##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908932
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.195834231823724	33.0	31.0	34.0	30.0	34.0
2	32.31037085282507	34.0	31.0	34.0	30.0	34.0
3	32.40079675927352	34.0	31.0	34.0	30.0	34.0
4	35.90514031852768	37.0	35.0	37.0	35.0	37.0
5	35.893974466736786	37.0	35.0	37.0	35.0	37.0
6	35.91534130165953	37.0	35.0	37.0	35.0	37.0
7	36.1300460320464	37.0	35.0	37.0	35.0	37.0
8	36.13201097551852	37.0	36.0	37.0	35.0	37.0
9	37.72575396179252	39.0	38.0	39.0	35.0	39.0
10	37.3978053363728	39.0	37.0	39.0	34.0	39.0
11	37.35321784247886	39.0	37.0	39.0	34.0	39.0
12	37.27756641860997	39.0	37.0	39.0	34.0	39.0
13	37.233729255873925	39.0	37.0	39.0	34.0	39.0
14	38.41057306817232	40.0	38.0	41.0	34.0	41.0
15	38.458925420163446	40.0	38.0	41.0	34.0	41.0
16	38.46849929367654	40.0	38.0	41.0	34.0	41.0
17	38.45155083108527	40.0	38.0	41.0	34.0	41.0
18	38.42378527766654	40.0	38.0	41.0	34.0	41.0
19	38.36376978695876	40.0	37.0	41.0	34.0	41.0
20	38.266680015666736	40.0	37.0	41.0	34.0	41.0
21	38.218410178099134	40.0	37.0	41.0	34.0	41.0
22	38.20147271743101	40.0	37.0	41.0	34.0	41.0
23	38.16285266664613	40.0	37.0	41.0	34.0	41.0
24	38.15266378562973	40.0	37.0	41.0	34.0	41.0
25	38.12420071028416	40.0	37.0	41.0	34.0	41.0
26	38.0180904622128	40.0	37.0	41.0	34.0	41.0
27	37.971331188691785	40.0	36.0	41.0	34.0	41.0
28	37.89415489827622	40.0	36.0	41.0	33.0	41.0
29	37.82451712559355	40.0	36.0	41.0	33.0	41.0
30	37.780556741318385	40.0	36.0	41.0	33.0	41.0
31	37.66032992567101	40.0	36.0	41.0	33.0	41.0
32	37.46379487134351	40.0	36.0	41.0	33.0	41.0
33	37.35378884228963	40.0	36.0	41.0	32.0	41.0
34	37.13209569032667	40.0	35.0	41.0	31.0	41.0
35	37.11247706099026	40.0	35.0	41.0	31.0	41.0
36	37.013433348149256	39.0	35.0	41.0	31.0	41.0
37	36.96992514291498	39.0	35.0	41.0	31.0	41.0
38	36.84349324261881	39.0	35.0	41.0	31.0	41.0
39	36.76884629433225	39.0	35.0	41.0	31.0	41.0
40	36.687039294468676	39.0	35.0	41.0	31.0	41.0
41	36.5995762059208	39.0	35.0	41.0	30.0	41.0
42	36.56007160051577	39.0	35.0	41.0	30.0	41.0
43	36.47558673256085	39.0	35.0	41.0	30.0	41.0
44	36.279528061505154	39.0	35.0	41.0	30.0	41.0
45	36.177788877495786	39.0	35.0	41.0	30.0	41.0
46	36.04329696831006	38.0	35.0	40.0	30.0	41.0
47	35.922285715543076	38.0	35.0	40.0	29.0	41.0
48	35.83408109737582	38.0	35.0	40.0	29.0	41.0
49	35.75909639004898	38.0	35.0	40.0	28.0	41.0
50	35.6652983941593	38.0	35.0	40.0	28.0	41.0
51	34.37934851011957	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	5.0
11	10.0
12	2.0
13	5.0
14	12.0
15	22.0
16	37.0
17	92.0
18	169.0
19	313.0
20	555.0
21	910.0
22	1429.0
23	2353.0
24	3600.0
25	5324.0
26	7838.0
27	9165.0
28	10500.0
29	11873.0
30	14316.0
31	17934.0
32	22228.0
33	30159.0
34	51471.0
35	69053.0
36	68361.0
37	99062.0
38	178985.0
39	302930.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.307455343193986	20.248929512878853	24.913084807224305	12.530530336702855
2	33.21018514036253	23.671077704382725	24.000585302310846	19.118151852943896
3	32.06367473034286	24.615042709465616	26.61552239331435	16.70576016687717
4	27.302812531630526	26.36764906505657	26.540159219831626	19.789379183481273
5	25.022003846272327	30.177064950953426	25.0094616538971	19.791469548877146
6	26.208451237276275	33.0685903896001	24.60811149788983	16.11484687523379
7	82.32959121254395	4.772964314162116	8.465429757121544	4.432014716172387
8	82.70178627224038	3.983466309910973	8.565547257660638	4.749200160188001
9	76.26445102603935	6.687408959086048	10.97705878987647	6.07108122499813
10	34.56815251305928	35.5303807105482	16.62753649337904	13.273930283013472
11	27.315244704774393	25.24589298209327	28.902492155628806	18.53637015750353
12	24.606681247882133	23.521671588193616	30.977014782183925	20.894632381740326
13	23.177421413263037	25.254584501370843	32.22408276966814	19.343911315697984
14	20.076309338872438	27.333287858717704	31.026523436296667	21.5638793661132
15	19.83338687602593	26.11834548679109	33.30975254474482	20.73851509243816
16	23.778896551117136	27.015662337776643	30.225803470446632	18.97963764065959
17	21.99042392610228	25.623148926432343	30.088389450475944	22.298037696989432
18	24.727812421611297	24.16187349548701	29.591322563184047	21.51899151971765
19	22.468787544062703	27.779635880351883	27.605915514031853	22.14566106155356
20	25.924931678057323	27.41954293610523	27.238891358209415	19.416634027628028
21	24.0577953026189	28.248647863646564	28.117945016788937	19.5756118169456
22	22.72326202620218	24.182337072520276	28.437110806969056	24.657290094308486
23	21.42811563461293	28.330172114085546	29.066420810357652	21.175291440943877
24	22.573635871550344	25.12157125065461	30.018087161635854	22.286705716159187
25	21.99284434919224	27.324266281746052	27.90648805411186	22.776401314949855
26	21.234591806647803	26.855584356145457	28.642736750383968	23.267087086822777
27	22.365699524276845	25.451078848582732	28.077127881953768	24.10609374518666
28	19.471973701002934	28.20177967108651	28.6128115194536	23.71343510845696
29	21.331958826402854	26.6495183358051	28.175595094022437	23.84292774376961
30	23.68295978136978	23.73631910858018	29.340808773373585	23.23991233667645
31	23.87186280161772	26.618822970255202	26.37634058433414	23.132973643792933
32	22.47692896718346	26.766028701817078	27.63727099496992	23.119771336029537
33	21.20587678726241	25.70038242684821	25.975980601409127	27.11776018448025
34	20.073668877319754	27.628799514155073	29.631149524936962	22.66638208358821
35	18.542091157534337	27.76665361105121	29.73390748702873	23.957347744385718
36	19.967830376749856	29.229469311235608	26.341134430298418	24.461565881716123
37	20.818939150563516	28.539978788292192	26.257849872157653	24.383232188986632
38	20.33782505181906	28.825698732138378	27.064070799575767	23.7724054164668
39	22.080859734281553	25.543715041389238	25.993803716889712	26.3816215074395
40	22.606201564033395	24.68171436367077	29.468651120215817	23.243432952080024
41	18.849704928421488	26.391303199799328	28.318290037098485	26.440701834680702
42	22.042132964842253	25.371754982770987	27.780295995740055	24.8058160566467
43	22.00351621463432	24.295546861591404	27.63243014879001	26.068506774984268
44	22.321361774038103	25.36669409812835	27.00697081849907	25.30497330933447
45	22.50696421734519	23.601765588624893	27.39643889751929	26.49483129651063
46	22.3024384662439	24.873697922396833	26.99508874151202	25.828774869847248
47	19.615548797929875	26.04793317871964	29.970118776762178	24.3663992465883
48	21.02621538244885	24.591718632416946	29.28480898461051	25.097257000523694
49	22.74933658403489	23.911579744139274	29.081603464285553	24.257480207540276
50	19.791469548877146	24.4197585737987	29.427723966149284	26.36104791117487
51	20.2238451281284	22.76330902641782	28.024098612437452	28.988747233016333
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	298.0
1	604.0
2	910.0
3	3831.5
4	6753.0
5	4450.0
6	2147.0
7	2127.5
8	2108.0
9	2155.0
10	2202.0
11	2238.5
12	2275.0
13	2278.0
14	2281.0
15	2262.0
16	2243.0
17	2237.5
18	2232.0
19	2327.0
20	2422.0
21	2811.5
22	3201.0
23	3278.0
24	3355.0
25	3994.0
26	5580.0
27	6527.0
28	8132.5
29	9738.0
30	11015.5
31	12293.0
32	15308.0
33	18323.0
34	20584.5
35	22846.0
36	24301.5
37	25757.0
38	27922.5
39	30088.0
40	33613.0
41	37138.0
42	41864.0
43	46590.0
44	52599.5
45	58609.0
46	69332.0
47	80055.0
48	89209.5
49	98364.0
50	98012.5
51	97661.0
52	88741.5
53	79822.0
54	71093.0
55	62364.0
56	55244.5
57	48125.0
58	42558.5
59	36992.0
60	34056.5
61	31121.0
62	27408.5
63	23696.0
64	19797.5
65	15899.0
66	12760.0
67	9621.0
68	8094.5
69	6568.0
70	5274.0
71	3980.0
72	3548.0
73	3116.0
74	3054.0
75	2471.5
76	1951.0
77	1457.5
78	964.0
79	672.0
80	380.0
81	276.5
82	173.0
83	109.0
84	45.0
85	45.5
86	46.0
87	29.0
88	12.0
89	8.5
90	5.0
91	5.0
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	908932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.27056644354367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.51885830543164	24.852623519662234
2	10.27978794228294	7.045883114030932
3	3.7256468065631334	3.830400792884945
4	1.9602260304113188	2.6871222567829984
5	1.2830518084676978	2.1985456126300553
6	0.9713739654848567	1.9973721615406363
7	0.7900702879493569	1.895330940876668
8	0.7286272872028664	1.9976375886931848
9	0.6289731053020683	1.9399738136810871
>10	6.969048236236628	44.4099838761691
>50	0.09310979585427084	2.194892347936635
>100	0.04607156805224122	2.930789370466526
>500	0.003543966773233479	0.8051279418847266
>1k	0.0016108939878333995	1.2143166627603157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3975	0.43732644466252696	No Hit
CCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	2129	0.23423094356893587	RNA PCR Primer, Index 41 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGCT	1918	0.2110168857516294	RNA PCR Primer, Index 41 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTGC	1902	0.20925657804984313	Illumina PCR Primer Index 11 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCTCGACATCTCGTATGCCGTCTTCTG	1074	0.11816065448240352	RNA PCR Primer, Index 41 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1001923136164202E-4	0.0	0.0	0.2330207320239578	0.0
2	1.1001923136164202E-4	0.0	0.0	0.8789436393481581	0.0
3	1.1001923136164202E-4	0.0	0.0	1.30988896859171	0.0
4	1.1001923136164202E-4	0.0	0.0	1.8322602790967861	0.0
5	1.1001923136164202E-4	0.0	0.0	3.296836286982965	0.0
6	1.1001923136164202E-4	0.0	0.0	4.403519735249722	0.0
7	1.1001923136164202E-4	0.0	0.0	5.424718240748483	0.0
8	1.1001923136164202E-4	0.0	0.0	7.0615843649469925	0.0
9	1.1001923136164202E-4	0.0	0.0	7.780560041895323	0.0
10	2.2003846272328404E-4	0.0	0.0	9.087038414314822	0.0
11	2.2003846272328404E-4	0.0	0.0	10.708501846122703	0.0
12	2.2003846272328404E-4	0.0	0.0	12.01365998776586	0.0
13	2.2003846272328404E-4	0.0	0.0	12.605233394797411	0.0
14	2.2003846272328404E-4	0.0	0.0	12.862348338489568	0.0
15	2.2003846272328404E-4	0.0	0.0	13.288782879247293	0.0
16	2.2003846272328404E-4	0.0	0.0	14.035923479424204	0.0
17	2.2003846272328404E-4	0.0	0.0	14.993640888427297	0.0
18	3.3005769408492606E-4	0.0	0.0	15.98018333604714	0.0
19	3.3005769408492606E-4	0.0	0.0	16.606852877883053	0.0
20	3.3005769408492606E-4	0.0	0.0	17.244744381317854	0.0
21	3.3005769408492606E-4	0.0	0.0	17.987264173777575	0.0
22	3.3005769408492606E-4	0.0	0.0	18.758278947159962	0.0
23	3.3005769408492606E-4	0.0	0.0	19.55338793221055	0.0
24	3.3005769408492606E-4	0.0	0.0	20.148701993108396	0.0
25	3.3005769408492606E-4	0.0	0.0	20.688896419094057	0.0
26	3.3005769408492606E-4	0.0	0.0	21.194654825663527	0.0
27	3.3005769408492606E-4	0.0	0.0	21.741340386299527	0.0
28	3.3005769408492606E-4	0.0	0.0	22.285935581539654	0.0
29	3.3005769408492606E-4	0.0	0.0	22.855725180761596	0.0
30	3.3005769408492606E-4	0.0	0.0	23.562488723028785	0.0
31	4.400769254465681E-4	0.0	0.0	24.160223207016585	0.0
32	4.400769254465681E-4	0.0	0.0	24.765218960274257	0.0
33	5.500961568082101E-4	0.0	0.0	25.35041125188683	0.0
34	5.500961568082101E-4	0.0	0.0	25.922841312661454	0.0
35	5.500961568082101E-4	0.0	0.0	26.497801815757395	0.0
36	5.500961568082101E-4	0.0	0.0	27.063410684187595	0.0
37	5.500961568082101E-4	0.0	0.0	27.618127648712996	0.0
38	5.500961568082101E-4	0.0	0.0	28.212671574991308	0.0
39	5.500961568082101E-4	0.0	0.0	28.85331355921015	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.032505E-4	45.000004	25
CGTTCGC	20	7.032505E-4	45.000004	17
TAGCCGT	20	7.032505E-4	45.000004	44
ACGATTG	20	7.032505E-4	45.000004	1
GTTAACG	20	7.032505E-4	45.000004	1
CGGTATA	20	7.032505E-4	45.000004	6
TAACGCG	35	1.2115561E-7	45.0	1
AATCGCG	25	3.890159E-5	45.0	1
TAGCGCG	60	0.0	44.999996	1
TATTGCG	30	2.1649994E-6	44.999996	1
TGCGTAG	75	0.0	42.0	1
CGTTTTT	2340	0.0	41.346157	1
TCGTAAG	55	6.002665E-11	40.909092	1
CTACGAA	105	0.0	40.714287	11
GCTACGA	105	0.0	40.714287	10
TTCACGG	50	1.0804797E-9	40.5	2
GCGAACG	45	1.927583E-8	40.000004	1
ATTACGG	45	1.927583E-8	40.000004	2
CGCACGG	125	0.0	39.600002	2
CATGGTC	1210	0.0	39.421486	12
>>END_MODULE