SRR3550312_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	115973
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	402	0.3466324058185957	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	272	0.2345373492105921	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	188	0.1621066972484975	Illumina PCR Primer Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	166	0.14313676459175842	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	139	0.1198554836039423	Illumina PCR Primer Index 10 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	125	0.10778370827692653	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGATGG	30	2.1485976E-6	45.000004	2
CGGGATG	35	1.1993325E-7	45.000004	6
TAGGGTC	30	2.1485976E-6	45.000004	5
TTTATGG	30	2.1485976E-6	45.000004	2
TAAGGGA	35	1.1993325E-7	45.000004	4
CGATAGG	30	2.1485976E-6	45.000004	2
TTGGGCA	35	1.1993325E-7	45.000004	5
CTTCGGA	20	7.007047E-4	45.0	13
GTTTGCC	25	3.869073E-5	45.0	25
GGAATCG	20	7.007047E-4	45.0	8
ACTATGA	25	3.869073E-5	45.0	25
GGGTGCG	20	7.007047E-4	45.0	9
TCTGAGG	25	3.869073E-5	45.0	2
CCGGGAT	20	7.007047E-4	45.0	5
ACACGGG	25	3.869073E-5	45.0	3
CGTGCGT	25	3.869073E-5	45.0	17
TGGACGG	25	3.869073E-5	45.0	1
TTTCGCA	20	7.007047E-4	45.0	14
AACTTCG	20	7.007047E-4	45.0	11
AACCAGC	20	7.007047E-4	45.0	23