##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550322_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	777499
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.354854475697074	33.0	31.0	34.0	31.0	34.0
2	32.48256525088778	34.0	31.0	34.0	31.0	34.0
3	32.53592480504798	34.0	31.0	34.0	31.0	34.0
4	36.037432845572795	37.0	35.0	37.0	35.0	37.0
5	36.0158225283891	37.0	35.0	37.0	35.0	37.0
6	36.027229617015585	37.0	35.0	37.0	35.0	37.0
7	36.18898545207132	37.0	36.0	37.0	35.0	37.0
8	36.18107032935091	37.0	37.0	37.0	35.0	37.0
9	37.77622993727323	39.0	38.0	39.0	35.0	39.0
10	37.508467535006474	39.0	37.0	39.0	35.0	39.0
11	37.457176150708875	39.0	37.0	39.0	35.0	39.0
12	37.351102702382896	39.0	37.0	39.0	35.0	39.0
13	37.313442203784184	39.0	37.0	39.0	34.0	39.0
14	38.54858334222938	40.0	38.0	41.0	34.0	41.0
15	38.60688695419544	40.0	38.0	41.0	35.0	41.0
16	38.62097057359559	40.0	38.0	41.0	35.0	41.0
17	38.62761752748235	40.0	38.0	41.0	35.0	41.0
18	38.538048280447946	40.0	38.0	41.0	35.0	41.0
19	38.43823721959771	40.0	37.0	41.0	34.0	41.0
20	38.30587434839145	40.0	37.0	41.0	34.0	41.0
21	38.25483505445023	40.0	37.0	41.0	34.0	41.0
22	38.22566974362668	40.0	37.0	41.0	34.0	41.0
23	38.18386776060162	40.0	37.0	41.0	34.0	41.0
24	38.16413397316266	40.0	37.0	41.0	34.0	41.0
25	38.11555899107266	40.0	36.0	41.0	34.0	41.0
26	37.99473697072279	40.0	36.0	41.0	34.0	41.0
27	37.94452983219271	40.0	36.0	41.0	34.0	41.0
28	37.84763195836908	40.0	36.0	41.0	33.0	41.0
29	37.76585050270161	40.0	36.0	41.0	33.0	41.0
30	37.71445879673157	40.0	36.0	41.0	33.0	41.0
31	37.586690143652916	40.0	36.0	41.0	33.0	41.0
32	37.36723262666576	40.0	35.0	41.0	33.0	41.0
33	37.17156549397491	40.0	35.0	41.0	32.0	41.0
34	36.94270603563477	40.0	35.0	41.0	31.0	41.0
35	36.86308921297648	40.0	35.0	41.0	31.0	41.0
36	36.71938613425869	39.0	35.0	41.0	31.0	41.0
37	36.669151986047574	39.0	35.0	41.0	31.0	41.0
38	36.539500372347746	39.0	35.0	41.0	30.0	41.0
39	36.465293202949454	39.0	35.0	41.0	30.0	41.0
40	36.39832591424555	39.0	35.0	41.0	30.0	41.0
41	36.26994246937938	39.0	35.0	41.0	30.0	41.0
42	36.18688641400182	39.0	35.0	41.0	30.0	41.0
43	36.11882973482924	39.0	35.0	41.0	30.0	41.0
44	35.955437884807566	39.0	35.0	41.0	29.0	41.0
45	35.86373101444504	38.0	35.0	41.0	29.0	41.0
46	35.767053076595595	38.0	35.0	40.0	29.0	41.0
47	35.68199830482097	38.0	35.0	40.0	28.0	41.0
48	35.56093319734173	38.0	35.0	40.0	28.0	41.0
49	35.45708740461402	38.0	35.0	40.0	27.0	41.0
50	35.36672072890126	38.0	35.0	40.0	27.0	41.0
51	34.13822654434282	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	8.0
11	3.0
12	6.0
13	8.0
14	13.0
15	28.0
16	42.0
17	101.0
18	154.0
19	293.0
20	559.0
21	943.0
22	1468.0
23	2233.0
24	3498.0
25	5678.0
26	7981.0
27	9353.0
28	9879.0
29	10632.0
30	12143.0
31	14499.0
32	18292.0
33	24785.0
34	44844.0
35	58314.0
36	57042.0
37	83809.0
38	145410.0
39	265294.0
40	177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.2664209214417	19.96336972780672	23.799387523328004	13.97082182742357
2	36.077602672157774	23.540737672974497	23.028582673418228	17.353076981449494
3	31.5824200416978	24.096751249840835	27.423443631438754	16.897385077022605
4	28.192190600888235	25.479904154217564	27.510774933472582	18.81713031142162
5	25.685177730132132	29.736372651283155	25.519775588135808	19.058674030448913
6	26.679133992455295	34.15052623861896	24.649935241074264	14.520404527851483
7	80.53901033956313	5.777370774753408	9.462777444086745	4.2208414415967095
8	81.17926839777286	4.917433977407045	9.77570389158057	4.127593733239529
9	74.77141449699613	7.111005930554251	11.98805400392798	6.129525568521632
10	38.73111090818123	25.93379541324169	19.459832102677947	15.875261575899133
11	30.6368239701916	25.805306502001933	25.491736966864266	18.0661325609422
12	27.720421505365277	23.407104060584	28.952448813438984	19.92002562061173
13	24.569677903122706	23.56517500344052	31.229622160285736	20.63552493315104
14	21.353210743679412	26.368651278008077	30.91978253348236	21.358355444830153
15	21.501506754349524	25.532765958541425	33.218949477748524	19.746777809360527
16	24.30575473408969	24.65829538044422	31.538432846858967	19.497517038607125
17	24.263310949596075	23.835400431383192	29.995151119165424	21.906137499855305
18	24.671800220964915	24.532764672366138	29.38408923998616	21.411345866682787
19	24.507041166612435	26.571095268289735	28.099457362646124	20.822406202451706
20	27.028330583061845	25.294823530319654	27.68286518696487	19.993980699653633
21	25.257910299563086	25.98357039687511	28.07733514769794	20.681184155863868
22	23.753085212971335	24.50446881603706	28.245695492855937	23.496750478135663
23	23.451991578124215	26.67900537492653	28.212512170433662	21.656490876515598
24	23.113598859934225	24.768777837656383	29.802353443541406	22.315269858867985
25	23.591670214366836	26.174181574510065	27.904087336446736	22.330060874676366
26	22.47308356666697	26.94125651608555	28.671290895550992	21.91436902169649
27	23.85057729977788	25.582412324646075	28.686081911359373	21.880928464216673
28	22.227295469190313	26.845822309739305	28.777143121727487	22.149739099342895
29	23.260222842730343	25.8814480790329	28.77418491856581	22.084144159670945
30	24.382153546178195	24.686462619244526	28.878107881810784	22.053275952766498
31	25.236173937201205	25.830258302583026	26.875532958884836	22.058034801330933
32	24.88247573308776	25.342669251021544	28.621901764503875	21.15295325138682
33	25.12697765527673	25.35192971309288	27.15579055407145	22.365302077558944
34	24.49507973643696	25.66048316460857	28.759265285228665	21.085171813725808
35	25.016237963007026	24.710128244537934	28.208267791984298	22.06536600047074
36	24.73675207299302	27.121835526476563	26.55514669472244	21.586265705807982
37	25.32144735877474	26.205435634000818	27.651868362531655	20.82124864469279
38	24.163117894685396	26.321577262478797	27.226401577365372	22.288903265470438
39	24.94536970465557	24.50884181201519	27.869875073794308	22.67591340953493
40	24.760546315815198	24.310642200182894	29.183188660049726	21.745622823952186
41	22.133147438131754	25.710772618357066	28.585631621391155	23.570448322120026
42	23.367875714309598	25.542926743314137	28.480165247800958	22.6090322945753
43	23.599258648564177	24.827170195717294	28.919908578660554	22.653662577057975
44	24.107940974843697	25.37109372487939	27.83553419361311	22.685431106663803
45	23.70189543652146	24.199902507913194	27.871932954254603	24.22626910131074
46	23.508197438196063	25.38138312718087	27.353475695788674	23.75694373883439
47	23.16195905075119	25.415338154775764	29.350520064977577	22.07218272949547
48	23.29482095796908	23.995657872228772	29.292256324445432	23.417264845356716
49	23.382923965175518	24.323503953059745	29.05341357352228	23.24015850824245
50	21.935333678885762	24.45829512320916	29.436179339137414	24.170191858767666
51	22.725559775639585	23.8243393239091	27.8948268743754	25.55527402607592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	310.0
1	675.0
2	1040.0
3	4915.5
4	8791.0
5	5919.0
6	3047.0
7	3028.0
8	3009.0
9	2961.5
10	2914.0
11	2970.0
12	3026.0
13	2945.5
14	2865.0
15	2824.0
16	2783.0
17	2773.5
18	2764.0
19	2811.0
20	2858.0
21	2903.0
22	2948.0
23	3233.5
24	3519.0
25	3818.0
26	5126.0
27	6135.0
28	6944.5
29	7754.0
30	9397.5
31	11041.0
32	12816.0
33	14591.0
34	16195.5
35	17800.0
36	20032.0
37	22264.0
38	24615.5
39	26967.0
40	29966.5
41	32966.0
42	35269.5
43	37573.0
44	39706.5
45	41840.0
46	45622.0
47	49404.0
48	54133.0
49	58862.0
50	63437.0
51	68012.0
52	67951.5
53	67891.0
54	61379.0
55	54867.0
56	48779.0
57	42691.0
58	41195.0
59	39699.0
60	37545.0
61	35391.0
62	33218.0
63	31045.0
64	26753.0
65	22461.0
66	18232.5
67	14004.0
68	12034.0
69	10064.0
70	8544.5
71	7025.0
72	6020.5
73	5016.0
74	4348.0
75	2977.5
76	2275.0
77	1687.5
78	1100.0
79	857.5
80	615.0
81	455.0
82	295.0
83	191.5
84	88.0
85	64.0
86	40.0
87	32.5
88	25.0
89	18.5
90	12.0
91	10.5
92	9.0
93	6.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	777499.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.07900310142457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.60681159861518	24.01760946055141
2	8.418164260290785	5.569289633489206
3	3.2474577421449204	3.222679941724712
4	1.9159571414726628	2.5351180889988307
5	1.383511477454995	2.288259022679513
6	1.1792928974383505	2.34059000471107
7	1.0240868091456479	2.3713039515099816
8	0.9097161449914228	2.407400254526982
9	0.8713908568315002	2.594226677011398
>10	8.350217925318843	48.32972259323277
>50	0.06291337536943976	1.4018420281802069
>100	0.027353640574847772	1.5062853605597428
>500	0.0019538314696319757	0.47335595901225114
>1k	7.815325878527901E-4	0.2936823426749958
>5k	3.9076629392639507E-4	0.6486346811369406
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5018	0.6454027593604622	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	1251	0.1609005284894257	No Hit
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1021	0.1313184968726648	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTT	858	0.1103538396833951	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.572350575370515E-4	0.0	0.0	0.10327987560112617	0.0
2	2.572350575370515E-4	0.0	0.0	0.4110616219442083	0.0
3	2.572350575370515E-4	0.0	0.0	0.7304189458764577	0.0
4	2.572350575370515E-4	0.0	0.0	1.0043742821534176	0.0
5	2.572350575370515E-4	0.0	0.0	1.751384889241015	0.0
6	2.572350575370515E-4	0.0	0.0	2.534022551797494	0.0
7	2.572350575370515E-4	0.0	0.0	3.2055346694979674	0.0
8	2.572350575370515E-4	0.0	0.0	4.234474899646173	0.0
9	2.572350575370515E-4	0.0	0.0	4.766565616161564	0.0
10	2.572350575370515E-4	0.0	0.0	5.647981540812271	0.0
11	2.572350575370515E-4	0.0	0.0	6.632805958592873	0.0
12	2.572350575370515E-4	0.0	0.0	7.385218501888748	0.0
13	2.572350575370515E-4	0.0	0.0	7.773257586183391	0.0
14	2.572350575370515E-4	0.0	0.0	7.975572958936281	0.0
15	2.572350575370515E-4	0.0	0.0	8.243354653832352	0.0
16	2.572350575370515E-4	0.0	0.0	8.728499972347231	0.0
17	2.572350575370515E-4	0.0	0.0	9.265478154955826	0.0
18	2.572350575370515E-4	0.0	0.0	9.83911233326345	0.0
19	2.572350575370515E-4	0.0	0.0	10.204643350023602	0.0
20	3.858525863055772E-4	0.0	0.0	10.566058605863159	0.0
21	3.858525863055772E-4	0.0	0.0	11.010303550229647	0.0
22	3.858525863055772E-4	0.0	0.0	11.491976195467776	0.0
23	3.858525863055772E-4	0.0	0.0	11.974420545878516	0.0
24	3.858525863055772E-4	0.0	0.0	12.37184870977326	0.0
25	3.858525863055772E-4	0.0	0.0	12.755000327974699	0.0
26	3.858525863055772E-4	0.0	0.0	13.098151894729124	0.0
27	3.858525863055772E-4	0.0	0.0	13.442461019242469	0.0
28	3.858525863055772E-4	0.0	0.0	13.817638350660259	0.0
29	3.858525863055772E-4	0.0	0.0	14.1987320884014	0.0
30	3.858525863055772E-4	0.0	0.0	14.65686772587489	0.0
31	3.858525863055772E-4	0.0	0.0	15.063170499254662	0.0
32	3.858525863055772E-4	0.0	0.0	15.50034147953888	0.0
33	3.858525863055772E-4	0.0	0.0	15.89648346814594	0.0
34	5.14470115074103E-4	0.0	0.0	16.296612600144822	0.0
35	5.14470115074103E-4	0.0	0.0	16.692240118636807	0.0
36	5.14470115074103E-4	0.0	0.0	17.094684366153526	0.0
37	5.14470115074103E-4	0.0	0.0	17.52979746597745	0.0
38	6.430876438426287E-4	0.0	0.0	17.948061669532695	0.0
39	6.430876438426287E-4	0.0	0.0	18.371084721652377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	25	3.8896367E-5	45.0	1
CTCCGAT	20	7.0318766E-4	45.0	3
TCTAGCG	20	7.0318766E-4	45.0	1
CGGTCTA	35	1.2112469E-7	45.0	31
TCGGTGA	25	3.8896367E-5	45.0	44
TACCGCT	20	7.0318766E-4	45.0	15
TACGCGG	65	0.0	41.538464	2
CGTTTTT	3120	0.0	40.384617	1
GCGATAT	35	6.245682E-6	38.571426	9
TTGCGAG	65	9.094947E-12	38.076927	1
TACGGGA	210	0.0	37.5	4
TCAAGCG	340	0.0	37.058823	17
TAACGCG	55	2.746674E-9	36.81818	1
CATACGG	55	2.746674E-9	36.81818	2
ATTGCGG	110	0.0	36.81818	2
GCGAGAC	345	0.0	36.521736	21
CGAGACA	345	0.0	36.521736	22
CGGGTCA	105	0.0	36.42857	6
GGCCGAT	105	0.0	36.42857	8
AACACGT	310	0.0	36.29032	41
>>END_MODULE