FastQCFastQC Report
Sat 18 Jun 2016
SRR3550335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences615518
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC18170.2951985157217173No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC14330.23281203799076552No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT13610.22111457341621202No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9900.16084013790011015No Hit
TCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGC6820.11080098388674255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAT207.0307293E-445.00000412
GTCTAGT207.0307293E-445.00000410
AGTGCGT207.0307293E-445.00000433
TCGACAA351.2107012E-745.00000419
CTCACGT253.8886872E-545.045
CGCATCG253.8886872E-545.021
CGAATAT502.1827873E-1145.014
GTACCCT302.1638516E-644.99999615
CCCTATA302.1638516E-644.9999966
AATACGG302.1638516E-644.9999962
CGCGAGG1250.041.42
CGACAAT556.002665E-1140.90909220
GCTACGA556.002665E-1140.90909210
TAATGCG556.002665E-1140.90909224
TATTGCG403.4547338E-739.3750041
GAATACG356.243561E-638.571431
CATCGTT356.243561E-638.5714336
TGGGATC1600.037.9687546
CGTTTTT6650.037.5563931
CGAGACA3000.037.522