FastQCFastQC Report
Sat 18 Jun 2016
SRR3550342_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550342_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674201
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21730.32230744243927256No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC21410.3175610834157766No Hit
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC18140.26905922714442726No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT14330.21254789002092847No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG8610.1277067224759382No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTACG207.0312084E-445.0000041
TATGCAC253.889083E-545.00000418
ATCGTCC207.0312084E-445.00000433
ACGATAG253.889083E-545.0000041
ACGTAAC207.0312084E-445.00000435
GTCCAAC406.8084773E-945.00000418
TATACGG207.0312084E-445.0000042
CGATATT207.0312084E-445.00000410
CCTTATC600.044.99999643
AACATCG302.1641627E-644.9999961
AATTGCG302.1641627E-644.9999961
TAGTACG302.1641627E-644.9999961
GCTACGA1050.040.71428710
AATGCGA451.9263098E-840.025
CGCGGGT403.4553887E-739.3750044
ATTAGCG356.244447E-638.5714261
CGTTTTT11850.038.5443041
TCAAGCG5800.038.0172417
CACGTGA5300.037.7830243
GTGGCGA1550.037.74193628