##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950405
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26154323683061	33.0	31.0	34.0	30.0	34.0
2	32.41025562786391	34.0	31.0	34.0	30.0	34.0
3	32.411054234773594	34.0	31.0	34.0	30.0	34.0
4	35.97301887090241	37.0	35.0	37.0	35.0	37.0
5	35.946595398803666	37.0	35.0	37.0	35.0	37.0
6	35.96954456258121	37.0	35.0	37.0	35.0	37.0
7	36.130646408636316	37.0	35.0	37.0	35.0	37.0
8	36.115810628100654	37.0	35.0	37.0	35.0	37.0
9	37.71880198441717	39.0	38.0	39.0	35.0	39.0
10	37.48788358647103	39.0	37.0	39.0	35.0	39.0
11	37.42815957407632	39.0	37.0	39.0	35.0	39.0
12	37.32700269884944	39.0	37.0	39.0	35.0	39.0
13	37.28557299256633	39.0	37.0	39.0	34.0	39.0
14	38.52484888021422	40.0	38.0	41.0	34.0	41.0
15	38.57917308936717	40.0	38.0	41.0	34.0	41.0
16	38.57797360072811	40.0	38.0	41.0	34.0	41.0
17	38.55777379117324	40.0	38.0	41.0	34.0	41.0
18	38.406501438860275	40.0	38.0	41.0	34.0	41.0
19	38.248930719009266	40.0	37.0	41.0	34.0	41.0
20	38.06012173757503	40.0	36.0	41.0	34.0	41.0
21	37.99026309836333	40.0	36.0	41.0	34.0	41.0
22	37.96281795655536	40.0	35.0	41.0	34.0	41.0
23	37.91344005976399	40.0	35.0	41.0	34.0	41.0
24	37.870547819087655	40.0	35.0	41.0	34.0	41.0
25	37.81133201109001	40.0	35.0	41.0	34.0	41.0
26	37.6879761785765	40.0	35.0	41.0	33.0	41.0
27	37.62277134484772	40.0	35.0	41.0	33.0	41.0
28	37.501961795234664	40.0	35.0	41.0	33.0	41.0
29	37.41409820024095	39.0	35.0	41.0	33.0	41.0
30	37.311151561702644	39.0	35.0	41.0	33.0	41.0
31	37.13317690879151	39.0	35.0	41.0	32.0	41.0
32	36.829949337387745	39.0	35.0	41.0	31.0	41.0
33	36.53288334972985	39.0	35.0	41.0	30.0	41.0
34	36.17048205764911	39.0	35.0	41.0	29.0	41.0
35	36.021227792362204	39.0	35.0	41.0	29.0	41.0
36	35.82961474318843	39.0	35.0	41.0	27.0	41.0
37	35.749516258858065	39.0	35.0	41.0	27.0	41.0
38	35.56814936790105	39.0	35.0	41.0	26.0	41.0
39	35.45335093986248	39.0	35.0	41.0	25.0	41.0
40	35.33997611544552	38.0	35.0	41.0	25.0	41.0
41	35.19961910974795	38.0	35.0	40.0	24.0	41.0
42	35.094471304338676	38.0	35.0	40.0	24.0	41.0
43	35.0081901926021	38.0	35.0	40.0	23.0	41.0
44	34.822348367275005	38.0	34.0	40.0	23.0	41.0
45	34.75442995354612	38.0	34.0	40.0	23.0	41.0
46	34.65429474802847	38.0	34.0	40.0	23.0	41.0
47	34.55399961069229	37.0	34.0	40.0	23.0	41.0
48	34.42605520804289	37.0	34.0	40.0	23.0	41.0
49	34.329517416259385	37.0	34.0	40.0	22.0	41.0
50	34.23310378207185	37.0	34.0	40.0	22.0	41.0
51	33.02385509335494	35.0	32.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	7.0
12	6.0
13	16.0
14	21.0
15	31.0
16	65.0
17	119.0
18	280.0
19	535.0
20	1007.0
21	1537.0
22	2455.0
23	3841.0
24	6154.0
25	10095.0
26	14999.0
27	17583.0
28	17578.0
29	17371.0
30	18099.0
31	20704.0
32	24922.0
33	32435.0
34	56058.0
35	72173.0
36	72943.0
37	102140.0
38	172883.0
39	284119.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.11037925936837	19.420247157790627	22.06164740294927	19.40772617989173
2	37.895107875063786	22.54060111215745	22.394137236230872	17.17015377654789
3	29.41861627411472	22.935906271536872	31.146616442464005	16.498861011884408
4	26.729026046790576	24.136657530210805	30.830119791036452	18.304196631962164
5	24.210625996285795	28.049726169369897	28.802773554432058	18.936874279912246
6	25.163167281316912	32.39955597876695	28.118328502059647	14.318948237856494
7	75.58346178734328	5.447361914131344	14.093044544168013	4.8761317543573535
8	76.20372367569615	4.893071900926447	14.184479248320454	4.718725175056949
9	69.63862774290959	6.73670698281259	16.70077493279181	6.923890341485999
10	37.218764631920074	24.53659229486377	22.764821313019187	15.479821760196968
11	28.920197179097336	23.41570172715842	28.966914105039432	18.697186988704814
12	25.43484093623245	21.69222594578101	32.22342054176904	20.649512576217507
13	23.04101935490659	21.686649375792424	34.610929024994604	20.661402244306373
14	19.589859060084912	24.471251729525832	34.4697260641516	21.46916314623766
15	20.298083448635055	23.432431437124173	35.554842409288675	20.714642704952098
16	23.090682393295488	23.222626143591416	33.89386629910406	19.792825164009027
17	21.75798738432563	23.422540916767062	32.822743988089286	21.99672771081802
18	23.046280269990163	23.276287477443827	31.89755946149273	21.77987279107328
19	22.423177487492175	26.10455542637086	30.012468368748056	21.459798717388903
20	25.029960911400927	24.479774411961216	29.581073331895354	20.909191344742503
21	23.362145611607684	26.124231248783413	30.041613838311036	20.472009301297867
22	23.069954387866225	22.94495504548061	30.29045512176388	23.694635444889283
23	22.498513791488893	25.11550339065977	30.281932439328497	22.104050378522842
24	22.263140450649985	23.047437671308547	32.27750274882813	22.411919129213334
25	23.44421588691137	24.46546472293391	29.651780030618525	22.438539359536197
26	21.899611218375327	24.838463602358996	30.606951773191426	22.65497340607425
27	22.75230033512029	23.199688553827052	30.162404448629793	23.885606662422862
28	21.117207927146847	25.48881792498987	29.944497345868342	23.44947680199494
29	22.123094891125362	23.584682319642678	30.006786580457806	24.285436208774154
30	22.635087147058357	22.638454132711843	30.950910401355213	23.775548318874584
31	22.611413029182295	24.164540380153724	28.496062205059946	24.72798438560403
32	22.473892708897786	23.498508530573808	29.487744698312824	24.539854062215582
33	22.295442469263104	24.305112031186702	27.506799732745513	25.892645766804677
34	21.166029219122372	25.305527643478303	29.75342091003309	23.77502222736623
35	22.036395010548134	24.226303523234833	29.398203923590472	24.339097542626565
36	22.785233663543437	27.099499686975552	26.772270768777524	23.34299588070349
37	24.208311193649024	25.290481426339294	27.274898595861764	23.226308784149914
38	23.666016066834665	25.768593389134104	27.608861485366766	22.956529058664465
39	24.33878188772155	24.069107380537773	27.579295142597104	24.012815589143578
40	24.85466722081639	23.449055928788255	30.10969007949243	21.58658677090293
41	22.81122258405627	24.755341144038596	28.984801216323568	23.44863505558157
42	23.686217980755572	24.914115561260726	28.253007928199033	23.14665852978467
43	23.401707693036126	23.46105081517879	29.046353922801334	24.09088756898375
44	23.537860175398908	24.164540380153724	27.739753052645977	24.55784639180139
45	22.869303086578878	22.805646014067687	28.582025557525476	25.74302534182796
46	22.711265197468446	24.109511208379587	27.88379690763411	25.295426686517853
47	22.525449676716768	23.96041687491122	29.744372136089353	23.769761312282657
48	22.429911458799143	23.149920297136482	29.837595551370207	24.582572692694168
49	22.649291617783998	23.215997390586118	29.17840289139893	24.956308100230952
50	20.74862821639196	22.86014909433347	30.984369821286716	25.406852867987855
51	21.526401902346894	22.657182990409353	29.410724901489367	26.40569020575439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	464.0
1	949.5
2	1435.0
3	11586.0
4	21737.0
5	14389.5
6	7042.0
7	6830.5
8	6619.0
9	6547.5
10	6476.0
11	6520.0
12	6564.0
13	6303.0
14	6042.0
15	5783.5
16	5525.0
17	5214.0
18	4903.0
19	4612.0
20	4321.0
21	4391.0
22	4461.0
23	4513.0
24	4565.0
25	4680.0
26	5559.5
27	6324.0
28	7236.0
29	8148.0
30	9798.0
31	11448.0
32	12732.5
33	14017.0
34	15921.5
35	17826.0
36	20290.5
37	22755.0
38	24936.5
39	27118.0
40	29383.5
41	31649.0
42	34639.0
43	37629.0
44	40813.5
45	43998.0
46	48829.0
47	53660.0
48	64275.5
49	74891.0
50	80379.0
51	85867.0
52	86160.5
53	86454.0
54	77125.0
55	67796.0
56	60408.5
57	53021.0
58	50096.0
59	47171.0
60	44891.5
61	42612.0
62	39438.0
63	36264.0
64	32394.5
65	28525.0
66	23927.0
67	19329.0
68	16976.5
69	14624.0
70	12796.5
71	10969.0
72	9633.0
73	8297.0
74	6986.0
75	5067.5
76	4460.0
77	3476.0
78	2492.0
79	1889.0
80	1286.0
81	941.5
82	597.0
83	460.5
84	324.0
85	230.5
86	137.0
87	90.0
88	43.0
89	32.5
90	22.0
91	22.0
92	22.0
93	13.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	950405.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.52610981688433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01157829299434	25.938173483409656
2	8.164561021451142	5.801101829094532
3	3.332354233306112	3.551567473235769
4	2.1397572815215193	3.040690086592455
5	1.6666539879125242	2.960486630066427
6	1.4118694289120883	3.009493702717957
7	1.2211851313099014	3.03687699571633
8	1.065292243904964	3.02765513952343
9	0.9884998210819144	3.1605797877953945
>10	6.899091198673412	39.38531711167236
>50	0.06759393978249884	1.617152985513949
>100	0.02709689757275212	1.6735254083401911
>500	0.0017866086307722232	0.44345249752403004
>1k	0.002382144841029631	1.9121773247243241
>5k	0.0	0.0
>10k+	2.977681051287039E-4	1.4417495440732362
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13629	1.4340202334794114	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	4133	0.43486724080786615	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	3389	0.35658482436434996	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	2893	0.3043965467353391	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	2460	0.2588370221116261	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	1720	0.18097547887479548	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	1323	0.13920381311125257	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	1119	0.11773927957028846	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC	1039	0.10932181543657704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.260915083569636E-4	0.0	0.0	0.13667857387113916	0.0
2	5.260915083569636E-4	0.0	0.0	0.4548587181254307	0.0
3	5.260915083569636E-4	0.0	0.0	1.0334541590164192	0.0
4	5.260915083569636E-4	0.0	0.0	1.4882076588401787	0.0
5	5.260915083569636E-4	0.0	0.0	2.582477996222663	0.0
6	5.260915083569636E-4	0.0	0.0	4.053114198683719	0.0
7	6.313098100283564E-4	0.0	0.0	5.145806261541132	0.0
8	6.313098100283564E-4	0.0	0.0	6.591084853299383	0.0
9	6.313098100283564E-4	0.0	0.0	7.466185468300356	0.0
10	6.313098100283564E-4	0.0	0.0	8.638212130618	0.0
11	7.36528111699749E-4	0.0	0.0	9.71322751879462	0.0
12	7.36528111699749E-4	0.0	0.0	10.496893429643151	0.0
13	7.36528111699749E-4	0.0	0.0	10.94017813458473	0.0
14	7.36528111699749E-4	0.0	0.0	11.195122079534514	0.0
15	7.36528111699749E-4	0.0	0.0	11.473740142360363	0.0
16	7.36528111699749E-4	0.0	0.0	11.90639779883313	0.0
17	7.36528111699749E-4	0.0	0.0	12.38692978256638	0.0
18	7.36528111699749E-4	0.0	0.0	12.920702226945355	0.0
19	7.36528111699749E-4	0.0	0.0	13.26560781982418	0.0
20	7.36528111699749E-4	0.0	0.0	13.60178029366428	0.0
21	7.36528111699749E-4	0.0	0.0	14.019286514696367	0.0
22	7.36528111699749E-4	0.0	0.0	14.4689895360399	0.0
23	7.36528111699749E-4	0.0	0.0	14.931634408489012	0.0
24	7.36528111699749E-4	0.0	0.0	15.307895055265913	0.0
25	7.36528111699749E-4	0.0	0.0	15.663006823406864	0.0
26	7.36528111699749E-4	0.0	0.0	16.021169922296284	0.0
27	8.417464133711418E-4	0.0	0.0	16.358289360851426	0.0
28	8.417464133711418E-4	0.0	0.0	16.698775785060054	0.0
29	8.417464133711418E-4	0.0	0.0	17.045470089067294	0.0
30	8.417464133711418E-4	0.0	0.0	17.490753941740625	0.0
31	8.417464133711418E-4	0.0	0.0	17.864068476070727	0.0
32	8.417464133711418E-4	0.0	0.0	18.272525923159073	0.0
33	8.417464133711418E-4	0.0	0.0	18.644262182964106	0.0
34	8.417464133711418E-4	0.0	0.0	18.992429543194742	0.0
35	8.417464133711418E-4	0.0	0.0	19.37163630241844	0.0
36	8.417464133711418E-4	0.0	0.0	19.75526223031234	0.0
37	8.417464133711418E-4	0.0	0.0	20.150567389691762	0.0
38	8.417464133711418E-4	0.0	0.0	20.574281490522463	0.0
39	8.417464133711418E-4	0.0	0.0	20.98294937421415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.032669E-4	45.000004	21
TATAGCG	40	6.8121153E-9	45.000004	1
TTACGAG	40	6.8121153E-9	45.000004	1
CGTTTTT	7555	0.0	43.09398	1
CGGTCTA	45	1.927765E-8	40.000004	31
CGTTTCT	595	0.0	38.94958	1
CGCCCTA	35	6.247148E-6	38.571426	25
ACACGAC	575	0.0	38.347828	26
ACGACCA	570	0.0	37.894733	28
CGTAAGG	125	0.0	37.8	2
CTATCGG	30	1.1397731E-4	37.500004	2
GCTATCG	30	1.1397731E-4	37.500004	1
TACGACG	30	1.1397731E-4	37.500004	1
GCGATTA	90	0.0	37.500004	9
AGACACG	590	0.0	37.372883	24
ATAGGGA	560	0.0	37.36607	4
CACGTGA	525	0.0	37.285717	43
ACAACGA	350	0.0	37.285713	13
CGTTCTG	370	0.0	37.094593	1
GTTTTTT	8965	0.0	37.069157	2
>>END_MODULE