FastQCFastQC Report
Sat 18 Jun 2016
SRR3550317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836234
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178352.132776232489949No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10520.12580210802239564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG302.1647884E-645.0000041
GTAGACG253.8898906E-545.01
AAATTCG253.8898906E-545.030
CGTACAT253.8898906E-545.035
ACGAACG207.032184E-445.021
CGAATAT1800.041.2514
TACGAAT1750.041.14285712
AAACACG5650.041.01769640
ATGGGAC3000.040.55
GCGTAAG1000.040.51
TAGCCGT950.040.26315744
CTACGAA1800.040.011
CCTATGC1800.040.035
ACGACCA5950.039.70588328
AACACGT5800.039.5689741
GACGAAT403.4567165E-739.37544
CACGTGA5900.039.27966343
CGTAAGG1550.039.1935462
CGTTTTT36900.039.024391
TCAAGCG6000.039.017
Produced by FastQC (version 0.11.5)