##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550341_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 791823 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08453530650158 33.0 31.0 34.0 30.0 34.0 2 32.21190594362629 34.0 31.0 34.0 30.0 34.0 3 32.25415402179527 34.0 31.0 34.0 30.0 34.0 4 35.827909772764876 37.0 35.0 37.0 35.0 37.0 5 35.81743899836201 37.0 35.0 37.0 35.0 37.0 6 35.83880235860792 37.0 35.0 37.0 35.0 37.0 7 36.049207966932 37.0 35.0 37.0 35.0 37.0 8 36.05644190684029 37.0 35.0 37.0 35.0 37.0 9 37.66959787730339 39.0 38.0 39.0 35.0 39.0 10 37.357592542777866 39.0 37.0 39.0 34.0 39.0 11 37.31766442752989 39.0 37.0 39.0 34.0 39.0 12 37.22808506446516 39.0 37.0 39.0 34.0 39.0 13 37.186770275680296 39.0 37.0 39.0 34.0 39.0 14 38.36173740848649 40.0 38.0 41.0 34.0 41.0 15 38.401537969975614 40.0 38.0 41.0 34.0 41.0 16 38.41013079943371 40.0 38.0 41.0 34.0 41.0 17 38.41402560925864 40.0 38.0 41.0 34.0 41.0 18 38.30696759250489 40.0 37.0 41.0 34.0 41.0 19 38.18269486993937 40.0 37.0 41.0 34.0 41.0 20 38.00344142567215 40.0 36.0 41.0 34.0 41.0 21 37.93631026125788 40.0 36.0 41.0 34.0 41.0 22 37.92268221559616 40.0 36.0 41.0 34.0 41.0 23 37.88508921817123 40.0 36.0 41.0 34.0 41.0 24 37.85585541213125 40.0 36.0 41.0 33.0 41.0 25 37.819979970271135 40.0 36.0 41.0 33.0 41.0 26 37.67349773876232 40.0 35.0 41.0 33.0 41.0 27 37.596936436552106 40.0 35.0 41.0 33.0 41.0 28 37.49681557620832 39.0 35.0 41.0 33.0 41.0 29 37.41469368785701 39.0 35.0 41.0 33.0 41.0 30 37.30561249168059 39.0 35.0 41.0 33.0 41.0 31 37.1349973415776 39.0 35.0 41.0 32.0 41.0 32 36.86586396202181 39.0 35.0 41.0 31.0 41.0 33 36.58444879726909 39.0 35.0 41.0 30.0 41.0 34 36.281463155275866 39.0 35.0 41.0 30.0 41.0 35 36.12699302748215 39.0 35.0 41.0 29.0 41.0 36 35.93013463867556 39.0 35.0 41.0 29.0 41.0 37 35.87754965440509 39.0 35.0 41.0 28.0 41.0 38 35.78458569655087 39.0 35.0 41.0 27.0 41.0 39 35.70241708058493 39.0 35.0 41.0 27.0 41.0 40 35.606395621243635 39.0 35.0 41.0 27.0 41.0 41 35.445765025769646 38.0 35.0 40.0 26.0 41.0 42 35.3915496266211 38.0 35.0 40.0 26.0 41.0 43 35.30526266602511 38.0 35.0 40.0 25.0 41.0 44 35.12878256883167 38.0 35.0 40.0 24.0 41.0 45 35.03377396211022 38.0 34.0 40.0 24.0 41.0 46 34.891526262813784 38.0 34.0 40.0 23.0 41.0 47 34.75800273545982 38.0 34.0 40.0 23.0 41.0 48 34.62819721074028 37.0 34.0 40.0 23.0 41.0 49 34.53599857543921 37.0 34.0 40.0 23.0 41.0 50 34.44359913768607 37.0 34.0 40.0 23.0 41.0 51 33.06773104595345 36.0 31.0 39.0 19.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 6.0 12 2.0 13 10.0 14 18.0 15 13.0 16 34.0 17 85.0 18 194.0 19 383.0 20 737.0 21 1241.0 22 1975.0 23 3106.0 24 5084.0 25 8042.0 26 11499.0 27 12927.0 28 13250.0 29 13817.0 30 15324.0 31 17896.0 32 21929.0 33 28560.0 34 47442.0 35 62516.0 36 61740.0 37 87752.0 38 147859.0 39 228242.0 40 138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.957006805813926 19.976307836473556 23.26353238034258 15.803152977369942 2 36.4619365691575 22.622227442244036 23.411797838658387 17.504038149940072 3 29.70878592816829 23.15302788628267 30.56175433146044 16.576431854088604 4 26.71619793817558 24.533134298953176 29.835329360223184 18.915338402648064 5 23.675493134197918 28.643775187131464 28.350148960057993 19.330582718612618 6 24.757553140032556 32.350032772475664 27.96332008542313 14.929094002068643 7 76.87387206484277 5.005285272087322 12.987751050424148 5.133091612645756 8 77.03767129775215 4.219629892033952 13.223283486334699 5.5194153238792 9 70.64217634496597 6.171328693407491 15.706414185998637 7.480080775627887 10 37.16613435073242 23.752656843764324 22.45767046423254 16.623538341270713 11 27.72109423444381 23.35446179259759 28.382731999449373 20.54171197350923 12 24.431091291866995 21.477779756334435 32.70491006197092 21.386218889827653 13 22.938333440680555 23.085714863043886 34.70813553028896 19.26781616598659 14 20.319818949436932 25.363370349181576 33.23785745046557 21.078953250915923 15 19.65578165827464 24.235971928069784 35.62172354175112 20.48652287190445 16 22.346534515920858 24.364030850328923 33.174080571036704 20.11535406271351 17 21.20271828426302 24.60714073726073 30.776448777062548 23.4136922014137 18 23.059320075319864 23.64265751310583 31.825294289254035 21.472728122320266 19 21.803989022799286 27.3509357520557 29.229891023625232 21.615184201519785 20 23.613358035823662 25.898843554683303 29.484493377939263 21.00330503155377 21 22.05770734116084 27.2332326795256 30.352237810722848 20.356822168590707 22 21.549891831886672 23.661095977257542 29.667615110952827 25.12139707990296 23 20.976657662129035 26.114422036237894 29.678728705783996 23.230191595849075 24 21.311833578969036 23.835124769045606 31.590267016744907 23.26277463524045 25 21.886583238930925 25.498122686509483 28.834221789465573 23.78107228509402 26 21.29314253311662 25.251729237468474 30.223421143361584 23.231707086053323 27 22.05657072350765 24.187476241533776 30.137038201719324 23.618914833239245 28 20.56974854228786 24.835095722150026 30.63816029592472 23.956995439637392 29 22.92279966608699 22.74346665858405 29.79744210511693 24.53629157021203 30 24.33612057240065 22.467394859709806 30.769629071143424 22.426855496746118 31 23.368858949537966 24.200863071671318 28.420492963705275 24.00978501508544 32 23.05767829426526 24.07166753175899 29.837855177230267 23.032798996745484 33 22.14068042984354 23.808224817920166 30.247416404928877 23.803678347307418 34 21.186047892016273 23.905216190992178 31.4331611989043 23.47557471808725 35 21.538146782803732 24.193159329799716 31.996670973184667 22.272022914211888 36 22.027650118776545 26.005433032382236 29.92411182802217 22.04280502081905 37 20.667497660461997 25.256528289781933 32.231950827394506 21.844023222361564 38 20.149957755710556 26.44454631906373 30.942268663577593 22.46322726164812 39 22.691687409938837 22.957655940784747 31.257111753510568 23.093544895765845 40 23.02661074507813 23.359387135761402 32.003616969954145 21.610385149206326 41 20.213734635139417 25.112177847827105 30.546978301969002 24.127109215064475 42 21.263843055834446 24.64894300872796 31.01046572276885 23.07674821266874 43 21.500385818547834 22.882512884824006 31.797762883876825 23.819338412751335 44 22.616291772277393 23.037219176507882 29.227870370019566 25.118618681195166 45 22.927346136699743 21.227218709231735 30.15598182927245 25.689453324796073 46 23.32617264211825 22.74485585793795 29.364769651803496 24.564201848140303 47 20.57631566650628 23.379214799267007 32.40610591003292 23.63836362419379 48 20.601573836577113 22.303090463399016 32.39890733156274 24.696428368461135 49 22.178315103249087 21.289732680157055 32.019782198799476 24.51217001779438 50 20.25212705364709 20.953167563963156 32.912405929102846 25.88229945328691 51 20.575557921404155 20.81816264493454 30.604693220580863 28.001586213080447 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 303.0 1 613.5 2 924.0 3 6454.5 4 11985.0 5 8013.0 6 4041.0 7 3916.5 8 3792.0 9 3897.5 10 4003.0 11 4019.5 12 4036.0 13 4036.5 14 4037.0 15 3982.5 16 3928.0 17 3721.5 18 3515.0 19 3322.0 20 3129.0 21 3105.0 22 3081.0 23 3133.5 24 3186.0 25 3441.0 26 4024.0 27 4352.0 28 5274.0 29 6196.0 30 7048.5 31 7901.0 32 9101.5 33 10302.0 34 12197.5 35 14093.0 36 15667.5 37 17242.0 38 19315.5 39 21389.0 40 25723.5 41 30058.0 42 36195.0 43 42332.0 44 55159.5 45 67987.0 46 78223.5 47 88460.0 48 88550.5 49 88641.0 50 78168.5 51 67696.0 52 60613.5 53 53531.0 54 48760.5 55 43990.0 56 40992.5 57 37995.0 58 34778.5 59 31562.0 60 29577.0 61 27592.0 62 24962.5 63 22333.0 64 19864.5 65 17396.0 66 14657.0 67 11918.0 68 9990.0 69 8062.0 70 6925.5 71 5789.0 72 5196.0 73 4603.0 74 3646.5 75 2383.5 76 2077.0 77 1623.0 78 1169.0 79 818.5 80 468.0 81 328.5 82 189.0 83 135.5 84 82.0 85 60.0 86 38.0 87 28.0 88 18.0 89 12.0 90 6.0 91 4.5 92 3.0 93 4.5 94 6.0 95 3.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 791823.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.26013740076438 #Duplication Level Percentage of deduplicated Percentage of total 1 76.05256774366704 31.379393947846435 2 9.427081288543087 7.7792533850692545 3 3.4799750728752805 4.307527489742073 4 1.9194578466597125 3.167883779526203 5 1.238870788351952 2.555798947459741 6 0.9702505260898698 2.4019602011779178 7 0.7586626777551427 2.1911768427506315 8 0.6751411938850815 2.2285134739691657 9 0.5756105340686021 2.1374792752498104 >10 4.7984500539424735 34.236055851056264 >50 0.07010818170239738 2.007235693157708 >100 0.02982669971422628 2.2179678845583704 >500 0.0012299669985210526 0.3851819298425475 >1k 0.002459933997042105 2.0046968621350767 >5k 3.0749174963026315E-4 0.9998744364588659 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7881 0.9952981916413137 No Hit CGCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTG 2708 0.3419956227591267 No Hit CGTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTT 2556 0.32279941350529096 No Hit CCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC 2158 0.27253565506432625 No Hit CGTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCT 1881 0.23755308951621765 No Hit CGTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTC 1834 0.23161741954957105 No Hit CTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGCT 1825 0.23048080189638342 No Hit GCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCTGC 1600 0.2020653605666923 No Hit CGTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTCTTCT 1239 0.15647436358883235 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCC 946 0.11947114443505683 No Hit CGTTTTTCTGTCTCTTATACACATCTGACGCGTCTGTATTCGTATGCCGTC 863 0.10898900385565966 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.262908503541827E-4 0.0 0.0 0.2638215863898876 0.0 2 1.262908503541827E-4 0.0 0.0 0.963599188202414 0.0 3 1.262908503541827E-4 0.0 0.0 1.6905293228410896 0.0 4 1.262908503541827E-4 0.0 0.0 2.3641647186303 0.0 5 1.262908503541827E-4 0.0 0.0 4.157494793659694 0.0 6 1.262908503541827E-4 0.0 0.0 5.884522172253142 0.0 7 1.262908503541827E-4 0.0 0.0 7.351137817416266 0.0 8 1.262908503541827E-4 0.0 0.0 9.518793972895457 0.0 9 1.262908503541827E-4 0.0 0.0 10.565618831481277 0.0 10 1.262908503541827E-4 0.0 0.0 12.284058432250642 0.0 11 1.262908503541827E-4 0.0 0.0 14.27831725019354 0.0 12 1.262908503541827E-4 0.0 0.0 15.800500869512504 0.0 13 1.262908503541827E-4 0.0 0.0 16.53197747476393 0.0 14 1.262908503541827E-4 0.0 0.0 16.86197546673941 0.0 15 1.262908503541827E-4 0.0 0.0 17.332914047710158 0.0 16 1.262908503541827E-4 0.0 0.0 18.224780032911397 0.0 17 1.262908503541827E-4 0.0 0.0 19.32161606823747 0.0 18 1.262908503541827E-4 0.0 0.0 20.457854848874053 0.0 19 1.262908503541827E-4 0.0 0.0 21.183395784158833 0.0 20 1.262908503541827E-4 0.0 0.0 21.85778892505017 0.0 21 1.262908503541827E-4 0.0 0.0 22.709494419838776 0.0 22 1.262908503541827E-4 0.0 0.0 23.584816303643617 0.0 23 1.262908503541827E-4 0.0 0.0 24.507749838031984 0.0 24 1.262908503541827E-4 0.0 0.0 25.242131132841557 0.0 25 1.262908503541827E-4 0.0 0.0 25.91526136522935 0.0 26 1.262908503541827E-4 0.0 0.0 26.530676679005282 0.0 27 1.262908503541827E-4 0.0 0.0 27.141166649617404 0.0 28 1.262908503541827E-4 0.0 0.0 27.765674904618834 0.0 29 1.262908503541827E-4 0.0 0.0 28.41948263690244 0.0 30 1.262908503541827E-4 0.0 0.0 29.20008638294164 0.0 31 1.262908503541827E-4 0.0 0.0 29.829646271957245 0.0 32 1.262908503541827E-4 0.0 0.0 30.53207598162721 0.0 33 1.262908503541827E-4 0.0 0.0 31.177927390338496 0.0 34 1.262908503541827E-4 0.0 0.0 31.77376762230953 0.0 35 1.262908503541827E-4 0.0 0.0 32.42378662908251 0.0 36 1.262908503541827E-4 0.0 0.0 33.02177380550957 0.0 37 1.262908503541827E-4 0.0 0.0 33.642872207551434 0.0 38 1.262908503541827E-4 0.0 0.0 34.27015886126066 0.0 39 1.262908503541827E-4 0.0 0.0 34.8963088973167 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTG 35 1.2112832E-7 45.000004 1 GCGAAGT 20 7.031956E-4 45.0 43 TCGATTG 25 3.889703E-5 45.0 1 TAACGCC 45 3.8380676E-10 45.0 12 TATAGCG 25 3.889703E-5 45.0 1 ATAACGC 45 3.8380676E-10 45.0 11 CGCGTAA 20 7.031956E-4 45.0 31 CGACTAT 25 3.889703E-5 45.0 30 TAGTACG 20 7.031956E-4 45.0 1 CGTTTTT 4680 0.0 43.509617 1 TACGGGT 65 0.0 41.538464 4 CGTTCTG 280 0.0 40.982143 1 TTAGGCG 55 6.002665E-11 40.909092 1 CGTTTCT 350 0.0 40.5 1 ATCGCCA 50 1.0804797E-9 40.5 24 AACGGGC 90 0.0 40.0 4 TACGCGG 45 1.9270374E-8 40.0 2 ATTCTCG 35 6.2458257E-6 38.57143 13 TACGAAT 65 9.094947E-12 38.076927 12 CACGCGT 30 1.1396011E-4 37.500004 39 >>END_MODULE