SRR3550309_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688776
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2191	0.3181005145359304	RNA PCR Primer, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	2009	0.29167682962240266	RNA PCR Primer, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2002	0.29066053404880543	Illumina PCR Primer Index 6 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1376	0.1997746727528253	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1054	0.1530250763673531	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	867	0.12587546604411304	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	794	0.11527695506231345	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	719	0.10438807391662892	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	715	0.10380733358885909	Illumina PCR Primer Index 6 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAC	25	3.889171E-5	45.000004	28
AGGCGTA	25	3.889171E-5	45.000004	27
ACGATAG	25	3.889171E-5	45.000004	1
TCGGACG	25	3.889171E-5	45.000004	1
TCGTCAC	25	3.889171E-5	45.000004	45
TAGGCGT	25	3.889171E-5	45.000004	1
TATACGG	25	3.889171E-5	45.000004	2
GACGTAC	20	7.031316E-4	45.0	13
CACGCAA	20	7.031316E-4	45.0	39
TCGATTC	20	7.031316E-4	45.0	44
ATCGTCG	20	7.031316E-4	45.0	38
TCGTCGT	20	7.031316E-4	45.0	28
TCGTCGA	20	7.031316E-4	45.0	39
ACATCGC	20	7.031316E-4	45.0	41
ATACGTT	20	7.031316E-4	45.0	13
GTGCGTA	20	7.031316E-4	45.0	24
GTTGGCC	20	7.031316E-4	45.0	26
GTCGACG	30	2.16423E-6	44.999996	1
AATCGAT	30	2.16423E-6	44.999996	35
GCGAAAG	85	0.0	42.352943	1