FastQCFastQC Report
Sat 18 Jun 2016
SRR3550322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences777499
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.6454027593604622No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG12510.1609005284894257No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC10210.1313184968726648No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT8580.1103538396833951No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG253.8896367E-545.01
CTCCGAT207.0318766E-445.03
TCTAGCG207.0318766E-445.01
CGGTCTA351.2112469E-745.031
TCGGTGA253.8896367E-545.044
TACCGCT207.0318766E-445.015
TACGCGG650.041.5384642
CGTTTTT31200.040.3846171
GCGATAT356.245682E-638.5714269
TTGCGAG659.094947E-1238.0769271
TACGGGA2100.037.54
TCAAGCG3400.037.05882317
TAACGCG552.746674E-936.818181
CATACGG552.746674E-936.818182
ATTGCGG1100.036.818182
GCGAGAC3450.036.52173621
CGAGACA3450.036.52173622
CGGGTCA1050.036.428576
GGCCGAT1050.036.428578
AACACGT3100.036.2903241
Produced by FastQC (version 0.11.5)