##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	233065
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.251560723403344	33.0	31.0	34.0	30.0	34.0
2	32.347087722309226	34.0	31.0	34.0	30.0	34.0
3	32.32621371720335	34.0	31.0	34.0	30.0	34.0
4	35.90386801965117	37.0	35.0	37.0	35.0	37.0
5	35.94785145774784	37.0	35.0	37.0	35.0	37.0
6	35.95252397399867	37.0	35.0	37.0	35.0	37.0
7	36.1863214124815	37.0	36.0	37.0	35.0	37.0
8	36.11095617102525	37.0	37.0	37.0	35.0	37.0
9	37.920704524488876	39.0	38.0	39.0	35.0	39.0
10	37.549674983373734	39.0	37.0	39.0	35.0	39.0
11	37.49644948834016	39.0	37.0	39.0	35.0	39.0
12	37.440992856070196	39.0	37.0	39.0	35.0	39.0
13	37.40973977216656	39.0	37.0	39.0	35.0	39.0
14	38.63035204771201	40.0	38.0	41.0	35.0	41.0
15	38.743543646622186	40.0	38.0	41.0	35.0	41.0
16	38.722987149507645	40.0	38.0	41.0	35.0	41.0
17	38.71650827022504	40.0	38.0	41.0	35.0	41.0
18	38.66892497801042	40.0	38.0	41.0	35.0	41.0
19	38.59746422671787	40.0	38.0	41.0	35.0	41.0
20	38.596863535923454	40.0	38.0	41.0	35.0	41.0
21	38.39782893184305	40.0	38.0	41.0	34.0	41.0
22	38.47483320103834	40.0	38.0	41.0	34.0	41.0
23	38.45170660545342	40.0	38.0	41.0	34.0	41.0
24	38.44022053933452	40.0	38.0	41.0	34.0	41.0
25	38.179675197906164	40.0	37.0	41.0	34.0	41.0
26	38.20893742089117	40.0	37.0	41.0	34.0	41.0
27	38.257898011284404	40.0	37.0	41.0	34.0	41.0
28	38.236998262287344	40.0	37.0	41.0	34.0	41.0
29	38.262810803853	40.0	37.0	41.0	34.0	41.0
30	38.08754639263725	40.0	37.0	41.0	34.0	41.0
31	38.09535966361315	40.0	37.0	41.0	34.0	41.0
32	38.03363439383863	40.0	37.0	41.0	34.0	41.0
33	37.933057301611136	40.0	37.0	41.0	34.0	41.0
34	37.888503207259774	40.0	37.0	41.0	34.0	41.0
35	37.85483877888143	40.0	37.0	41.0	34.0	41.0
36	37.73555874970502	40.0	37.0	41.0	33.0	41.0
37	37.665861454958915	40.0	36.0	41.0	33.0	41.0
38	37.609709737626844	40.0	36.0	41.0	33.0	41.0
39	37.53913715058031	40.0	36.0	41.0	33.0	41.0
40	37.46747473880677	40.0	36.0	41.0	33.0	41.0
41	37.401647609036104	40.0	36.0	41.0	33.0	41.0
42	37.41737712655268	40.0	36.0	41.0	33.0	41.0
43	37.367880205093	40.0	36.0	41.0	33.0	41.0
44	37.24368309269946	40.0	35.0	41.0	32.0	41.0
45	37.11060004719713	39.0	35.0	41.0	32.0	41.0
46	37.03359148735331	39.0	35.0	41.0	32.0	41.0
47	36.98364833844636	39.0	35.0	41.0	32.0	41.0
48	36.945083989445	39.0	35.0	41.0	32.0	41.0
49	36.77913457619119	39.0	35.0	41.0	31.0	41.0
50	36.72586188402377	39.0	35.0	41.0	31.0	41.0
51	35.75466929826443	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	9.0
11	1.0
12	3.0
13	2.0
14	0.0
15	4.0
16	8.0
17	21.0
18	38.0
19	66.0
20	98.0
21	156.0
22	277.0
23	411.0
24	711.0
25	990.0
26	1376.0
27	1558.0
28	1853.0
29	2166.0
30	2596.0
31	3477.0
32	4393.0
33	6363.0
34	11771.0
35	16871.0
36	15117.0
37	24178.0
38	47370.0
39	91117.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.461974127389354	21.421491858494413	24.47514641838114	11.641387595735095
2	32.11593332332182	24.58327076137558	25.170231480488276	18.13056443481432
3	31.62637032587476	24.270053418574218	26.70628365477442	17.397292600776606
4	28.32643254027846	26.202990582026477	26.400360414476648	19.070216463218415
5	24.913221633449893	30.630081736854525	25.005041512024544	19.451655117671034
6	26.589148949863773	33.640829811426	24.653208332439448	15.116812906270782
7	81.69523523480574	5.384763907064553	8.121339540471542	4.798661317658164
8	81.76646000042906	4.657498980970974	8.03552656984103	5.5405144487589295
9	74.95505545663227	7.582005020058783	10.759659322506597	6.703280200802351
10	36.67174393409564	29.057988114903566	18.73640400746573	15.533863943535064
11	28.14922875592646	26.428249629931567	26.24246454851651	19.18005706562547
12	26.018492695170874	23.810095895994678	30.007079570077018	20.16433183875743
13	23.67880205093	26.06011198592667	31.507519361551502	18.75356660159183
14	20.37628987621479	29.474610087314694	29.047261493574755	21.10183854289576
15	19.77474095209491	27.073134104219854	33.72878810632227	19.423336837362967
16	21.766030935575913	26.57756419882865	31.318730826164376	20.33767403943106
17	22.09812713191599	25.85158646729453	27.970737777014996	24.079548623774482
18	23.449681419346536	25.246605024349428	30.787977602814664	20.51573595348937
19	22.672215905434108	27.64722287773797	28.08229463883466	21.598266577993265
20	24.43309806277219	27.471306287945424	27.837298607684552	20.258297041597835
21	23.473279986269922	28.06556110956171	28.410100186643213	20.05105871752515
22	22.30493639113552	25.69669405530646	27.261493574753825	24.736875978804196
23	20.651320447085578	27.975457490399673	28.17154012829039	23.201681934224357
24	21.9741273893549	25.584708128633643	29.84789650955742	22.593267972454036
25	20.75257975242958	28.172398257996694	27.691845622465838	23.38317636710789
26	20.338961233990517	28.32385815115955	29.17426469010791	22.162915924742023
27	21.676356381267027	27.622337116255125	27.992191019672624	22.709115482805224
28	18.807628773089053	28.866196125544374	29.521807221161477	22.804367880205092
29	19.847681977130843	27.17439340956386	29.344603436809475	23.633321176495826
30	21.49185849441143	26.672387531375367	27.288524660502432	24.547229313710766
31	21.28676549460451	28.716452491794136	25.265054813034993	24.731727200566368
32	21.896037586081135	29.252783558234828	25.94640979984125	22.90476905584279
33	20.718254564177375	28.310128075858664	25.05180958101817	25.919807778945785
34	19.374852508956728	27.875485379615128	27.67296676892712	25.076695342501022
35	20.554780855126253	26.84916225087422	27.155943620878297	25.440113273121234
36	20.911762812949178	29.36734387402656	25.544376032437306	24.17651728058696
37	20.276317765430242	29.240769742346558	26.684401347263638	23.798511144959562
38	20.304636045738313	30.525818977538457	25.159075794306307	24.010469182416923
39	21.425353442172785	27.873769120202518	26.07298393152125	24.627893506103447
40	22.93780704953554	25.021345976444337	27.926544097140283	24.11430287687984
41	20.029605474867527	27.30139660609701	26.690408255207775	25.97858966382769
42	21.91191298564778	26.752622658914895	27.48117477956793	23.85428957586939
43	21.34554737948641	26.781799068929267	27.936841653615947	23.935811897968378
44	20.909617488683416	27.493188595456203	26.883058374273272	24.714135541587112
45	20.612275545448693	25.855018986119756	26.280651320447085	27.252054147984467
46	22.076244824405208	26.36217364254607	26.715723081543775	24.845858451504945
47	20.075086349301696	26.196983674082336	28.91725484306953	24.810675133546436
48	20.51230343466415	25.711282260313645	28.108038530023816	25.668375774998392
49	21.20438504279922	24.739450367923112	29.414970072726494	24.641194516551177
50	20.05706562546929	24.87031514813464	29.04811962328106	26.024499603115007
51	20.25186106880055	24.615879690215177	26.030935575912302	29.101323665071977
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	101.0
1	147.5
2	194.0
3	437.0
4	680.0
5	519.0
6	358.0
7	344.5
8	331.0
9	371.0
10	411.0
11	418.0
12	425.0
13	409.5
14	394.0
15	410.5
16	427.0
17	457.5
18	488.0
19	485.0
20	482.0
21	519.5
22	557.0
23	706.5
24	856.0
25	1047.0
26	1600.5
27	1963.0
28	2271.5
29	2580.0
30	2778.0
31	2976.0
32	3886.5
33	4797.0
34	5289.5
35	5782.0
36	6520.5
37	7259.0
38	7818.5
39	8378.0
40	9733.0
41	11088.0
42	12833.0
43	14578.0
44	16357.5
45	18137.0
46	20337.5
47	22538.0
48	24927.5
49	27317.0
50	26684.0
51	26051.0
52	22458.0
53	18865.0
54	16248.0
55	13631.0
56	11880.0
57	10129.0
58	9175.5
59	8222.0
60	7634.0
61	7046.0
62	6156.5
63	5267.0
64	4359.0
65	3451.0
66	2812.0
67	2173.0
68	1803.0
69	1433.0
70	1163.0
71	893.0
72	772.5
73	652.0
74	514.5
75	342.0
76	307.0
77	218.5
78	130.0
79	94.0
80	58.0
81	41.0
82	24.0
83	20.5
84	17.0
85	9.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	233065.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.645745522270396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.57323766002301	31.238673171143443
2	9.788207571692325	8.544272335864871
3	4.11953719855007	5.3940081671232925
4	2.760442425413356	4.819262705138809
5	2.0295817821248208	4.429130498962798
6	1.743640380770678	4.5661490604883
7	1.4686970744349537	4.487166513206371
8	1.2007523388172638	4.192618481223144
9	1.034786570070193	4.0647628176432
>10	4.222129164640438	24.912654215982588
>50	0.03799216392525108	1.0673547881854064
>100	0.017996288175118936	1.3645210231626188
>500	0.0029993813625198225	0.919426221875164
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	802	0.3441100122283483	No Hit
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	772	0.3312380666337717	No Hit
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	533	0.2286915667303113	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	316	0.13558449359620706	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	278	0.11928002917641002	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	275	0.11799283461695236	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	243	0.10426275931607062	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2381309934996675	0.0
2	0.0	0.0	0.0	1.1915130972046424	0.0
3	0.0	0.0	0.0	1.7179756720228263	0.0
4	0.0	0.0	0.0	2.2937807049535537	0.0
5	0.0	0.0	0.0	3.955977946066548	0.0
6	0.0	0.0	0.0	5.007615901143458	0.0
7	0.0	0.0	0.0	6.012914852079892	0.0
8	0.0	0.0	0.0	7.736468367193702	0.0
9	0.0	0.0	0.0	8.467165812112501	0.0
10	0.0	0.0	0.0	9.956449917405015	0.0
11	0.0	0.0	0.0	11.72290991783408	0.0
12	0.0	0.0	0.0	13.125093857936626	0.0
13	0.0	0.0	0.0	13.692746658657455	0.0
14	0.0	0.0	0.0	13.919292901122004	0.0
15	0.0	0.0	0.0	14.312745371462897	0.0
16	0.0	0.0	0.0	15.14126960290048	0.0
17	0.0	0.0	0.0	16.174028704438676	0.0
18	0.0	0.0	0.0	17.32006092720915	0.0
19	0.0	0.0	0.0	17.999270589749642	0.0
20	0.0	0.0	0.0	18.684916225087424	0.0
21	0.0	0.0	0.0	19.483405916804326	0.0
22	0.0	0.0	0.0	20.32222770471757	0.0
23	0.0	0.0	0.0	21.17949928131637	0.0
24	0.0	0.0	0.0	21.85785081415056	0.0
25	0.0	0.0	0.0	22.440091819878575	0.0
26	0.0	0.0	0.0	22.967412524403063	0.0
27	0.0	0.0	0.0	23.534207195417586	0.0
28	0.0	0.0	0.0	24.113444747173535	0.0
29	0.0	0.0	0.0	24.701263595992533	0.0
30	0.0	0.0	0.0	25.43153197605818	0.0
31	0.0	0.0	0.0	26.068264218136573	0.0
32	0.0	0.0	0.0	26.64278205650784	0.0
33	0.0	0.0	0.0	27.25119601827816	0.0
34	0.0	0.0	0.0	27.813270975908008	0.0
35	0.0	0.0	0.0	28.394224787076567	0.0
36	4.29064853152554E-4	0.0	0.0	28.978611117070344	0.0
37	4.29064853152554E-4	0.0	0.0	29.56557183618304	0.0
38	4.29064853152554E-4	0.0	0.0	30.143093128526377	0.0
39	4.29064853152554E-4	0.0	0.0	30.730911977345375	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	20	7.021702E-4	45.000004	6
GGGACGT	20	7.021702E-4	45.000004	7
TACGAAT	30	2.1580308E-6	45.000004	12
CGGGCTA	20	7.021702E-4	45.000004	6
CGAATAT	30	2.1580308E-6	45.000004	14
GACGGGT	35	1.2063538E-7	45.000004	4
CATACGA	20	7.021702E-4	45.000004	18
CACGTGA	30	2.1580308E-6	45.000004	43
AATACGG	20	7.021702E-4	45.000004	2
AGGGCGT	25	3.8812075E-5	45.0	6
ACGGGAC	80	0.0	42.187504	5
AACGGGA	50	1.0732037E-9	40.5	4
CGTTTTT	390	0.0	40.384617	1
GACGGGA	95	0.0	40.263157	4
AGCGGGC	90	0.0	40.0	4
AATGGGC	85	0.0	39.705883	4
TTAGCGG	40	3.4424193E-7	39.375004	2
TACGGGA	80	0.0	39.375004	4
TATTAGG	40	3.4424193E-7	39.375004	2
CGGGTAT	35	6.2268737E-6	38.57143	6
>>END_MODULE