SRR3550315_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279841
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	876	0.3130349019621857	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	649	0.23191741024367407	TruSeq Adapter, Index 27 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	585	0.20904728041995277	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	553	0.1976122155080921	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	476	0.17009659056392737	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	424	0.1515146100821538	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	399	0.14258096561976266	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	280	0.1000568179787808	TruSeq Adapter, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	7.0241286E-4	45.000004	34
AATCACG	20	7.0241286E-4	45.000004	1
CATGATA	20	7.0241286E-4	45.000004	40
CCGGCAA	20	7.0241286E-4	45.000004	34
GACGTAG	50	2.1827873E-11	45.000004	1
GAACTCG	30	2.1595952E-6	45.000004	9
CGTTGCC	20	7.0241286E-4	45.000004	22
CCAATGC	25	3.8832164E-5	45.000004	34
GCGTTGT	30	2.1595952E-6	45.000004	44
GCGTTAG	35	1.2075179E-7	45.000004	1
GGCCCAT	25	3.8832164E-5	45.000004	8
GTAATAG	45	3.8198777E-10	45.000004	1
CGTTAGG	45	3.8198777E-10	45.000004	2
TTGAGCG	25	3.8832164E-5	45.000004	1
CACCTAA	20	7.0241286E-4	45.000004	28
AGCATCG	20	7.0241286E-4	45.000004	24
CTACGCG	35	1.2075179E-7	45.000004	1
AACGATG	25	3.8832164E-5	45.000004	21
GTGTAAG	40	6.7830115E-9	45.000004	1
CGGGATC	20	7.0241286E-4	45.000004	6