SRR3550311_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663706
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5241	0.7896568661425389	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1692	0.2549321536945576	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1611	0.24272795484747764	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1287	0.1939111594591581	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	895	0.13484886380415426	Illumina PCR Primer Index 7 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	813	0.12249399583550548	Illumina PCR Primer Index 7 (96% over 25bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	40	6.8084773E-9	45.000004	44
TCGGACG	20	7.031129E-4	45.000004	1
CGTAAGC	20	7.031129E-4	45.000004	27
GTATACG	30	2.16411E-6	45.000004	1
TGTAGCG	30	2.16411E-6	45.000004	1
TATAGCG	25	3.889019E-5	45.0	1
CGTTTTT	4980	0.0	44.051205	1
GCGTTAG	50	1.0804797E-9	40.5	1
TTGCACG	50	1.0804797E-9	40.5	1
CGATAGT	50	1.0804797E-9	40.5	10
TATACGG	75	0.0	39.0	2
TACGGGA	255	0.0	38.82353	4
GTCGATG	35	6.2443014E-6	38.571426	1
CGACAGG	70	0.0	38.571426	2
AACACGT	140	0.0	38.571426	41
ACGACCA	160	0.0	37.968754	28
TTAGTCG	30	1.139402E-4	37.500004	1
TAATGCG	30	1.139402E-4	37.500004	1
AGTAACG	30	1.139402E-4	37.500004	1
CACGACC	165	0.0	36.81818	27