	0.0
34	8.850516980823143E-4	0.0	0.0	27.424433179703108	0.0
35	8.850516980823143E-4	0.0	0.0	28.004584567796066	0.0
36	8.850516980823143E-4	0.0	0.0	28.56393724098409	0.0
37	8.850516980823143E-4	0.0	0.0	29.119528444455263	0.0
38	8.850516980823143E-4	0.0	0.0	29.63861126538054	0.0
39	8.850516980823143E-4	0.0	0.0	30.180926693380478	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	35	1.2097371E-7	45.0	1
TCACGAC	25	3.887037E-5	45.0	25
GAATGCG	20	7.028739E-4	45.0	1
ATATGCG	35	1.2097371E-7	45.0	1
TACGCGG	20	7.028739E-4	45.0	2
TCCACGG	20	7.028739E-4	45.0	2
CTCGCGA	20	7.028739E-4	45.0	27
GTATCGG	20	7.028739E-4	45.0	2
GCGATAT	25	3.887037E-5	45.0	9
TCACTAG	20	7.028739E-4	45.0	1
CGGCTAA	20	7.028739E-4	45.0	29
ACGTAGG	55	1.8189894E-12	45.0	2
GCGGAAT	25	3.887037E-5	45.0	5
ACCCGAA	20	7.028739E-4	45.0	15
ACGAGCA	20	7.028739E-4	45.0	43
GGCCTTA	20	7.028739E-4	45.0	24
CGATAGT	20	7.028739E-4	45.0	10
TACGTTG	20	7.028739E-4	45.0	1
TAATCCG	20	7.028739E-4	45.0	1
AACACGT	115	0.0	43.04348	41
>>END_MODULE