FastQCFastQC Report
Sat 18 Jun 2016
SRR3550333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622927
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85521.3728735469806252No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTT21100.3387234780319363No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG20530.32957312815145273No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCT15070.2419224082436626No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTC14530.2332536557253097No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC11290.18124114061519248No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT9540.15314796115756743Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC8120.13035235268338022No Hit
CGTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCT8090.12987075532124953No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTC6580.10563035476067019No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAT207.0307957E-445.011
GCAACGA351.2107375E-745.011
TAACGGT207.0307957E-445.03
ACTAGCG207.0307957E-445.01
GTATGCG207.0307957E-445.01
CGAATTA207.0307957E-445.041
GTATACG351.2107375E-745.01
AAATGCG207.0307957E-445.01
CGTTTTT59050.043.6663861
CTAGCGG700.041.7857132
GCGTAAG501.0804797E-940.51
ACAACGA950.040.26315713
CGTATGG451.925946E-839.9999962
CGAATAT900.039.99999614
CGCGCAA403.4548248E-739.37518
GCGTTAG403.4548248E-739.3751
GCTAGCG403.4548248E-739.3751
ATAACGG403.4548248E-739.3752
CGCAATC403.4548248E-739.37520
TTTAGCG356.243683E-638.5714261