FastQCFastQC Report
Sat 18 Jun 2016
SRR3550326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621151
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC12520.2015612950796183TruSeq Adapter, Index 15 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC11970.1927067653436926TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11440.18417421850725507No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT8360.134588851986071TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG600.045.0000042
ACCGGTA207.0307805E-445.010
TCCGAAC253.888728E-545.043
CGCATGG700.045.02
CGTACGA207.0307805E-445.029
TTACGTA207.0307805E-445.016
ACCGTCC502.1827873E-1145.027
ACGACTA207.0307805E-445.039
CGAGACA1500.040.50000422
AACACGT1400.040.1785741
GTACACG451.925764E-840.01
GCACGAG451.925764E-840.01
GTTAGCG403.4548066E-739.3751
GCGTACG403.4548066E-739.3751
GTATGCG356.243654E-638.5714261
AATAGCG356.243654E-638.5714261
CGGGATC1000.038.256
TTGCGAG950.037.8947331
ACACGAC1550.037.74193626
GATCGAC301.1393179E-437.50000445