Basic Statistics
Measure | Value |
---|---|
Filename | SRR3550326_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 621151 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 1252 | 0.2015612950796183 | TruSeq Adapter, Index 15 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 1197 | 0.1927067653436926 | TruSeq Adapter, Index 15 (95% over 22bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1144 | 0.18417421850725507 | No Hit |
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT | 836 | 0.134588851986071 | TruSeq Adapter, Index 21 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAAAGG | 60 | 0.0 | 45.000004 | 2 |
ACCGGTA | 20 | 7.0307805E-4 | 45.0 | 10 |
TCCGAAC | 25 | 3.888728E-5 | 45.0 | 43 |
CGCATGG | 70 | 0.0 | 45.0 | 2 |
CGTACGA | 20 | 7.0307805E-4 | 45.0 | 29 |
TTACGTA | 20 | 7.0307805E-4 | 45.0 | 16 |
ACCGTCC | 50 | 2.1827873E-11 | 45.0 | 27 |
ACGACTA | 20 | 7.0307805E-4 | 45.0 | 39 |
CGAGACA | 150 | 0.0 | 40.500004 | 22 |
AACACGT | 140 | 0.0 | 40.17857 | 41 |
GTACACG | 45 | 1.925764E-8 | 40.0 | 1 |
GCACGAG | 45 | 1.925764E-8 | 40.0 | 1 |
GTTAGCG | 40 | 3.4548066E-7 | 39.375 | 1 |
GCGTACG | 40 | 3.4548066E-7 | 39.375 | 1 |
GTATGCG | 35 | 6.243654E-6 | 38.571426 | 1 |
AATAGCG | 35 | 6.243654E-6 | 38.571426 | 1 |
CGGGATC | 100 | 0.0 | 38.25 | 6 |
TTGCGAG | 95 | 0.0 | 37.894733 | 1 |
ACACGAC | 155 | 0.0 | 37.741936 | 26 |
GATCGAC | 30 | 1.1393179E-4 | 37.500004 | 45 |