##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550311_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	663706
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.35270436006304	33.0	31.0	34.0	31.0	34.0
2	32.49793131296086	34.0	31.0	34.0	31.0	34.0
3	32.38840239503636	34.0	31.0	34.0	30.0	34.0
4	35.995446779146185	37.0	35.0	37.0	35.0	37.0
5	36.04460258005804	37.0	35.0	37.0	35.0	37.0
6	36.049122653705105	37.0	35.0	37.0	35.0	37.0
7	36.24059749346849	37.0	37.0	37.0	35.0	37.0
8	36.17640340753285	37.0	37.0	37.0	35.0	37.0
9	38.007096515625896	39.0	38.0	39.0	35.0	39.0
10	37.6593325960591	39.0	37.0	39.0	35.0	39.0
11	37.59825736093993	39.0	37.0	39.0	35.0	39.0
12	37.54960178151169	39.0	37.0	39.0	35.0	39.0
13	37.56643453577337	39.0	37.0	39.0	35.0	39.0
14	38.89802412513975	40.0	38.0	41.0	35.0	41.0
15	38.99236408891889	40.0	38.0	41.0	35.0	41.0
16	38.934136500197376	40.0	38.0	41.0	35.0	41.0
17	38.90429497397944	40.0	38.0	41.0	35.0	41.0
18	38.75660458094397	40.0	38.0	41.0	35.0	41.0
19	38.577707599449155	40.0	37.0	41.0	35.0	41.0
20	38.47863813194396	40.0	37.0	41.0	35.0	41.0
21	38.267368382988856	40.0	37.0	41.0	34.0	41.0
22	38.32921805739288	40.0	37.0	41.0	34.0	41.0
23	38.29958596125393	40.0	37.0	41.0	34.0	41.0
24	38.25275046481424	40.0	36.0	41.0	34.0	41.0
25	38.01057998571657	40.0	36.0	41.0	34.0	41.0
26	37.98736338077402	40.0	36.0	41.0	34.0	41.0
27	38.01511512627579	40.0	36.0	41.0	34.0	41.0
28	37.964255257599	40.0	36.0	41.0	34.0	41.0
29	37.925909966159715	40.0	36.0	41.0	34.0	41.0
30	37.6916872832248	40.0	36.0	41.0	33.0	41.0
31	37.615549656022395	40.0	36.0	41.0	33.0	41.0
32	37.387572208176515	40.0	36.0	41.0	33.0	41.0
33	37.11893519118405	40.0	36.0	41.0	32.0	41.0
34	36.84853383877801	40.0	35.0	41.0	31.0	41.0
35	36.691886166465274	40.0	35.0	41.0	30.0	41.0
36	36.55780722187234	40.0	35.0	41.0	30.0	41.0
37	36.49070220850798	40.0	35.0	41.0	30.0	41.0
38	36.403487387487836	40.0	35.0	41.0	30.0	41.0
39	36.31530828408964	40.0	35.0	41.0	29.0	41.0
40	36.23181951044589	40.0	35.0	41.0	29.0	41.0
41	36.13224228800101	40.0	35.0	41.0	28.0	41.0
42	36.09612388617852	40.0	35.0	41.0	28.0	41.0
43	36.04297083347144	39.0	35.0	41.0	28.0	41.0
44	35.8992008509792	39.0	35.0	41.0	27.0	41.0
45	35.792466845259796	39.0	35.0	41.0	26.0	41.0
46	35.72655061126463	39.0	35.0	41.0	26.0	41.0
47	35.68147191678243	39.0	35.0	41.0	26.0	41.0
48	35.59775713945633	39.0	35.0	41.0	26.0	41.0
49	35.42574121674356	39.0	35.0	41.0	24.0	41.0
50	35.3789659879525	39.0	35.0	41.0	24.0	41.0
51	34.46679855237108	38.0	34.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	19.0
10	18.0
11	23.0
12	14.0
13	13.0
14	16.0
15	27.0
16	34.0
17	87.0
18	175.0
19	333.0
20	592.0
21	930.0
22	1500.0
23	2395.0
24	3988.0
25	7096.0
26	10021.0
27	11077.0
28	10145.0
29	9107.0
30	9227.0
31	10276.0
32	12382.0
33	16615.0
34	31084.0
35	42026.0
36	41223.0
37	62184.0
38	121006.0
39	259845.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.884234887133765	19.399101409358963	23.99435894808846	17.722304755418815
2	37.363832781382115	22.74591460676866	24.424368621046067	15.465883990803157
3	30.40427538699364	22.685345619897966	31.31386487390501	15.596514119203384
4	26.24415027135509	25.168071405110094	31.183234745504784	17.404543578030033
5	23.854990010637238	29.18249948019153	29.390422867956595	17.572087641214633
6	24.296149198590943	33.7057371788111	28.144238563460327	13.85387505913763
7	78.28541553037037	5.315455939828779	12.418902345315546	3.980226184485299
8	80.64007256224895	4.297234016266238	11.786393372969357	3.276300048515457
9	74.4130684369284	7.04332942598078	13.631186097458814	4.9124160396320065
10	35.4467188785396	28.067397311460198	21.90879696733192	14.57708684266829
11	27.340720138133452	24.70822924608185	30.40307003402109	17.54798058176361
12	23.84805923104507	23.39740788843253	33.28280895456723	19.471723925955168
13	22.161318415081375	23.224741075114583	35.9517617740385	18.662178735765536
14	20.639409618114044	26.65818901742639	33.51649676212058	19.185904602338987
15	20.71429217153378	25.31738450458486	35.463895158398444	18.50442816548291
16	23.396051866338407	24.90455111148611	33.69564234766598	18.003754674509498
17	23.55335042925633	24.24763374144576	32.211551500212444	19.98746432908547
18	23.499862891099372	24.283342323257585	32.54362624415027	19.67316854149277
19	23.051019577945656	26.039842942507676	31.593808101780006	19.315329377766663
20	24.33562450844199	25.797717664146475	30.76271722720602	19.10394060020551
21	23.419254911060015	25.936031917746718	31.552072755105424	19.092640416087846
22	23.367726071483457	24.489156343320687	31.390555456783574	20.75256212841228
23	21.94842294630454	26.4639765197241	31.457301877638592	20.130298656332773
24	21.30220308389558	25.345408961196675	32.82070675871546	20.531681196192288
25	22.110090913747953	26.419378459739708	30.704709615402	20.76582101111034
26	21.691532094029586	27.18086020014886	30.77793480848448	20.349672897337072
27	21.46040566154291	26.370410995229815	31.161990399363575	21.007192943863696
28	21.205322838726786	26.7671227923207	31.6388581691291	20.388696199823418
29	21.571900811503887	26.316772787951294	31.321850337348163	20.78947606319666
30	22.426797407285758	26.028994765754714	30.842270523394394	20.70193730356513
31	22.906377221239524	26.894438200046405	29.395093610725233	20.804090967988838
32	22.8164277556629	27.816834562291138	28.90240558319497	20.464332098851
33	23.204250074581214	26.980018261097534	28.797389205461453	21.018342458859795
34	21.429819829864428	28.487010815029546	28.721903975555442	21.361265379550584
35	22.135252657049957	28.07914950294257	28.85991689091254	20.92568094909493
36	21.861034855794585	28.975329438034308	28.056398465585662	21.107237240585437
37	22.659731869231255	28.957399812567612	27.70759944915369	20.67526886904744
38	21.81658746493176	28.52166471299039	28.220326469852612	21.44142135222523
39	22.326903779685583	27.392399646831578	27.874239497608883	22.406457075873956
40	22.660183876595962	26.776765616101105	29.455963935839062	21.10708657146387
41	21.124564189565863	27.37055262420409	29.39418959599582	22.110693590234227
42	21.872937716398525	27.61538994675353	28.529047499947264	21.982624836900676
43	22.28788047719924	26.558446058947787	28.967795981955867	22.185877481897105
44	21.84084519350435	27.699613985710542	28.181001829123137	22.27853899166197
45	21.878361804775007	26.38758727508867	28.333930987515554	23.40011993262077
46	21.83692779634356	27.82466935661272	27.904674660165796	22.433728186877925
47	21.852748054108297	27.26885096714509	28.929676694198935	21.948724284547676
48	21.655974181339328	26.50058911626533	29.28706385056034	22.556372851835
49	22.013813345065437	26.37071233347295	28.631050495249404	22.984423826212208
50	20.797913534004515	26.59957872913609	29.66720204427864	22.93530569258075
51	20.796406842788826	26.196237490696184	29.14995494993265	23.85740071658234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	216.0
1	390.5
2	565.0
3	5587.0
4	10609.0
5	7650.5
6	4692.0
7	4669.5
8	4647.0
9	4588.0
10	4529.0
11	4492.0
12	4455.0
13	4342.5
14	4230.0
15	4137.0
16	4044.0
17	3814.0
18	3584.0
19	3506.5
20	3429.0
21	3552.5
22	3676.0
23	3923.0
24	4170.0
25	4464.5
26	5669.5
27	6580.0
28	7705.0
29	8830.0
30	10288.0
31	11746.0
32	13825.0
33	15904.0
34	17750.0
35	19596.0
36	21450.5
37	23305.0
38	25072.0
39	26839.0
40	29029.0
41	31219.0
42	33926.5
43	36634.0
44	39952.5
45	43271.0
46	47927.5
47	52584.0
48	54261.5
49	55939.0
50	54925.5
51	53912.0
52	48756.0
53	43600.0
54	40027.0
55	36454.0
56	33404.5
57	30355.0
58	28065.5
59	25776.0
60	24099.0
61	22422.0
62	20509.0
63	18596.0
64	15877.0
65	13158.0
66	11030.5
67	8903.0
68	7629.5
69	6356.0
70	5498.5
71	4641.0
72	4073.5
73	3506.0
74	3014.5
75	2096.0
76	1669.0
77	1320.0
78	971.0
79	706.5
80	442.0
81	317.5
82	193.0
83	154.0
84	115.0
85	79.0
86	43.0
87	25.5
88	8.0
89	8.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	663706.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.04316533754749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.52842768159955	24.35054089899502
2	7.895338679223153	5.375646401054553
3	3.6416325967357435	3.71918101767832
4	2.3851984832055884	3.247988253065414
5	1.811784229203208	3.0839435035362928
6	1.4620703150251726	2.986410088171326
7	1.2096010698946775	2.8825054450429195
8	1.0910086101282133	2.9713109199426126
9	0.9705533252598009	2.973663658865332
>10	7.945520563435624	44.82544272223985
>50	0.0426879572417528	0.9499555879595589
>100	0.013031060621564674	0.7824513412803306
>500	0.0013480407539549662	0.34709327338515544
>1k	0.0013480407539549662	0.7021410863102087
>5k	4.493469179849887E-4	0.8017258024731598
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5241	0.7896568661425389	No Hit
CGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTG	1692	0.2549321536945576	Illumina PCR Primer Index 7 (96% over 26bp)
CGTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1611	0.24272795484747764	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	1287	0.1939111594591581	Illumina PCR Primer Index 7 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	895	0.13484886380415426	Illumina PCR Primer Index 7 (95% over 22bp)
CGTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	813	0.12249399583550548	Illumina PCR Primer Index 7 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0133824313777484E-4	0.0	0.0	0.06267835457265718	0.0
2	3.0133824313777484E-4	0.0	0.0	0.24513866079257984	0.0
3	3.0133824313777484E-4	0.0	0.0	0.6121686409343896	0.0
4	3.0133824313777484E-4	0.0	0.0	0.8660461107779649	0.0
5	3.0133824313777484E-4	0.0	0.0	1.4770093987398034	0.0
6	3.0133824313777484E-4	0.0	0.0	2.3093056262863376	0.0
7	3.0133824313777484E-4	0.0	0.0	2.8660280304833767	0.0
8	3.0133824313777484E-4	0.0	0.0	3.6706011396612355	0.0
9	3.0133824313777484E-4	0.0	0.0	4.154399689018933	0.0
10	4.5200736470666226E-4	0.0	0.0	4.82517861824362	0.0
11	4.5200736470666226E-4	0.0	0.0	5.481493311797694	0.0
12	4.5200736470666226E-4	0.0	0.0	6.0409277601829725	0.0
13	4.5200736470666226E-4	0.0	0.0	6.3516074888580185	0.0
14	4.5200736470666226E-4	0.0	0.0	6.531657089132839	0.0
15	4.5200736470666226E-4	0.0	0.0	6.711556020286091	0.0
16	4.5200736470666226E-4	0.0	0.0	6.989841887823825	0.0
17	4.5200736470666226E-4	0.0	0.0	7.291933476569445	0.0
18	6.026764862755497E-4	0.0	0.0	7.676742413056384	0.0
19	6.026764862755497E-4	0.0	0.0	7.924141110672497	0.0
20	6.026764862755497E-4	0.0	0.0	8.172594492139591	0.0
21	6.026764862755497E-4	0.0	0.0	8.462331212916563	0.0
22	6.026764862755497E-4	0.0	0.0	8.769997559160231	0.0
23	6.026764862755497E-4	0.0	0.0	9.1004149427608	0.0
24	6.026764862755497E-4	0.0	0.0	9.390905009145616	0.0
25	6.026764862755497E-4	0.0	0.0	9.661808089726476	0.0
26	6.026764862755497E-4	0.0	0.0	9.915082883083775	0.0
27	6.026764862755497E-4	0.0	0.0	10.174083103060692	0.0
28	7.533456078444371E-4	0.0	0.0	10.464723838567076	0.0
29	7.533456078444371E-4	0.0	0.0	10.765760743461714	0.0
30	7.533456078444371E-4	0.0	0.0	11.138064142858434	0.0
31	7.533456078444371E-4	0.0	0.0	11.470741563282537	0.0
32	7.533456078444371E-4	0.0	0.0	11.80206296161252	0.0
33	7.533456078444371E-4	0.0	0.0	12.131877668726816	0.0
34	7.533456078444371E-4	0.0	0.0	12.444666765103825	0.0
35	7.533456078444371E-4	0.0	0.0	12.758962552696525	0.0
36	7.533456078444371E-4	0.0	0.0	13.077326406571585	0.0
37	7.533456078444371E-4	0.0	0.0	13.384390076328977	0.0
38	7.533456078444371E-4	0.0	0.0	13.702603261082468	0.0
39	9.040147294133245E-4	0.0	0.0	14.032116629953624	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	40	6.8084773E-9	45.000004	44
TCGGACG	20	7.031129E-4	45.000004	1
CGTAAGC	20	7.031129E-4	45.000004	27
GTATACG	30	2.16411E-6	45.000004	1
TGTAGCG	30	2.16411E-6	45.000004	1
TATAGCG	25	3.889019E-5	45.0	1
CGTTTTT	4980	0.0	44.051205	1
GCGTTAG	50	1.0804797E-9	40.5	1
TTGCACG	50	1.0804797E-9	40.5	1
CGATAGT	50	1.0804797E-9	40.5	10
TATACGG	75	0.0	39.0	2
TACGGGA	255	0.0	38.82353	4
GTCGATG	35	6.2443014E-6	38.571426	1
CGACAGG	70	0.0	38.571426	2
AACACGT	140	0.0	38.571426	41
ACGACCA	160	0.0	37.968754	28
TTAGTCG	30	1.139402E-4	37.500004	1
TAATGCG	30	1.139402E-4	37.500004	1
AGTAACG	30	1.139402E-4	37.500004	1
CACGACC	165	0.0	36.81818	27
>>END_MODULE