FastQCFastQC Report
Sat 18 Jun 2016
SRR3550331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711031
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.42515727162388134No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC8810.12390458362574908No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC8200.1153254921374736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA253.8892995E-545.024
TAGGTCG207.031469E-445.01
GATCACG253.8892995E-545.01
CGCTATG207.031469E-445.016
ACGCTAT207.031469E-445.015
GCGATAA556.002665E-1140.909099
CGAATAT1400.040.1785714
CGTTTTT15650.039.824281
ACGGTAG403.4557343E-739.3751
GCTACGA1450.038.79310210
ATTGGAC356.244929E-638.57142612
CGCACGG356.244929E-638.5714262
TTCGAAG356.244929E-638.5714261
TACGTAG356.244929E-638.5714261
TAGGGAT5550.038.108115
GCGAGAC1850.037.70270521
CTATACG301.1394837E-437.5000041
TTACGAG301.1394837E-437.5000041
ACACGAC1800.037.526
GTACGAG850.037.0588231
Produced by FastQC (version 0.11.5)