FastQCFastQC Report
Sat 18 Jun 2016
SRR3550310_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550310_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959975
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC15150.15781660980754708No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT14750.15364983463111018No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC14680.15292064897523375No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10720.11166957472850857No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCACG302.1651267E-645.0000041
CGAAACG302.1651267E-645.0000042
ACGCTTA207.032703E-445.00000425
CATACCG207.032703E-445.00000416
ACCGGTA253.890324E-545.041
ACCGCGC253.890324E-545.035
ATTCGCG253.890324E-545.01
CGAATAT1700.043.67646814
TTTACGG900.040.02
TTGCGTC451.927765E-840.016
GCGATAT451.927765E-840.09
TTGCACG850.039.7058831
AGTACGG1200.039.3750042
GCGTAAG800.039.3750041
TAGTACG403.457426E-739.3750041
CGCATGG700.038.571432
CACAACG2100.038.5714312
CGGTATT301.1397817E-437.50000443
TACGAAT2050.037.31707412
TAAACGG1100.036.818182
Produced by FastQC (version 0.11.5)