FastQCFastQC Report
Sat 18 Jun 2016
SRR3550341_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550341_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791823
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78810.9952981916413137No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG27080.3419956227591267No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT25560.32279941350529096No Hit
CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC21580.27253565506432625No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT18810.23755308951621765No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC18340.23161741954957105No Hit
CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT18250.23048080189638342No Hit
GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC16000.2020653605666923No Hit
CGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT12390.15647436358883235No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC9460.11947114443505683No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC8630.10898900385565966No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGTG351.2112832E-745.0000041
GCGAAGT207.031956E-445.043
TCGATTG253.889703E-545.01
TAACGCC453.8380676E-1045.012
TATAGCG253.889703E-545.01
ATAACGC453.8380676E-1045.011
CGCGTAA207.031956E-445.031
CGACTAT253.889703E-545.030
TAGTACG207.031956E-445.01
CGTTTTT46800.043.5096171
TACGGGT650.041.5384644
CGTTCTG2800.040.9821431
TTAGGCG556.002665E-1140.9090921
CGTTTCT3500.040.51
ATCGCCA501.0804797E-940.524
AACGGGC900.040.04
TACGCGG451.9270374E-840.02
ATTCTCG356.2458257E-638.5714313
TACGAAT659.094947E-1238.07692712
CACGCGT301.1396011E-437.50000439