##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307933
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25924145836919	33.0	31.0	34.0	30.0	34.0
2	32.35941259949404	34.0	31.0	34.0	30.0	34.0
3	32.3377130739479	34.0	31.0	34.0	30.0	34.0
4	35.90701548713519	37.0	35.0	37.0	35.0	37.0
5	35.95753621729402	37.0	35.0	37.0	35.0	37.0
6	35.96564837156134	37.0	35.0	37.0	35.0	37.0
7	36.17642798920544	37.0	36.0	37.0	35.0	37.0
8	36.094793347903604	37.0	36.0	37.0	35.0	37.0
9	37.90441751939545	39.0	38.0	39.0	35.0	39.0
10	37.60718078283263	39.0	37.0	39.0	35.0	39.0
11	37.46555906642029	39.0	37.0	39.0	35.0	39.0
12	37.24423819467222	39.0	37.0	39.0	35.0	39.0
13	37.144326850321335	39.0	37.0	39.0	33.0	39.0
14	38.32868188859265	40.0	37.0	41.0	34.0	41.0
15	38.49136338099522	40.0	38.0	41.0	35.0	41.0
16	38.47265151834977	40.0	37.0	41.0	35.0	41.0
17	38.457495624048086	40.0	37.0	41.0	34.0	41.0
18	38.430538461288656	40.0	37.0	41.0	34.0	41.0
19	38.35583389893256	40.0	37.0	41.0	34.0	41.0
20	38.33591073382846	40.0	37.0	41.0	34.0	41.0
21	38.11901290215729	40.0	36.0	41.0	34.0	41.0
22	38.1726901631199	40.0	36.0	41.0	34.0	41.0
23	38.1780354817444	40.0	36.0	41.0	34.0	41.0
24	38.17381053670766	40.0	36.0	41.0	34.0	41.0
25	37.90644393423245	40.0	36.0	41.0	34.0	41.0
26	37.900718662826	40.0	36.0	41.0	34.0	41.0
27	37.90780137237646	40.0	36.0	41.0	34.0	41.0
28	37.91038310281782	40.0	36.0	41.0	34.0	41.0
29	37.932917225500354	40.0	36.0	41.0	34.0	41.0
30	37.72815839809309	40.0	36.0	41.0	34.0	41.0
31	37.75420627214361	40.0	36.0	41.0	34.0	41.0
32	37.7047604511371	40.0	35.0	41.0	34.0	41.0
33	37.59291144502213	40.0	35.0	41.0	33.0	41.0
34	37.55422121045812	40.0	35.0	41.0	33.0	41.0
35	37.50184942828472	40.0	35.0	41.0	33.0	41.0
36	37.38301838386922	40.0	35.0	41.0	33.0	41.0
37	37.2992274293434	40.0	35.0	41.0	33.0	41.0
38	37.276589387951276	40.0	35.0	41.0	33.0	41.0
39	37.221918404328214	40.0	35.0	41.0	33.0	41.0
40	37.12672561888463	39.0	35.0	41.0	33.0	41.0
41	37.04495133681677	39.0	35.0	41.0	32.0	41.0
42	37.05185543608512	39.0	35.0	41.0	33.0	41.0
43	36.977836087720384	39.0	35.0	41.0	32.0	41.0
44	36.80998463951574	39.0	35.0	41.0	32.0	41.0
45	36.70975504411674	39.0	35.0	41.0	32.0	41.0
46	36.63050079075643	39.0	35.0	41.0	31.0	41.0
47	36.575199799956486	39.0	35.0	41.0	31.0	41.0
48	36.55062627259826	39.0	35.0	41.0	31.0	41.0
49	36.35345026353135	38.0	35.0	41.0	31.0	41.0
50	36.30634586095027	38.0	35.0	41.0	31.0	41.0
51	35.33763838237539	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	9.0
11	7.0
12	3.0
13	5.0
14	6.0
15	9.0
16	17.0
17	20.0
18	56.0
19	111.0
20	202.0
21	319.0
22	474.0
23	764.0
24	1115.0
25	1512.0
26	1891.0
27	2224.0
28	2534.0
29	3073.0
30	3738.0
31	4736.0
32	6331.0
33	9453.0
34	19871.0
35	29414.0
36	20147.0
37	30601.0
38	57572.0
39	111634.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.88302974997808	20.813293800924228	24.454020842196194	11.849655606901502
2	32.41776620238818	24.37932926967879	25.37597464383486	17.826929884098163
3	31.688386759457416	24.457593047838326	26.65677273952451	17.197247453179752
4	28.913757213419807	25.78710303864802	25.999162155403937	19.299977592528244
5	25.17885384158242	30.525796195925736	24.566382946939758	19.728967015552083
6	27.07666927545927	33.448509903128276	23.779198721799872	15.695622099612578
7	80.09079897250376	6.385479958302617	8.387538847736359	5.136182221457266
8	80.2681102707407	5.504444148564786	8.589206093533333	5.638239487161168
9	74.13041148561538	7.763052352297415	10.78124137393524	7.325294788151967
10	42.691104883205114	24.810267168507437	18.04256120649622	14.456066741791235
11	32.2427281259235	25.097342603748217	23.55317552844288	19.10675374188541
12	29.213822487359266	22.887121549168164	28.164893012441016	19.734162951031557
13	24.133496572306314	26.179720913315556	29.69639499501515	19.99038751936298
14	20.093007245082532	29.990290095572735	28.639022124942766	21.277680534401963
15	19.649079507555214	24.881711281350164	36.128963118600474	19.340246092494144
16	21.48129625600374	24.743694245176712	32.9766540123988	20.798355486420746
17	21.45564132457385	23.973396810345108	27.968746448090982	26.60221541699006
18	22.63966512195835	24.98108354739505	30.02438842215677	22.354862908489835
19	24.123754193282306	26.6499530742077	26.729840582204574	22.496452150305423
20	26.717824981408295	26.428151578427777	26.611957796014067	20.242065644149864
21	24.101671467494555	27.078617751264073	28.041814290771043	20.77789649047033
22	21.687185199377787	25.812757970077904	26.483358392897156	26.01669843764715
23	21.760902533992784	26.45770346146727	27.680372028980333	24.10102197555962
24	23.35475574232057	24.675172846041185	28.636424157203027	23.33364725443522
25	22.534772174466525	26.10600357870056	26.560972679121758	24.79825156771116
26	22.265557767436423	28.063897016558798	27.330945367985894	22.33959984801889
27	22.768914017010193	27.777471073252947	27.732656129742512	21.720958779994348
28	19.721173112332878	27.67712456930566	30.196503784914253	22.40519853344721
29	22.818924896000105	24.994398132061196	28.30226055667954	23.884416415259164
30	22.636092916316212	26.98411667473119	27.36341996473259	23.01637044422001
31	24.765452224996995	26.859738969191348	25.501001841309634	22.87380696450202
32	26.44568786067099	25.62310632507721	25.727025034666635	22.20418077958517
33	23.15568646426333	27.917111839263736	25.02979544251509	23.897406253957843
34	21.344578203700156	25.17787960368002	28.64194483864997	24.835597353969856
35	22.97772567409144	26.35930543332478	26.233628743915073	24.429340148668704
36	23.803229923392426	28.271734435737645	25.705916546781282	22.21911909408865
37	22.276923876297765	27.909967427979467	28.609470241903274	21.2036384538195
38	21.849233437143795	26.887342376426044	26.404769868770156	24.85865431766001
39	22.6244020614874	25.44189807523065	27.404337956633423	24.529361906648525
40	24.702126761340942	23.34501336329656	27.52904040814073	24.423819467221765
41	21.419919268152487	27.008147876323747	24.928799446632873	26.643133408890897
42	23.97534528614991	25.90303734903372	26.636313743574092	23.485303621242284
43	24.180259991621554	25.008037462694805	27.736877827319578	23.07482471836406
44	21.788505941227477	25.53120321628406	28.133067907629254	24.547222934859207
45	22.14345328366885	24.60015652755632	26.755495513634457	26.500894675140373
46	23.265125855299694	25.33668038177136	27.072122831914736	24.326070931014215
47	21.19519505866536	25.70721553065115	29.14919803983334	23.948391370850153
48	22.39026021894373	23.87077708462555	28.087603472183886	25.651359224246832
49	22.26166081582682	23.635661004179482	29.928588361754016	24.174089818239683
50	21.628081433298803	23.667161363023776	28.968964027889182	25.735793175788242
51	20.67754998652304	24.30009125361686	26.05209574810105	28.97026301175905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	101.0
1	191.0
2	281.0
3	551.0
4	821.0
5	645.5
6	470.0
7	483.0
8	496.0
9	540.0
10	584.0
11	581.0
12	578.0
13	597.0
14	616.0
15	606.5
16	597.0
17	631.0
18	665.0
19	636.5
20	608.0
21	653.5
22	699.0
23	781.0
24	863.0
25	1209.5
26	1957.5
27	2359.0
28	2704.0
29	3049.0
30	3799.5
31	4550.0
32	5227.5
33	5905.0
34	6301.0
35	6697.0
36	7127.5
37	7558.0
38	8688.5
39	9819.0
40	11645.5
41	13472.0
42	15772.5
43	18073.0
44	20162.5
45	22252.0
46	24713.0
47	27174.0
48	30239.5
49	33305.0
50	32984.0
51	32663.0
52	28156.5
53	23650.0
54	20087.0
55	16524.0
56	15487.5
57	14451.0
58	13301.5
59	12152.0
60	12207.5
61	12263.0
62	11307.0
63	10351.0
64	8874.5
65	7398.0
66	6127.0
67	4856.0
68	4118.5
69	3381.0
70	2955.0
71	2529.0
72	2114.0
73	1699.0
74	1392.0
75	911.5
76	738.0
77	652.0
78	566.0
79	442.5
80	319.0
81	200.5
82	82.0
83	53.5
84	25.0
85	37.0
86	49.0
87	25.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	307933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.50182822783781
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.714	23.958397659868368
2	10.026	6.517266596246039
3	3.595	3.5053221743723086
4	1.727	2.2452262939790364
5	1.084	1.7615990899488096
6	0.7570000000000007	1.4762330381083948
7	0.637	1.4492565206792882
8	0.541	1.4066791257008207
9	0.459	1.3426505240919802
>10	7.313	50.906963516697814
>50	0.108	2.2598521166815635
>100	0.036000000000000004	2.0622410010563095
>500	0.001	0.24506378483789712
>1k	0.002	0.8632485577313723
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	1406	0.45659283025853026	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	1250	0.40593245933368616	No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT	754	0.24485845947007953	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	434	0.14093974988065586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC	403	0.13087262488918044	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTG	358	0.11625905635316773	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.11528481845076688	No Hit
ACTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	313	0.10164548781715502	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.24745967466949E-4	0.0	0.0	0.2565493142988897	0.0
2	3.24745967466949E-4	0.0	0.0	1.4139439423510958	0.0
3	3.24745967466949E-4	0.0	0.0	1.9302900306235447	0.0
4	3.24745967466949E-4	0.0	0.0	2.483007667252292	0.0
5	3.24745967466949E-4	0.0	0.0	4.152526685999876	0.0
6	3.24745967466949E-4	0.0	0.0	5.100135419068434	0.0
7	3.24745967466949E-4	0.0	0.0	6.114317075467715	0.0
8	3.24745967466949E-4	0.0	0.0	7.747139799891535	0.0
9	3.24745967466949E-4	0.0	0.0	8.408322589654244	0.0
10	3.24745967466949E-4	0.0	0.0	9.778425826397301	0.0
11	3.24745967466949E-4	0.0	0.0	11.525883877336954	0.0
12	3.24745967466949E-4	0.0	0.0	12.781027041596712	0.0
13	3.24745967466949E-4	0.0	0.0	13.299646351641428	0.0
14	3.24745967466949E-4	0.0	0.0	13.482153585357853	0.0
15	3.24745967466949E-4	0.0	0.0	13.854312464074978	0.0
16	3.24745967466949E-4	0.0	0.0	14.644419402922065	0.0
17	3.24745967466949E-4	0.0	0.0	15.612162385973573	0.0
18	3.24745967466949E-4	0.0	0.0	16.797809913195405	0.0
19	3.24745967466949E-4	0.0	0.0	17.379754686896174	0.0
20	3.24745967466949E-4	0.0	0.0	17.97079234768602	0.0
21	3.24745967466949E-4	0.0	0.0	18.618985298750054	0.0
22	3.24745967466949E-4	0.0	0.0	19.28146707238263	0.0
23	3.24745967466949E-4	0.0	0.0	19.96668106373789	0.0
24	3.24745967466949E-4	0.0	0.0	20.533687522935185	0.0
25	6.49491934933898E-4	0.0	0.0	20.988331877388912	0.0
26	6.49491934933898E-4	0.0	0.0	21.444599961679977	0.0
27	6.49491934933898E-4	0.0	0.0	21.886254477435028	0.0
28	6.49491934933898E-4	0.0	0.0	22.32758424722261	0.0
29	6.49491934933898E-4	0.0	0.0	22.76729028717286	0.0
30	6.49491934933898E-4	0.0	0.0	23.342740141524292	0.0
31	0.002273221772268643	0.0	0.0	23.83050858465965	0.0
32	0.002273221772268643	0.0	0.0	24.273462084284567	0.0
33	0.002273221772268643	0.0	0.0	24.72973016857563	0.0
34	0.002273221772268643	0.0	0.0	25.1908694423787	0.0
35	0.002273221772268643	0.0	0.0	25.672467712132185	0.0
36	0.002273221772268643	0.0	0.0	26.12516359078111	0.0
37	0.002273221772268643	0.0	0.0	26.579158453299907	0.0
38	0.002273221772268643	0.0	0.0	27.02795738033923	0.0
39	0.002273221772268643	0.0	0.0	27.50825666622285	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	35	1.2080636E-7	45.000004	4
CACCGTG	30	2.1603064E-6	45.000004	16
GTCGATG	20	7.0252345E-4	45.0	1
TTGTGCG	20	7.0252345E-4	45.0	1
CAAGTCG	20	7.0252345E-4	45.0	43
CTATAGG	20	7.0252345E-4	45.0	1
TTCACGG	25	3.8841317E-5	45.0	2
GGATCGC	20	7.0252345E-4	45.0	8
TCCTCGA	20	7.0252345E-4	45.0	15
CGACAAT	25	3.8841317E-5	45.0	20
GTAATAG	20	7.0252345E-4	45.0	1
CCCGTCG	20	7.0252345E-4	45.0	40
TACGCGG	25	3.8841317E-5	45.0	2
TACGATG	20	7.0252345E-4	45.0	1
CTACGAA	25	3.8841317E-5	45.0	11
ACAGCGT	25	3.8841317E-5	45.0	2
GATATCA	20	7.0252345E-4	45.0	32
GTCAAGC	50	2.1827873E-11	45.0	16
CGGGATA	20	7.0252345E-4	45.0	6
AGCGATT	20	7.0252345E-4	45.0	22
>>END_MODULE