##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550329_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	888800
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34063906390639	33.0	31.0	34.0	30.0	34.0
2	32.45374549954995	34.0	31.0	34.0	31.0	34.0
3	32.52669104410441	34.0	31.0	34.0	31.0	34.0
4	36.02244149414941	37.0	35.0	37.0	35.0	37.0
5	35.99464221422142	37.0	35.0	37.0	35.0	37.0
6	36.01043204320432	37.0	35.0	37.0	35.0	37.0
7	36.17571332133213	37.0	36.0	37.0	35.0	37.0
8	36.154834608460845	37.0	36.0	37.0	35.0	37.0
9	37.748023177317734	39.0	38.0	39.0	35.0	39.0
10	37.513764626462645	39.0	37.0	39.0	35.0	39.0
11	37.46190256525652	39.0	37.0	39.0	35.0	39.0
12	37.33175517551755	39.0	37.0	39.0	35.0	39.0
13	37.278395589558954	39.0	37.0	39.0	34.0	39.0
14	38.509501575157515	40.0	38.0	41.0	34.0	41.0
15	38.562946669666964	40.0	38.0	41.0	34.0	41.0
16	38.591489648964895	40.0	38.0	41.0	34.0	41.0
17	38.56664828982898	40.0	38.0	41.0	34.0	41.0
18	38.500363411341134	40.0	38.0	41.0	34.0	41.0
19	38.433207695769575	40.0	37.0	41.0	34.0	41.0
20	38.307731773177316	40.0	37.0	41.0	34.0	41.0
21	38.25534203420342	40.0	37.0	41.0	34.0	41.0
22	38.232676642664266	40.0	37.0	41.0	34.0	41.0
23	38.18491449144914	40.0	37.0	41.0	34.0	41.0
24	38.16662128712871	40.0	37.0	41.0	34.0	41.0
25	38.12038816381638	40.0	36.0	41.0	34.0	41.0
26	37.99021939693969	40.0	36.0	41.0	34.0	41.0
27	37.934492574257426	40.0	36.0	41.0	34.0	41.0
28	37.823680243024306	40.0	36.0	41.0	33.0	41.0
29	37.745958595859584	40.0	36.0	41.0	33.0	41.0
30	37.703791629162914	40.0	36.0	41.0	33.0	41.0
31	37.58301755175518	40.0	35.0	41.0	33.0	41.0
32	37.38736723672367	40.0	35.0	41.0	33.0	41.0
33	37.23166179117912	40.0	35.0	41.0	32.0	41.0
34	37.004277677767774	39.0	35.0	41.0	31.0	41.0
35	36.95543204320432	39.0	35.0	41.0	31.0	41.0
36	36.797542754275426	39.0	35.0	41.0	31.0	41.0
37	36.75942619261926	39.0	35.0	41.0	31.0	41.0
38	36.60655828082808	39.0	35.0	41.0	31.0	41.0
39	36.5288501350135	39.0	35.0	41.0	30.0	41.0
40	36.406110486048604	39.0	35.0	41.0	30.0	41.0
41	36.271696669666966	39.0	35.0	41.0	30.0	41.0
42	36.2082200720072	39.0	35.0	41.0	30.0	41.0
43	36.1263805130513	39.0	35.0	41.0	30.0	41.0
44	35.931686543654365	38.0	35.0	41.0	29.0	41.0
45	35.84671467146715	38.0	35.0	40.0	29.0	41.0
46	35.72033303330333	38.0	35.0	40.0	29.0	41.0
47	35.607709270927096	38.0	35.0	40.0	29.0	41.0
48	35.48516089108911	38.0	35.0	40.0	28.0	41.0
49	35.389499324932494	37.0	35.0	40.0	28.0	41.0
50	35.29799842484248	37.0	34.0	40.0	28.0	41.0
51	34.02407403240324	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	8.0
12	9.0
13	8.0
14	16.0
15	21.0
16	58.0
17	119.0
18	218.0
19	369.0
20	656.0
21	1048.0
22	1578.0
23	2514.0
24	3687.0
25	5512.0
26	7961.0
27	9743.0
28	10751.0
29	12224.0
30	14393.0
31	17068.0
32	21643.0
33	29413.0
34	52790.0
35	69791.0
36	68107.0
37	98359.0
38	170697.0
39	289844.0
40	185.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.125	19.522839783978398	23.422142214221424	12.930018001800182
2	34.41775427542754	23.584945994599458	23.39502700270027	18.602272727272727
3	33.00607560756076	24.285666066606662	26.123312331233123	16.584945994599458
4	27.84068406840684	25.84529702970297	26.451057605760575	19.862961296129615
5	25.937781278127815	29.839671467146715	24.085508550855085	20.137038703870385
6	26.806143114311432	33.923154815481546	23.83730873087309	15.433393339333934
7	81.0569306930693	5.582695769576958	8.934068406840684	4.426305130513051
8	81.33449594959497	5.154252925292529	9.057943294329434	4.4533078307830785
9	74.72445994599461	7.036566156615661	11.31075607560756	6.928217821782178
10	39.44261926192619	27.44869486948695	17.669554455445542	15.439131413141313
11	31.73436093609361	25.332808280828083	23.94228172817282	18.99054905490549
12	28.04072907290729	23.355985598559855	27.56683168316832	21.036453645364535
13	25.14457695769577	23.871287128712872	29.521377137713774	21.46275877587759
14	21.38973897389739	26.563231323132314	29.50461296129613	22.542416741674167
15	21.1993699369937	24.74122412241224	32.18406840684068	21.875337533753374
16	24.648064806480647	24.37736273627363	30.721984698469846	20.252587758775878
17	22.780265526552657	24.271827182718273	28.98526102610261	23.962646264626464
18	25.582133213321335	23.29027902790279	28.139176417641764	22.988411341134114
19	25.083033303330332	26.464896489648964	26.095972097209717	22.356098109810983
20	28.747299729972998	24.646714671467144	26.088208820882087	20.517776777677767
21	26.155265526552657	26.636138613861387	26.45049504950495	20.75810081008101
22	25.374662466246622	23.407065706570656	26.57133213321332	24.646939693969397
23	24.288591359135914	27.038141314131416	26.707808280828083	21.965459045904588
24	24.116449144914494	23.616786678667868	28.767439243924393	23.49932493249325
25	24.46804680468047	25.674842484248423	26.25855085508551	23.5985598559856
26	23.584158415841582	25.804342934293427	27.275090009000902	23.336408640864086
27	24.037691269126913	25.20724572457246	26.885801080108013	23.86926192619262
28	22.099797479747973	27.729297929792978	27.12173717371737	23.049167416741675
29	23.646039603960396	25.798604860486048	26.988861386138613	23.56649414941494
30	24.610823582358236	23.194419441944195	28.779477947794778	23.41527902790279
31	24.979410441044102	25.820319531953196	25.399077407740773	23.801192619261926
32	24.922929792979296	25.520027002700267	27.39615211521152	22.16089108910891
33	24.280715571557156	24.78724122412241	26.14187668766877	24.790166516651666
34	23.509788478847884	26.53712871287129	28.455108010801077	21.49797479747975
35	23.61858685868587	25.19250675067507	28.230760576057605	22.958145814581457
36	23.13670117011701	28.52857785778578	25.925067506750676	22.409653465346537
37	25.28611611161116	26.68136813681368	25.693069306930695	22.339446444644466
38	24.342484248424842	26.46962196219622	26.670004500450045	22.517889288928895
39	26.342259225922593	23.457358235823584	26.238411341134118	23.96197119711971
40	26.189356435643564	23.131750675067504	29.482448244824482	21.196444644464446
41	22.566831683168317	25.60879837983798	28.018564356435643	23.805805580558058
42	24.98064806480648	25.259563456345635	26.78870387038704	22.971084608460846
43	24.614311431143115	24.37083708370837	27.195882088208823	23.818969396939693
44	24.893676867686768	24.882313231323135	26.498199819982	23.7258100810081
45	24.843159315931594	22.87567506750675	26.980873087308733	25.30029252925293
46	23.481435643564357	24.28319081908191	27.08134563456346	25.154027902790276
47	23.448244824482448	25.38580108010801	28.614761476147617	22.551192619261926
48	23.935418541854187	24.249887488748875	28.225810081008103	23.58888388838884
49	24.322344734473447	24.136363636363637	27.541179117911792	24.000112511251125
50	21.911678667866788	24.51473897389739	28.641989198919894	24.93159315931593
51	22.052767776777678	23.238748874887488	27.54027902790279	27.168204320432043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	315.0
1	775.5
2	1236.0
3	4868.5
4	8501.0
5	5616.0
6	2731.0
7	2732.0
8	2733.0
9	2686.0
10	2639.0
11	2721.0
12	2803.0
13	2669.0
14	2535.0
15	2430.0
16	2325.0
17	2378.5
18	2432.0
19	2409.0
20	2386.0
21	2460.5
22	2535.0
23	2767.5
24	3000.0
25	3788.5
26	4671.5
27	4766.0
28	6371.0
29	7976.0
30	8979.0
31	9982.0
32	11598.0
33	13214.0
34	15808.5
35	18403.0
36	19766.0
37	21129.0
38	23443.0
39	25757.0
40	29133.0
41	32509.0
42	36101.0
43	39693.0
44	44016.0
45	48339.0
46	55727.0
47	63115.0
48	69677.0
49	76239.0
50	78221.0
51	80203.0
52	75463.0
53	70723.0
54	65277.5
55	59832.0
56	56374.5
57	52917.0
58	50125.5
59	47334.0
60	46926.5
61	46519.0
62	41862.5
63	37206.0
64	32953.5
65	28701.0
66	24370.5
67	20040.0
68	16703.5
69	13367.0
70	11464.5
71	9562.0
72	8412.5
73	7263.0
74	6418.0
75	4644.5
76	3716.0
77	2900.5
78	2085.0
79	1432.5
80	780.0
81	648.5
82	517.0
83	399.0
84	281.0
85	231.5
86	182.0
87	125.0
88	68.0
89	55.5
90	43.0
91	26.5
92	10.0
93	7.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	888800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.48823532990829
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40460973139533	22.798933901923434
2	8.757387630737625	5.515093651837886
3	3.255568773038568	3.0753634697441767
4	1.831938236978296	2.307380092633195
5	1.3290288803988435	2.0924387073121657
6	1.0834874968166746	2.0470265566066024
7	0.950920271948314	2.0959960912162225
8	0.8267429968596341	2.082614243397583
9	0.7497616828251038	2.1247805079130435
>10	8.66594824930639	49.73736422737661
>50	0.09724129847882977	2.051285021612095
>100	0.04270004086935867	2.4052737595571543
>500	0.003588238728516435	0.8337333273097097
>1k	7.176477457032872E-4	0.2671019902100764
>5k	3.588238728516436E-4	0.5656144513501026
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5006	0.5632313231323133	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTG	1236	0.13906390639063906	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	1128	0.1269126912691269	TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTTCTGC	941	0.10587308730873088	TruSeq Adapter, Index 14 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCAGTGTGTCGTATGCCGTCTT	934	0.10508550855085508	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.25022502250225E-4	0.0	0.0	0.11014851485148515	0.0
2	2.25022502250225E-4	0.0	0.0	0.4362061206120612	0.0
3	2.25022502250225E-4	0.0	0.0	0.7507875787578758	0.0
4	2.25022502250225E-4	0.0	0.0	1.0471422142214222	0.0
5	2.25022502250225E-4	0.0	0.0	1.9564581458145816	0.0
6	2.25022502250225E-4	0.0	0.0	2.80479297929793	0.0
7	2.25022502250225E-4	0.0	0.0	3.50022502250225	0.0
8	2.25022502250225E-4	0.0	0.0	4.59462196219622	0.0
9	2.25022502250225E-4	0.0	0.0	5.156840684068407	0.0
10	2.25022502250225E-4	0.0	0.0	6.126800180018002	0.0
11	2.25022502250225E-4	0.0	0.0	7.192731773177318	0.0
12	2.25022502250225E-4	0.0	0.0	8.071894689468946	0.0
13	2.25022502250225E-4	0.0	0.0	8.463996399639964	0.0
14	3.3753375337533753E-4	0.0	0.0	8.67034203420342	0.0
15	3.3753375337533753E-4	0.0	0.0	8.97052205220522	0.0
16	3.3753375337533753E-4	0.0	0.0	9.507313231323133	0.0
17	3.3753375337533753E-4	0.0	0.0	10.082808280828083	0.0
18	3.3753375337533753E-4	0.0	0.0	10.702295229522953	0.0
19	3.3753375337533753E-4	0.0	0.0	11.106773177317733	0.0
20	4.5004500450045E-4	0.0	0.0	11.510013501350135	0.0
21	4.5004500450045E-4	0.0	0.0	12.0	0.0
22	4.5004500450045E-4	0.0	0.0	12.51451395139514	0.0
23	4.5004500450045E-4	0.0	0.0	13.028127812781278	0.0
24	4.5004500450045E-4	0.0	0.0	13.462983798379838	0.0
25	4.5004500450045E-4	0.0	0.0	13.85564806480648	0.0
26	4.5004500450045E-4	0.0	0.0	14.224684968496849	0.0
27	4.5004500450045E-4	0.0	0.0	14.585733573357336	0.0
28	4.5004500450045E-4	0.0	0.0	14.960621062106211	0.0
29	4.5004500450045E-4	0.0	0.0	15.352385238523853	0.0
30	4.5004500450045E-4	0.0	0.0	15.888388838883888	0.0
31	4.5004500450045E-4	0.0	0.0	16.32155715571557	0.0
32	4.5004500450045E-4	0.0	0.0	16.802317731773176	0.0
33	4.5004500450045E-4	0.0	0.0	17.216471647164717	0.0
34	4.5004500450045E-4	0.0	0.0	17.610373537353734	0.0
35	4.5004500450045E-4	0.0	0.0	18.035328532853285	0.0
36	4.5004500450045E-4	0.0	0.0	18.450607560756076	0.0
37	5.625562556255626E-4	0.0	0.0	18.894801980198018	0.0
38	6.750675067506751E-4	0.0	0.0	19.344284428442844	0.0
39	6.750675067506751E-4	0.0	0.0	19.81514401440144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.0324226E-4	45.0	41
GCGATCG	25	3.8900896E-5	45.0	9
TATCGAG	20	7.0324226E-4	45.0	1
CCGTTAG	25	3.8900896E-5	45.0	1
CGAATAT	130	0.0	41.538464	14
CGTTTTT	2795	0.0	41.37746	1
AACGGGA	265	0.0	40.75472	4
TTAAGCG	45	1.9274012E-8	40.0	1
GTACTAG	45	1.9274012E-8	40.0	1
TAGGACG	45	1.9274012E-8	40.0	1
TCGTTTA	40	3.457044E-7	39.375	38
AAGGGCG	310	0.0	38.46774	5
GCTACGA	135	0.0	38.333336	10
GCAAACG	90	0.0	37.5	1
GTCGATG	30	1.1397137E-4	37.499996	1
CGTATAT	30	1.1397137E-4	37.499996	14
ACGTTAG	30	1.1397137E-4	37.499996	1
ATAGACG	30	1.1397137E-4	37.499996	1
AATTGCG	30	1.1397137E-4	37.499996	1
TCCGTAT	30	1.1397137E-4	37.499996	12
>>END_MODULE