SRR3550336_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3975	0.43732644466252696	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2129	0.23423094356893587	RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1918	0.2110168857516294	RNA PCR Primer, Index 41 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1902	0.20925657804984313	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1074	0.11816065448240352	RNA PCR Primer, Index 41 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.032505E-4	45.000004	25
CGTTCGC	20	7.032505E-4	45.000004	17
TAGCCGT	20	7.032505E-4	45.000004	44
ACGATTG	20	7.032505E-4	45.000004	1
GTTAACG	20	7.032505E-4	45.000004	1
CGGTATA	20	7.032505E-4	45.000004	6
TAACGCG	35	1.2115561E-7	45.0	1
AATCGCG	25	3.890159E-5	45.0	1
TAGCGCG	60	0.0	44.999996	1
TATTGCG	30	2.1649994E-6	44.999996	1
TGCGTAG	75	0.0	42.0	1
CGTTTTT	2340	0.0	41.346157	1
TCGTAAG	55	6.002665E-11	40.909092	1
CTACGAA	105	0.0	40.714287	11
GCTACGA	105	0.0	40.714287	10
TTCACGG	50	1.0804797E-9	40.5	2
GCGAACG	45	1.927583E-8	40.000004	1
ATTACGG	45	1.927583E-8	40.000004	2
CGCACGG	125	0.0	39.600002	2
CATGGTC	1210	0.0	39.421486	12