##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	875475
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351517747508495	33.0	31.0	34.0	30.0	34.0
2	32.4996476198635	34.0	31.0	34.0	31.0	34.0
3	32.48015077529341	34.0	31.0	34.0	31.0	34.0
4	36.02984094348782	37.0	35.0	37.0	35.0	37.0
5	36.013074045518145	37.0	35.0	37.0	35.0	37.0
6	36.039952026043004	37.0	35.0	37.0	35.0	37.0
7	36.20174191153374	37.0	36.0	37.0	35.0	37.0
8	36.208442274194006	37.0	37.0	37.0	35.0	37.0
9	37.830827836317425	39.0	38.0	39.0	35.0	39.0
10	37.59840429481139	39.0	37.0	39.0	35.0	39.0
11	37.57596733201976	39.0	37.0	39.0	35.0	39.0
12	37.51038921728205	39.0	37.0	39.0	35.0	39.0
13	37.47841114823382	39.0	37.0	39.0	35.0	39.0
14	38.73346925954482	40.0	38.0	41.0	35.0	41.0
15	38.788872326451354	40.0	38.0	41.0	35.0	41.0
16	38.80468431422942	40.0	38.0	41.0	35.0	41.0
17	38.81097232930695	40.0	38.0	41.0	35.0	41.0
18	38.61210885519289	40.0	38.0	41.0	35.0	41.0
19	38.41342585453611	40.0	37.0	41.0	35.0	41.0
20	38.153753105457035	40.0	36.0	41.0	34.0	41.0
21	38.0931277306605	40.0	35.0	41.0	34.0	41.0
22	38.08229703875039	40.0	35.0	41.0	34.0	41.0
23	38.02935663496959	40.0	35.0	41.0	34.0	41.0
24	38.002862446100686	40.0	35.0	41.0	34.0	41.0
25	37.963960135926214	40.0	35.0	41.0	34.0	41.0
26	37.85460235871955	40.0	35.0	41.0	34.0	41.0
27	37.80718352894143	40.0	35.0	41.0	34.0	41.0
28	37.69610668494246	40.0	35.0	41.0	33.0	41.0
29	37.61863102887004	40.0	35.0	41.0	33.0	41.0
30	37.50948113881036	40.0	35.0	41.0	33.0	41.0
31	37.288146434792544	40.0	35.0	41.0	33.0	41.0
32	36.91774865073246	40.0	35.0	41.0	31.0	41.0
33	36.520178188983124	39.0	35.0	41.0	30.0	41.0
34	36.105883092035754	39.0	35.0	41.0	28.0	41.0
35	35.90887575316257	39.0	35.0	41.0	26.0	41.0
36	35.676877123847056	39.0	35.0	41.0	25.0	41.0
37	35.60874154030669	39.0	35.0	41.0	24.0	41.0
38	35.459991433221965	39.0	35.0	41.0	24.0	41.0
39	35.34448042491219	39.0	35.0	41.0	23.0	41.0
40	35.24080527713527	39.0	35.0	41.0	23.0	41.0
41	35.06811388103601	39.0	35.0	41.0	22.0	41.0
42	34.9807304606071	39.0	35.0	41.0	22.0	41.0
43	34.90838687569605	39.0	35.0	41.0	21.0	41.0
44	34.72080870384649	38.0	34.0	41.0	20.0	41.0
45	34.64064764841943	38.0	34.0	41.0	20.0	41.0
46	34.57921813872469	38.0	34.0	40.0	20.0	41.0
47	34.505298266655245	38.0	34.0	40.0	20.0	41.0
48	34.37023558639596	38.0	34.0	40.0	20.0	41.0
49	34.28981752762786	38.0	34.0	40.0	20.0	41.0
50	34.21134584082927	38.0	34.0	40.0	20.0	41.0
51	33.0598109597647	36.0	32.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	13.0
12	6.0
13	6.0
14	15.0
15	27.0
16	50.0
17	114.0
18	246.0
19	471.0
20	801.0
21	1328.0
22	2073.0
23	3184.0
24	5655.0
25	10396.0
26	16338.0
27	19319.0
28	18823.0
29	17261.0
30	16788.0
31	17993.0
32	20943.0
33	26861.0
34	44356.0
35	56913.0
36	60290.0
37	85069.0
38	153585.0
39	296284.0
40	261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.86313144293098	18.42828178988549	21.647562751649104	21.061024015534425
2	40.583683143436424	21.532196807447384	21.774579513978125	16.109540535138066
3	29.208029926611268	22.19126759758988	33.08809503412433	15.51260744167452
4	25.334932465233155	23.97464233701705	33.00117079299809	17.689254404751704
5	23.807076158656727	27.430480596247754	31.226477055312827	17.53596618978269
6	24.342899568805507	31.425112082012625	29.95311116822296	14.278877180958908
7	74.99117621862418	4.659299237556755	16.117307747222938	4.232216796596133
8	76.11239612781633	4.012793055198607	15.896627545046973	3.9781832719380903
9	69.48936291727348	6.439589936891402	18.32113995259716	5.749907193237957
10	32.246380536280306	30.11393814786259	23.947114423598617	13.692566892258487
11	24.926811159656186	22.786144664325082	35.12401839001685	17.163025786001885
12	22.534852508638167	21.29392615437334	36.60641366115537	19.56480767583312
13	21.208029926611268	21.49313229960878	38.75998743539222	18.538850338387732
14	19.07261772180816	23.935235158057054	37.60609954596077	19.38604757417402
15	18.833890173905594	23.677432251063706	38.47237214083783	19.01630543419287
16	21.88286358833776	23.7378565921357	36.58379736714355	17.79548245238299
17	21.474056940518004	23.415517290613668	35.38684714012393	19.723578628744395
18	22.223478683000657	22.76135811987778	35.25720323253091	19.75795996459065
19	20.9407464519261	25.522030897512778	33.726148662154834	19.811073988406296
20	22.58225534709729	25.029041377537908	33.13184271395529	19.25686056140952
21	21.838316342556897	25.242753933578914	34.03535223735686	18.883577486507324
22	21.57777206659242	22.260487164110913	34.42257060452897	21.7391701647677
23	19.953282503783658	25.10145920785859	34.47705531283018	20.46820297552757
24	19.814386475913075	23.783546074987864	35.74356777749222	20.65849967160684
25	21.619806390816414	24.851994631485766	33.346126388531935	20.18207258916588
26	20.56403666580999	25.284902481510034	33.24286815728604	20.90819269539393
27	21.124989291527456	23.429452582883577	33.93026642679688	21.515291698792087
28	19.9399183300494	25.2757645849396	33.70261857848597	21.08169850652503
29	20.745423912733088	23.946657528770096	33.19352351580571	22.11439504269111
30	21.32590879236986	23.272966104114907	34.38487678117593	21.0162483223393
31	21.31322996087838	24.859990290984893	32.37716668094463	21.449613067192097
32	21.223221679659613	24.14620634512693	32.49184728290357	22.13872469230989
33	20.048316628116165	25.862417544761417	31.070219023958423	23.019046803164
34	19.922784773979842	26.746394814243697	31.836545875096377	21.494274536680088
35	20.656329421171364	26.753705131500045	30.78591621691082	21.804049230417775
36	20.287615294554385	29.06376538450555	29.530026557011908	21.118592763928152
37	21.457494502984094	27.597818327193806	29.47714098061053	21.46754618921157
38	20.609040806419372	27.273194551529166	30.91259030811845	21.20517433393301
39	21.518604186298866	26.746394814243697	29.4064365058968	22.328564493560638
40	22.760215882806474	25.5356235186613	31.55087238356321	20.153288214969017
41	20.480196464776267	26.740226734058652	30.900939489991146	21.878637311173936
42	21.477255204317654	26.848510808418286	30.424398183843053	21.249835803421
43	21.30272137982238	25.757103286787174	31.183871612553187	21.75630372083726
44	22.235929067077873	25.725463319912045	29.892915274565233	22.145692338444846
45	21.58085610668494	24.700419772123706	30.05008709557669	23.668637025614668
46	21.29712441817299	25.42836745766584	29.670064821953794	23.604443302207372
47	21.22447814043805	25.578571632542335	30.970044832805048	22.22690539421457
48	20.837716668094465	25.285473600045687	31.137039892629716	22.73976983923013
49	21.575716039864073	24.622747651275024	30.700476883977267	23.101059424883637
50	19.84328507381707	24.53422427824895	32.30394928467403	23.318541363259946
51	20.347582740797854	24.11856420800137	31.37268340043976	24.161169650761018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	598.0
2	860.0
3	13775.0
4	26690.0
5	17774.0
6	8858.0
7	8614.5
8	8371.0
9	8297.5
10	8224.0
11	8082.5
12	7941.0
13	7662.5
14	7384.0
15	6945.5
16	6507.0
17	6159.0
18	5811.0
19	5474.5
20	5138.0
21	5156.0
22	5174.0
23	5157.5
24	5141.0
25	5833.0
26	7206.0
27	7887.0
28	9024.5
29	10162.0
30	11975.5
31	13789.0
32	15294.0
33	16799.0
34	19185.5
35	21572.0
36	23812.0
37	26052.0
38	28431.5
39	30811.0
40	33659.0
41	36507.0
42	41295.0
43	46083.0
44	54644.0
45	63205.0
46	68190.5
47	73176.0
48	74496.5
49	75817.0
50	69578.0
51	63339.0
52	58341.0
53	53343.0
54	50150.0
55	46957.0
56	43819.5
57	40682.0
58	37981.0
59	35280.0
60	32736.5
61	30193.0
62	27357.5
63	24522.0
64	21222.5
65	17923.0
66	14910.0
67	11897.0
68	10253.0
69	8609.0
70	7443.0
71	6277.0
72	5448.0
73	4619.0
74	3835.5
75	2515.5
76	1979.0
77	1540.0
78	1101.0
79	742.0
80	383.0
81	310.5
82	238.0
83	194.0
84	150.0
85	106.0
86	62.0
87	43.0
88	24.0
89	19.5
90	15.0
91	8.5
92	2.0
93	3.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	875475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.722601082073886
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8128546487488	27.420579220463807
2	8.435570841042496	6.532944891545261
3	4.244244723518091	4.93044585970464
4	2.932643450345652	4.542383297747658
5	2.2638915470273098	4.383188463430882
6	1.9470243194866628	4.523630761234706
7	1.5794996197207103	4.281363357861272
8	1.3248290854752067	4.104066254302816
9	1.145177561630696	3.9909828498450777
>10	5.242652667260116	29.20890375629478
>50	0.047642564805359486	1.2486472463053353
>100	0.020418012281933566	1.3548565433598654
>500	0.0014795661070701553	0.3600270009031801
>1k	0.0017754793284841864	1.2804906858984844
>5k	0.0	0.0
>10k+	2.9591322141403107E-4	1.8374898111022904
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16036	1.8316913675433335	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	2986	0.341071989491419	No Hit
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTG	2161	0.24683743110882664	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	1921	0.21942374139752707	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	1767	0.20183329049944318	No Hit
CGTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	1333	0.15226020160484308	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1007	0.11502327308032784	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1422370713041491E-4	0.0	0.0	0.08281218766955081	0.0
2	1.1422370713041491E-4	0.0	0.0	0.30200748165281704	0.0
3	1.1422370713041491E-4	0.0	0.0	0.7228076187212656	0.0
4	1.1422370713041491E-4	0.0	0.0	1.0818127302321596	0.0
5	1.1422370713041491E-4	0.0	0.0	1.943287929409749	0.0
6	1.1422370713041491E-4	0.0	0.0	3.3664010965475883	0.0
7	1.1422370713041491E-4	0.0	0.0	4.39635626374254	0.0
8	1.1422370713041491E-4	0.0	0.0	5.861732202518633	0.0
9	1.1422370713041491E-4	0.0	0.0	6.815500157057597	0.0
10	1.1422370713041491E-4	0.0	0.0	7.934321368400012	0.0
11	1.1422370713041491E-4	0.0	0.0	8.85907650132785	0.0
12	1.1422370713041491E-4	0.0	0.0	9.577886290299551	0.0
13	1.1422370713041491E-4	0.0	0.0	10.03192552614295	0.0
14	1.1422370713041491E-4	0.0	0.0	10.346840286701505	0.0
15	1.1422370713041491E-4	0.0	0.0	10.610811273879893	0.0
16	1.1422370713041491E-4	0.0	0.0	10.954396184928182	0.0
17	1.1422370713041491E-4	0.0	0.0	11.328593049487422	0.0
18	1.1422370713041491E-4	0.0	0.0	11.749621633970131	0.0
19	2.2844741426082983E-4	0.0	0.0	12.047631285873383	1.1422370713041491E-4
20	2.2844741426082983E-4	0.0	0.0	12.35763442702533	1.1422370713041491E-4
21	2.2844741426082983E-4	0.0	0.0	12.681572860447186	1.1422370713041491E-4
22	2.2844741426082983E-4	0.0	0.0	13.042291327565037	1.1422370713041491E-4
23	2.2844741426082983E-4	0.0	0.0	13.403124018390017	1.1422370713041491E-4
24	2.2844741426082983E-4	0.0	0.0	13.717467660412918	1.1422370713041491E-4
25	2.2844741426082983E-4	0.0	0.0	14.013992404123476	1.1422370713041491E-4
26	2.2844741426082983E-4	0.0	0.0	14.308232673691425	1.1422370713041491E-4
27	2.2844741426082983E-4	0.0	0.0	14.608184128615894	1.1422370713041491E-4
28	3.426711213912448E-4	0.0	0.0	14.927782061166795	1.1422370713041491E-4
29	3.426711213912448E-4	0.0	0.0	15.269082498072475	1.1422370713041491E-4
30	3.426711213912448E-4	0.0	0.0	15.714669179588224	1.1422370713041491E-4
31	3.426711213912448E-4	0.0	0.0	16.089779833804506	1.1422370713041491E-4
32	3.426711213912448E-4	0.0	0.0	16.488649019103914	1.1422370713041491E-4
33	3.426711213912448E-4	0.0	0.0	16.84262828750107	1.1422370713041491E-4
34	3.426711213912448E-4	0.0	0.0	17.183129158456836	1.1422370713041491E-4
35	3.426711213912448E-4	0.0	0.0	17.53893600616808	1.1422370713041491E-4
36	3.426711213912448E-4	0.0	0.0	17.891544590079672	1.1422370713041491E-4
37	3.426711213912448E-4	0.0	0.0	18.253862189097347	1.1422370713041491E-4
38	3.426711213912448E-4	0.0	0.0	18.666209771838144	1.1422370713041491E-4
39	3.426711213912448E-4	0.0	0.0	19.20500299837231	1.1422370713041491E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	40	6.8121153E-9	45.000004	2
CGGTCTA	45	3.8380676E-10	45.0	31
GCTAACG	25	3.890042E-5	45.0	1
CGTTTTT	9480	0.0	43.81329	1
CGTAAGG	110	0.0	42.954544	2
TCGACAC	60	3.6379788E-12	41.249996	34
TGATTCG	45	1.9274012E-8	40.0	15
GCGTAAG	70	0.0	38.571426	1
TGCGGGT	135	0.0	38.333332	4
CTAACGG	65	9.094947E-12	38.076927	2
GCGATAC	65	9.094947E-12	38.076927	9
TAACGGG	190	0.0	37.894737	3
GTTTTTT	11090	0.0	37.736702	2
CTACGAA	90	0.0	37.5	11
AACACGT	360	0.0	37.5	41
TATAGCG	30	1.13969974E-4	37.499996	1
GCGTACG	30	1.13969974E-4	37.499996	1
GCCGATT	55	2.748493E-9	36.81818	9
CGTTTCT	460	0.0	36.68478	1
AACCGGA	25	0.0021068351	36.0	29
>>END_MODULE