##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279841
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.317605354469144	33.0	31.0	34.0	30.0	34.0
2	32.42313670977448	34.0	31.0	34.0	31.0	34.0
3	32.425312945565516	34.0	31.0	34.0	30.0	34.0
4	35.97540031660836	37.0	35.0	37.0	35.0	37.0
5	36.00447754260455	37.0	35.0	37.0	35.0	37.0
6	35.991548772338575	37.0	35.0	37.0	35.0	37.0
7	36.186062799947116	37.0	36.0	37.0	35.0	37.0
8	36.11395042184669	37.0	37.0	37.0	35.0	37.0
9	37.93675694412184	39.0	38.0	39.0	35.0	39.0
10	37.60881357628082	39.0	37.0	39.0	35.0	39.0
11	37.43770212370596	39.0	37.0	39.0	35.0	39.0
12	37.2435990437427	39.0	37.0	39.0	35.0	39.0
13	37.148034062199606	39.0	37.0	39.0	33.0	39.0
14	38.369066720030304	40.0	38.0	41.0	34.0	41.0
15	38.52472296768522	40.0	38.0	41.0	35.0	41.0
16	38.5344427728603	40.0	37.0	41.0	35.0	41.0
17	38.516714848789135	40.0	37.0	41.0	35.0	41.0
18	38.49187217026812	40.0	37.0	41.0	35.0	41.0
19	38.36357788887261	40.0	37.0	41.0	34.0	41.0
20	38.31887393198281	40.0	37.0	41.0	34.0	41.0
21	38.16355358935967	40.0	36.0	41.0	34.0	41.0
22	38.26722674661683	40.0	37.0	41.0	34.0	41.0
23	38.22788654986224	40.0	36.0	41.0	34.0	41.0
24	38.16980356702556	40.0	36.0	41.0	34.0	41.0
25	37.80788018910739	40.0	36.0	41.0	34.0	41.0
26	37.74259311537623	40.0	35.0	41.0	34.0	41.0
27	37.86685296293252	40.0	36.0	41.0	34.0	41.0
28	37.94720930814284	40.0	36.0	41.0	34.0	41.0
29	37.994861367705234	40.0	36.0	41.0	34.0	41.0
30	37.83863336680472	40.0	36.0	41.0	34.0	41.0
31	37.893718218559826	40.0	36.0	41.0	34.0	41.0
32	37.82212756529601	40.0	35.0	41.0	34.0	41.0
33	37.759695684335036	40.0	35.0	41.0	34.0	41.0
34	37.715034608938645	40.0	35.0	41.0	34.0	41.0
35	37.68006475105506	40.0	35.0	41.0	33.0	41.0
36	37.582162728120615	40.0	35.0	41.0	33.0	41.0
37	37.53722649647479	40.0	35.0	41.0	33.0	41.0
38	37.487162352907546	40.0	35.0	41.0	33.0	41.0
39	37.388131117313044	40.0	35.0	41.0	33.0	41.0
40	37.281388359818614	40.0	35.0	41.0	33.0	41.0
41	37.25061016791678	40.0	35.0	41.0	33.0	41.0
42	37.24964533431484	39.0	35.0	41.0	33.0	41.0
43	37.207253404611905	39.0	35.0	41.0	33.0	41.0
44	37.07646127622471	39.0	35.0	41.0	33.0	41.0
45	36.97445692375313	39.0	35.0	41.0	32.0	41.0
46	36.900743636565046	39.0	35.0	41.0	32.0	41.0
47	36.83137924750126	39.0	35.0	41.0	32.0	41.0
48	36.79235351503175	39.0	35.0	41.0	32.0	41.0
49	36.66150778477778	39.0	35.0	41.0	32.0	41.0
50	36.58373504954599	39.0	35.0	41.0	32.0	41.0
51	35.70961010002108	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	4.0
11	7.0
12	3.0
13	5.0
14	2.0
15	8.0
16	15.0
17	29.0
18	49.0
19	90.0
20	179.0
21	239.0
22	420.0
23	568.0
24	857.0
25	1123.0
26	1517.0
27	1788.0
28	2121.0
29	2685.0
30	3273.0
31	4202.0
32	5663.0
33	8155.0
34	17889.0
35	26876.0
36	17836.0
37	26464.0
38	50983.0
39	106702.0
40	81.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.46089743818812	19.691539124002556	25.419791953287763	12.427771484521568
2	29.1011681633499	25.430512326642628	26.953520034591072	18.514799475416396
3	31.76518094203494	23.89178140444038	26.67621971047845	17.66681794304623
4	28.859602417086844	25.623836392808773	25.69208943650144	19.82447175360294
5	25.119264153572924	30.40119210551706	24.6286283996984	19.850915341211618
6	27.201875350645544	34.01931811278548	23.76135019528947	15.017456341279512
7	82.08125328311434	5.811157049896191	7.505333385744048	4.602256281245422
8	83.85833383957319	4.983901572678771	6.696659889008401	4.461104698739642
9	78.65144850111314	6.391486594173119	8.50876033175982	6.448304572953928
10	49.71287266697875	20.191823213896463	15.251875171972657	14.843428947152132
11	34.717571763965964	20.850054137885444	21.70375320271154	22.72862089543705
12	27.252975796970425	19.890223376846137	28.53942059955475	24.317380226628693
13	20.77465417862286	24.523926086599175	30.664198598489854	24.037221136288107
14	16.866006053437488	26.968885903066386	27.644269424423157	28.520838619072975
15	16.51437780739777	21.852766392344225	38.00229415989794	23.630561640360064
16	18.663455319270586	21.88349812929485	38.85706526205953	20.595981289375036
17	20.448754828634833	21.64371911192427	29.80799811321429	28.099527946226605
18	21.825608113178554	22.024649711800627	30.9890259111424	25.16071626387842
19	27.21867060223484	23.553374952205004	26.787354247590596	22.440600197969562
20	28.804571167198517	22.978762940384005	23.989336801969692	24.227329090447792
21	21.97140519080478	25.541646863754774	23.749557784599112	28.737390160841336
22	21.89493319420671	22.462040944679302	23.332892606873187	32.3101332542408
23	20.825754624947738	29.10474162113486	24.06723818168174	26.00226557223566
24	25.29114747302933	26.068374541257356	27.91692425341533	20.723553732297983
25	24.142995486722818	24.11833862800662	30.12389178140444	21.614774103866125
26	19.71834005738973	25.88291208221812	34.30197862357553	20.09676923681662
27	18.960409661200465	25.56737575980646	34.93841145507627	20.533803123916798
28	17.141162302879135	24.28236034033612	36.48893478796888	22.087542568815863
29	18.91574143888851	21.54259025661	32.127529561429526	27.41413874307196
30	19.681176096426185	25.37190761896934	26.48075156964133	28.46616471496314
31	21.98748575083708	23.91786764627056	28.069510900832974	26.025135702059384
32	22.6099820969765	24.742621703038512	31.111952858944903	21.535443341040093
33	26.149492032975868	25.363688666063943	27.372329286987966	21.11449001397222
34	23.90893400180817	23.258564684946094	26.1223337538102	26.71016755943554
35	20.08783559235423	23.377560829185146	29.831940280373498	26.702663298087124
36	22.790084369338302	23.361837614931336	33.60908515907247	20.238992856657887
37	20.672810631751602	24.558588627113252	35.078133654468076	19.69046708666707
38	24.097612572853873	25.07924142638141	28.52119596485147	22.30195003591325
39	24.762275720855772	23.699172029831225	29.06614827705733	22.472403972255677
40	20.685675079777447	23.915366225821092	29.337016377157028	26.061942317244437
41	18.892156617507798	23.568383474901818	25.256842278293746	32.28261762929663
42	20.10999103062096	27.15970854878306	24.44566736110863	28.28463305948735
43	20.85684370767686	27.394127379476203	23.955388953012605	27.793639959834334
44	18.86249691789266	28.770623318241427	25.64241837329055	26.72446139057536
45	20.171811850300706	28.2231695855861	28.487605461672878	23.117413102440317
46	21.979266797931682	24.959887936363863	32.0392651541411	21.021580111563352
47	23.644855471499888	23.12992020468766	31.325288288706805	21.89993603510565
48	24.21589402553593	21.345335386880407	28.052000957686683	26.38676962989698
49	21.778081124638636	21.06517629653982	31.181992631530047	25.9747499472915
50	23.775644026429294	23.481905796505874	29.746177293534544	22.996272883530292
51	26.826304937446622	23.64699954617086	23.938951047201805	25.587744469180713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	194.0
2	298.0
3	442.5
4	587.0
5	497.5
6	408.0
7	391.5
8	375.0
9	375.0
10	375.0
11	371.0
12	367.0
13	412.5
14	458.0
15	411.5
16	365.0
17	402.0
18	439.0
19	468.0
20	497.0
21	575.0
22	653.0
23	662.0
24	671.0
25	789.0
26	1196.0
27	1485.0
28	1703.5
29	1922.0
30	2541.0
31	3160.0
32	3724.0
33	4288.0
34	5015.0
35	5742.0
36	6187.5
37	6633.0
38	7628.5
39	8624.0
40	10221.0
41	11818.0
42	13263.5
43	14709.0
44	16983.0
45	19257.0
46	20954.0
47	22651.0
48	23975.0
49	25299.0
50	26749.0
51	28199.0
52	27842.5
53	27486.0
54	27093.5
55	26701.0
56	22951.5
57	19202.0
58	15925.5
59	12649.0
60	11384.5
61	10120.0
62	8873.0
63	7626.0
64	6581.5
65	5537.0
66	4655.0
67	3773.0
68	3306.5
69	2840.0
70	2132.0
71	1424.0
72	1177.0
73	930.0
74	750.0
75	481.5
76	393.0
77	265.5
78	138.0
79	113.0
80	88.0
81	81.5
82	75.0
83	40.0
84	5.0
85	3.0
86	1.0
87	2.0
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.721456112578213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.46535010828092	20.920093910470587
2	9.174229143033928	5.086459811107021
3	3.022842116118387	2.513927551716868
4	1.4256986696916571	1.5808977240647368
5	0.8391770650716717	1.1631605090033268
6	0.6290605341858306	1.0463084394352506
7	0.5452717335258327	1.058100850125607
8	0.41378776941322054	0.9176639591768183
9	0.33386614416829946	0.8329730096733502
>10	7.713725894606579	55.60979270371389
>50	0.39574095080952876	6.495474215715352
>100	0.036093637207383726	1.8235355076632802
>500	0.005156233886769104	0.9516118081339046
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	876	0.3130349019621857	TruSeq Adapter, Index 27 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	649	0.23191741024367407	TruSeq Adapter, Index 27 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	585	0.20904728041995277	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	553	0.1976122155080921	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	476	0.17009659056392737	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	424	0.1515146100821538	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	399	0.14258096561976266	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	280	0.1000568179787808	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14722646074020604	0.0
2	0.0	0.0	0.0	0.9012260533660186	0.0
3	0.0	0.0	0.0	1.2249813286830735	0.0
4	0.0	0.0	0.0	1.603767853888458	0.0
5	3.5734577849564573E-4	0.0	0.0	2.743700887289568	0.0
6	3.5734577849564573E-4	0.0	0.0	3.334750804921366	0.0
7	3.5734577849564573E-4	0.0	0.0	3.9526016559403376	0.0
8	3.5734577849564573E-4	0.0	0.0	5.000696824268067	0.0
9	3.5734577849564573E-4	0.0	0.0	5.357327911206721	0.0
10	3.5734577849564573E-4	0.0	0.0	6.29786200020726	0.0
11	3.5734577849564573E-4	0.0	0.0	7.315225431584364	0.0
12	3.5734577849564573E-4	0.0	0.0	8.324012564277572	0.0
13	3.5734577849564573E-4	0.0	0.0	8.643837036031174	0.0
14	3.5734577849564573E-4	0.0	0.0	8.792135534106867	0.0
15	3.5734577849564573E-4	0.0	0.0	9.04227757905382	0.0
16	3.5734577849564573E-4	0.0	0.0	9.539345556941264	0.0
17	3.5734577849564573E-4	0.0	0.0	10.16255659463767	0.0
18	3.5734577849564573E-4	0.0	0.0	11.008751398115358	0.0
19	3.5734577849564573E-4	0.0	0.0	11.46579664881129	0.0
20	3.5734577849564573E-4	0.0	0.0	11.864237191833935	0.0
21	3.5734577849564573E-4	0.0	0.0	12.358446403493412	0.0
22	3.5734577849564573E-4	0.0	0.0	12.828713447993682	0.0
23	3.5734577849564573E-4	0.0	0.0	13.341504640134934	0.0
24	3.5734577849564573E-4	0.0	0.0	13.725651352017753	0.0
25	3.5734577849564573E-4	0.0	0.0	14.0583402717972	0.0
26	7.146915569912915E-4	0.0	0.0	14.374948631544342	0.0
27	0.0010720373354869371	0.0	0.0	14.709424280216266	0.0
28	0.0010720373354869371	0.0	0.0	15.045686657780669	0.0
29	0.0010720373354869371	0.0	0.0	15.422686454093574	0.0
30	0.0010720373354869371	0.0	0.0	15.82362841756569	0.0
31	0.0010720373354869371	0.0	0.0	16.207060437891517	0.0
32	0.0010720373354869371	0.0	0.0	16.549397693690345	0.0
33	0.0010720373354869371	0.0	0.0	16.933544405573166	0.0
34	0.0010720373354869371	0.0	0.0	17.32698210769687	0.0
35	0.0010720373354869371	0.0	0.0	17.732569566289428	0.0
36	0.0010720373354869371	0.0	0.0	18.11564424083676	0.0
37	0.0010720373354869371	0.0	0.0	18.53516818479065	0.0
38	0.0010720373354869371	0.0	0.0	18.929320578471348	0.0
39	0.0010720373354869371	0.0	0.0	19.25700665735185	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	20	7.0241286E-4	45.000004	34
AATCACG	20	7.0241286E-4	45.000004	1
CATGATA	20	7.0241286E-4	45.000004	40
CCGGCAA	20	7.0241286E-4	45.000004	34
GACGTAG	50	2.1827873E-11	45.000004	1
GAACTCG	30	2.1595952E-6	45.000004	9
CGTTGCC	20	7.0241286E-4	45.000004	22
CCAATGC	25	3.8832164E-5	45.000004	34
GCGTTGT	30	2.1595952E-6	45.000004	44
GCGTTAG	35	1.2075179E-7	45.000004	1
GGCCCAT	25	3.8832164E-5	45.000004	8
GTAATAG	45	3.8198777E-10	45.000004	1
CGTTAGG	45	3.8198777E-10	45.000004	2
TTGAGCG	25	3.8832164E-5	45.000004	1
CACCTAA	20	7.0241286E-4	45.000004	28
AGCATCG	20	7.0241286E-4	45.000004	24
CTACGCG	35	1.2075179E-7	45.000004	1
AACGATG	25	3.8832164E-5	45.000004	21
GTGTAAG	40	6.7830115E-9	45.000004	1
CGGGATC	20	7.0241286E-4	45.000004	6
>>END_MODULE