FastQCFastQC Report
Sat 18 Jun 2016
SRR3550318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences950405
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136291.4340202334794114No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT41330.43486724080786615No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG33890.35658482436434996No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC28930.3043965467353391No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT24600.2588370221116261No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT17200.18097547887479548No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC13230.13920381311125257No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT11190.11773927957028846Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC10390.10932181543657704No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.032669E-445.00000421
TATAGCG406.8121153E-945.0000041
TTACGAG406.8121153E-945.0000041
CGTTTTT75550.043.093981
CGGTCTA451.927765E-840.00000431
CGTTTCT5950.038.949581
CGCCCTA356.247148E-638.57142625
ACACGAC5750.038.34782826
ACGACCA5700.037.89473328
CGTAAGG1250.037.82
CTATCGG301.1397731E-437.5000042
GCTATCG301.1397731E-437.5000041
TACGACG301.1397731E-437.5000041
GCGATTA900.037.5000049
AGACACG5900.037.37288324
ATAGGGA5600.037.366074
CACGTGA5250.037.28571743
ACAACGA3500.037.28571313
CGTTCTG3700.037.0945931
GTTTTTT89650.037.0691572