##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550312_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	115973
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25339518681072	33.0	31.0	34.0	30.0	34.0
2	32.365602338475334	34.0	31.0	34.0	30.0	34.0
3	32.360851232614486	34.0	31.0	34.0	30.0	34.0
4	35.92895760220051	37.0	35.0	37.0	35.0	37.0
5	35.93883058987868	37.0	35.0	37.0	35.0	37.0
6	35.943900735516024	37.0	35.0	37.0	35.0	37.0
7	36.13792865580782	37.0	35.0	37.0	35.0	37.0
8	36.06454088451623	37.0	36.0	37.0	35.0	37.0
9	37.876385020651355	39.0	38.0	39.0	35.0	39.0
10	37.556008726169026	39.0	37.0	39.0	35.0	39.0
11	37.38821967182017	39.0	37.0	39.0	35.0	39.0
12	37.01010580048804	39.0	35.0	39.0	34.0	39.0
13	36.77923309735887	39.0	35.0	39.0	33.0	39.0
14	37.91459218956136	40.0	36.0	41.0	33.0	41.0
15	38.1244686263182	40.0	36.0	41.0	33.0	41.0
16	38.173229975942675	40.0	36.0	41.0	34.0	41.0
17	38.17048795840411	40.0	36.0	41.0	34.0	41.0
18	38.16304657118467	40.0	36.0	41.0	34.0	41.0
19	38.02643718796617	40.0	36.0	41.0	34.0	41.0
20	38.000672570339646	40.0	35.0	41.0	34.0	41.0
21	37.75209747096307	40.0	35.0	41.0	34.0	41.0
22	37.82209652246644	40.0	35.0	41.0	34.0	41.0
23	37.851586145051	40.0	35.0	41.0	34.0	41.0
24	37.81391358333405	40.0	35.0	41.0	34.0	41.0
25	37.495813680770524	39.0	35.0	41.0	33.0	41.0
26	37.42316746139188	39.0	35.0	41.0	33.0	41.0
27	37.509204728686846	39.0	35.0	41.0	33.0	41.0
28	37.55061954075518	39.0	35.0	41.0	33.0	41.0
29	37.59959645779621	39.0	35.0	41.0	34.0	41.0
30	37.44653496934632	39.0	35.0	41.0	33.0	41.0
31	37.41970113733369	39.0	35.0	41.0	33.0	41.0
32	37.34479577142956	39.0	35.0	41.0	33.0	41.0
33	37.2991041018168	39.0	35.0	41.0	33.0	41.0
34	37.245126020711716	39.0	35.0	41.0	33.0	41.0
35	37.1850948065498	39.0	35.0	41.0	33.0	41.0
36	37.076233261190104	39.0	35.0	41.0	33.0	41.0
37	36.976442792719	39.0	35.0	41.0	33.0	41.0
38	36.91871383856587	39.0	35.0	41.0	33.0	41.0
39	36.85177584437757	39.0	35.0	41.0	32.0	41.0
40	36.7347744733688	39.0	35.0	41.0	32.0	41.0
41	36.69473067006976	39.0	35.0	41.0	32.0	41.0
42	36.69610167883904	39.0	35.0	41.0	32.0	41.0
43	36.62181714709458	38.0	35.0	41.0	32.0	41.0
44	36.47414484405853	38.0	35.0	41.0	32.0	41.0
45	36.33802695454977	38.0	35.0	40.0	31.0	41.0
46	36.27779741836462	38.0	35.0	40.0	31.0	41.0
47	36.188621489484625	38.0	35.0	40.0	31.0	41.0
48	36.18304260474421	38.0	35.0	40.0	31.0	41.0
49	36.06536866339579	37.0	35.0	40.0	31.0	41.0
50	35.95797297646866	37.0	35.0	40.0	31.0	41.0
51	34.987040086916785	36.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	1.0
12	2.0
13	1.0
14	5.0
15	1.0
16	15.0
17	16.0
18	29.0
19	57.0
20	104.0
21	121.0
22	202.0
23	319.0
24	432.0
25	608.0
26	731.0
27	821.0
28	997.0
29	1155.0
30	1482.0
31	1910.0
32	2624.0
33	3995.0
34	8942.0
35	14845.0
36	7811.0
37	11428.0
38	20428.0
39	36863.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29323204538988	21.80766212825399	25.954316953083907	10.944788873272227
2	29.970769058315298	25.719779603873317	26.248350909263362	18.061100428548023
3	30.768368499564552	25.27743526510481	26.69759340536159	17.256602829969044
4	28.265199658541214	25.787036637838117	26.627749562398144	19.320014141222526
5	25.242082208789977	29.70174092245609	24.87647987031464	20.179696998439294
6	26.36561958386866	34.93140644805256	23.088132582583878	15.614841385494898
7	82.04840781906132	6.469609305614238	7.276693713191864	4.205289162132566
8	83.22454364377916	4.712303725867228	6.458399799953438	5.604752830400179
9	78.0069498935097	6.504962361929071	9.234908125167065	6.25317961939417
10	50.569529114535285	19.79253791830857	14.712907314633577	14.925025652522569
11	38.07351711174153	20.3590490890121	20.56513153923758	21.002302260008797
12	31.633224974778614	20.209014167090615	28.40488734446811	19.752873513662664
13	22.57594440085192	28.345390737499244	28.99554206582567	20.083122795823165
14	17.207453458994763	31.021013511765673	27.721969768825506	24.04956326041406
15	15.799367094064998	22.78547592974227	41.125089460477874	20.290067515714867
16	17.977460270925132	20.9747096306899	39.316047700757935	21.731782397627033
17	19.027704724375504	20.34007915635536	29.22318125770654	31.409034861562606
18	22.135324601415846	21.39032360980573	31.97123468393505	24.50311710484337
19	26.487199606805035	23.721038517585992	25.931035672096094	23.860726203512886
20	28.01772826433739	23.05967768359877	24.327214092935424	24.59537995912842
21	23.034671863278522	26.63033637139679	25.485242254662722	24.849749510661965
22	21.507592284411025	23.49684840436998	24.039216024419478	30.956343286799516
23	20.069326481163717	28.591999862036854	24.84716270166332	26.49151095513611
24	23.370094763436317	23.843480810188577	30.381209419433837	22.40521500694127
25	20.714304191492847	23.842618540522363	30.839936881860435	24.603140386124355
26	18.355996654393696	27.971165702361755	30.45708915006079	23.215748493183757
27	20.22453502108249	26.94075345123434	30.221689531183983	22.613021996499185
28	17.75327015770912	25.46196097367491	34.32781768170178	22.456951186914196
29	18.49999568865167	22.96569028998129	31.693583851413692	26.840730169953353
30	19.949470997559775	25.071352814879326	28.569580850715255	26.409595336845644
31	23.55548274167263	27.041639002181544	24.334112250265147	25.068766005880676
32	25.778413941175966	25.35331499573176	26.14142947065265	22.72684159243962
33	24.87475533098221	24.98081449992671	24.542781509489277	25.601648659601807
34	20.545299336914628	25.990532279064954	26.569115225095498	26.89505315892492
35	20.035697964181317	23.08209669492037	30.357065868779802	26.525139472118507
36	25.05755650021988	22.61043518750054	29.16196011140524	23.17004820087434
37	21.669698981659526	27.04336354151397	30.59074094832418	20.696196528502323
38	23.096755279246032	27.693514869840396	26.242315021599854	22.96741482931372
39	21.68177075698654	25.7077078285463	28.51180878307882	24.09871263138834
40	24.0236951704276	23.114000672570338	26.533762168780665	26.3285419882214
41	18.338751261069387	22.684590378795065	24.238400317315236	34.73825804282031
42	22.326748467315667	25.14550800617385	23.755529304234607	28.772214222275878
43	22.343131590973762	24.474662205858262	25.094634095867143	28.08757210730084
44	20.38836625766342	28.297103636191185	26.607917360075188	24.706612746070206
45	19.010459331051194	28.45144990644374	27.67023358885258	24.86785717365249
46	23.47270485371595	26.432014348167243	28.93690772852302	21.158373069593786
47	21.72315970096488	24.26857975563278	29.293025100669983	24.71523544273236
48	24.17631690134773	20.84968052908867	28.01772826433739	26.95627430522622
49	22.16895311839825	19.90204616591793	31.335741939934298	26.59325877574953
50	22.562148086192476	24.172867822682868	29.134367482086347	24.13061660903831
51	22.159468152069877	25.962077380079844	23.567554516999646	28.310899950850626
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	42.0
1	66.5
2	91.0
3	154.5
4	218.0
5	183.0
6	148.0
7	155.5
8	163.0
9	179.0
10	195.0
11	204.0
12	213.0
13	222.0
14	231.0
15	218.5
16	206.0
17	205.0
18	204.0
19	199.5
20	195.0
21	201.0
22	207.0
23	233.0
24	259.0
25	357.0
26	499.5
27	544.0
28	578.0
29	612.0
30	808.0
31	1004.0
32	1218.0
33	1432.0
34	1749.5
35	2067.0
36	2275.0
37	2483.0
38	2769.5
39	3056.0
40	3816.0
41	4576.0
42	6009.0
43	7442.0
44	8549.5
45	9657.0
46	10334.5
47	11012.0
48	11529.5
49	12047.0
50	11809.5
51	11572.0
52	10993.0
53	10414.0
54	9086.5
55	7759.0
56	6881.5
57	6004.0
58	5508.0
59	5012.0
60	4602.5
61	4193.0
62	3725.5
63	3258.0
64	2950.5
65	2643.0
66	2374.0
67	2105.0
68	1770.5
69	1436.0
70	1308.5
71	1181.0
72	960.5
73	740.0
74	581.5
75	320.5
76	218.0
77	190.0
78	162.0
79	111.5
80	61.0
81	36.0
82	11.0
83	6.5
84	2.0
85	11.0
86	20.0
87	10.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	115973.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.69826597570124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1690290048518	31.813439335017634
2	9.413335028284497	7.6621282539901525
3	2.8051441767834064	3.4249351142076176
4	1.3029937074938027	2.121183378889914
5	0.7712027797199092	1.56933079251205
6	0.5678086400135596	1.386529623274383
7	0.5211974829975211	1.4848283652229397
8	0.4067882794126994	1.3244462073068732
9	0.3919574567257781	1.4356789942486614
>10	5.608169664611538	45.87447078199236
>50	0.029661645373842666	0.7889767445871021
>100	0.012712133731646856	1.1140524087503125
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	402	0.3466324058185957	Illumina PCR Primer Index 10 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	272	0.2345373492105921	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	188	0.1621066972484975	Illumina PCR Primer Index 10 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	166	0.14313676459175842	No Hit
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	139	0.1198554836039423	Illumina PCR Primer Index 10 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	125	0.10778370827692653	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17331620290929786	0.0
2	0.0	0.0	0.0	1.001957352142309	0.0
3	0.0	0.0	0.0	1.3761823872797978	0.0
4	0.0	0.0	0.0	1.8150776473834427	0.0
5	0.0	0.0	0.0	3.056745966733636	0.0
6	0.0	0.0	0.0	3.8914230036301554	0.0
7	0.0	0.0	0.0	4.677812939218612	0.0
8	0.0	0.0	0.0	6.4644356876169455	0.0
9	0.0	0.0	0.0	7.184430858906815	0.0
10	0.0	0.0	0.0	8.462314504238055	0.0
11	0.0	0.0	0.0	9.735024531572003	0.0
12	0.0	0.0	0.0	10.861148715649332	0.0
13	0.0	0.0	0.0	11.239685099117898	0.0
14	0.0	0.0	0.0	11.374199167047502	0.0
15	0.0	0.0	0.0	11.638915954575634	0.0
16	0.0	0.0	0.0	12.279582316573686	0.0
17	0.0	0.0	0.0	13.060798634164849	0.0
18	0.0	0.0	0.0	14.255042121873194	0.0
19	0.0	0.0	0.0	14.671518370655239	0.0
20	0.0	0.0	0.0	15.087994619437282	0.0
21	0.0	0.0	0.0	15.513955834547696	0.0
22	0.0	0.0	0.0	15.975270105972943	0.0
23	0.0	0.0	0.0	16.449518422391417	0.0
24	0.0	0.0	0.0	16.841851120519433	0.0
25	0.0	0.0	0.0	17.154855009355625	0.0
26	0.0	0.0	0.0	17.4609607408621	0.0
27	0.0	0.0	0.0	17.74809653971183	0.0
28	0.0	0.0	0.0	18.030920990230484	0.0
29	0.0	0.0	0.0	18.34047580040182	0.0
30	0.0	0.0	0.0	18.719874453536598	0.0
31	8.622696662154122E-4	0.0	0.0	19.028566994041718	0.0
32	8.622696662154122E-4	0.0	0.0	19.335534995214402	0.0
33	8.622696662154122E-4	0.0	0.0	19.620083985065488	0.0
34	8.622696662154122E-4	0.0	0.0	19.90808205358144	0.0
35	8.622696662154122E-4	0.0	0.0	20.265061695394618	0.0
36	8.622696662154122E-4	0.0	0.0	20.56944288756866	0.0
37	8.622696662154122E-4	0.0	0.0	20.895380821398085	0.0
38	8.622696662154122E-4	0.0	0.0	21.214420597897785	0.0
39	8.622696662154122E-4	0.0	0.0	21.54122080139343	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGATGG	30	2.1485976E-6	45.000004	2
CGGGATG	35	1.1993325E-7	45.000004	6
TAGGGTC	30	2.1485976E-6	45.000004	5
TTTATGG	30	2.1485976E-6	45.000004	2
TAAGGGA	35	1.1993325E-7	45.000004	4
CGATAGG	30	2.1485976E-6	45.000004	2
TTGGGCA	35	1.1993325E-7	45.000004	5
CTTCGGA	20	7.007047E-4	45.0	13
GTTTGCC	25	3.869073E-5	45.0	25
GGAATCG	20	7.007047E-4	45.0	8
ACTATGA	25	3.869073E-5	45.0	25
GGGTGCG	20	7.007047E-4	45.0	9
TCTGAGG	25	3.869073E-5	45.0	2
CCGGGAT	20	7.007047E-4	45.0	5
ACACGGG	25	3.869073E-5	45.0	3
CGTGCGT	25	3.869073E-5	45.0	17
TGGACGG	25	3.869073E-5	45.0	1
TTTCGCA	20	7.007047E-4	45.0	14
AACTTCG	20	7.007047E-4	45.0	11
AACCAGC	20	7.007047E-4	45.0	23
>>END_MODULE