FastQCFastQC Report
Sat 18 Jun 2016
SRR3550314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307933
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC14060.45659283025853026No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC12500.40593245933368616No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT7540.24485845947007953Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC4340.14093974988065586No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC4030.13087262488918044No Hit
CGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTG3580.11625905635316773No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.11528481845076688No Hit
ACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC3130.10164548781715502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT351.2080636E-745.0000044
CACCGTG302.1603064E-645.00000416
GTCGATG207.0252345E-445.01
TTGTGCG207.0252345E-445.01
CAAGTCG207.0252345E-445.043
CTATAGG207.0252345E-445.01
TTCACGG253.8841317E-545.02
GGATCGC207.0252345E-445.08
TCCTCGA207.0252345E-445.015
CGACAAT253.8841317E-545.020
GTAATAG207.0252345E-445.01
CCCGTCG207.0252345E-445.040
TACGCGG253.8841317E-545.02
TACGATG207.0252345E-445.01
CTACGAA253.8841317E-545.011
ACAGCGT253.8841317E-545.02
GATATCA207.0252345E-445.032
GTCAAGC502.1827873E-1145.016
CGGGATA207.0252345E-445.06
AGCGATT207.0252345E-445.022