SRR3550321_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	608146
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2104	0.3459695533638304	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	1548	0.2545441390718676	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	1364	0.22428824657236912	TruSeq Adapter, Index 8 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1135	0.18663281514636287	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1033	0.16986052691294523	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	696	0.11444620206332032	TruSeq Adapter, Index 13 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	55	1.8189894E-12	45.000004	2
CTATGCG	25	3.888631E-5	45.0	1
CCAATCG	20	7.0306635E-4	45.0	24
TCTAGCG	25	3.888631E-5	45.0	1
TCGGCTA	20	7.0306635E-4	45.0	33
ATCGTAG	20	7.0306635E-4	45.0	1
GTAATCG	35	1.2106648E-7	45.0	1
TCGGACG	20	7.0306635E-4	45.0	1
TTACGGT	20	7.0306635E-4	45.0	34
TTCGCTT	20	7.0306635E-4	45.0	3
AATCCGG	30	2.1638098E-6	44.999996	2
ATCTCGG	30	2.1638098E-6	44.999996	2
TAACGCC	30	2.1638098E-6	44.999996	12
GCGATTC	30	2.1638098E-6	44.999996	9
CGAATAT	170	0.0	43.676468	14
GCGATAT	70	0.0	41.785713	9
GTTCGAT	50	1.0804797E-9	40.5	39
GCTACGA	190	0.0	40.263157	10
CGGGTAT	45	1.925764E-8	40.000004	6
ATGCGAA	45	1.925764E-8	40.000004	26