##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550309_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	688776
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12600613261786	33.0	31.0	34.0	30.0	34.0
2	32.170560530564366	33.0	31.0	34.0	30.0	34.0
3	32.20601763127635	34.0	31.0	34.0	30.0	34.0
4	35.8215065565583	37.0	35.0	37.0	35.0	37.0
5	33.724058329558524	37.0	35.0	37.0	32.0	37.0
6	34.76945189727865	37.0	35.0	37.0	32.0	37.0
7	35.75971288198195	37.0	35.0	37.0	33.0	37.0
8	35.82808924817357	37.0	35.0	37.0	35.0	37.0
9	37.74447425578127	39.0	38.0	39.0	35.0	39.0
10	37.468695192631564	39.0	37.0	39.0	35.0	39.0
11	37.36641520610474	39.0	37.0	39.0	35.0	39.0
12	37.217111223387576	39.0	37.0	39.0	34.0	39.0
13	37.14345883131816	39.0	37.0	39.0	34.0	39.0
14	38.27022428191459	40.0	37.0	41.0	33.0	41.0
15	38.398106205791144	40.0	37.0	41.0	34.0	41.0
16	38.43467106867835	40.0	37.0	41.0	34.0	41.0
17	38.394213793744264	40.0	37.0	41.0	34.0	41.0
18	38.36268394949882	40.0	37.0	41.0	34.0	41.0
19	38.34066372812061	40.0	37.0	41.0	34.0	41.0
20	38.32528427239044	40.0	37.0	41.0	34.0	41.0
21	38.23666329837277	40.0	37.0	41.0	34.0	41.0
22	38.24188996132269	40.0	37.0	41.0	34.0	41.0
23	38.2388410746019	40.0	37.0	41.0	34.0	41.0
24	38.17396947628837	40.0	37.0	41.0	34.0	41.0
25	38.029260601414684	40.0	36.0	41.0	34.0	41.0
26	38.06822247000476	40.0	36.0	41.0	34.0	41.0
27	38.02886860169344	40.0	36.0	41.0	34.0	41.0
28	37.938946769341555	40.0	36.0	41.0	34.0	41.0
29	37.92148390768552	40.0	36.0	41.0	34.0	41.0
30	37.79055019338653	40.0	36.0	41.0	33.0	41.0
31	37.724048166602785	40.0	36.0	41.0	33.0	41.0
32	37.661847973796995	40.0	36.0	41.0	33.0	41.0
33	37.594259962600326	40.0	36.0	41.0	33.0	41.0
34	37.51184855453732	40.0	35.0	41.0	33.0	41.0
35	37.45229798947698	40.0	35.0	41.0	33.0	41.0
36	37.39710588057656	40.0	35.0	41.0	33.0	41.0
37	37.329876476532284	40.0	35.0	41.0	33.0	41.0
38	37.26070159238998	40.0	35.0	41.0	33.0	41.0
39	37.18648443035181	40.0	35.0	41.0	32.0	41.0
40	37.07013600938476	39.0	35.0	41.0	32.0	41.0
41	37.00683240995621	39.0	35.0	41.0	32.0	41.0
42	36.9595935398446	39.0	35.0	41.0	32.0	41.0
43	36.89596908138495	39.0	35.0	41.0	32.0	41.0
44	36.73402528543387	39.0	35.0	41.0	31.0	41.0
45	36.65436513467368	39.0	35.0	41.0	31.0	41.0
46	36.51744108389375	39.0	35.0	41.0	31.0	41.0
47	36.45339413684565	39.0	35.0	41.0	31.0	41.0
48	36.41479087540797	39.0	35.0	41.0	31.0	41.0
49	36.32114504570426	39.0	35.0	41.0	31.0	41.0
50	36.201972774893434	38.0	35.0	40.0	31.0	41.0
51	35.117716064438945	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	25.0
11	18.0
12	10.0
13	12.0
14	12.0
15	26.0
16	62.0
17	86.0
18	166.0
19	291.0
20	505.0
21	808.0
22	1251.0
23	1812.0
24	2572.0
25	3634.0
26	4443.0
27	5400.0
28	6313.0
29	7490.0
30	9457.0
31	12385.0
32	16074.0
33	23752.0
34	43786.0
35	58773.0
36	48326.0
37	74328.0
38	130455.0
39	236284.0
40	203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82361174024646	22.405252215524353	24.132083580147974	11.63905246408121
2	31.038537928150806	25.43468413533573	25.211534664390168	18.3152432721233
3	33.61150795033508	24.44103743452153	25.48201447204897	16.46544014309442
4	29.894334297362278	26.240606525198324	25.204856150620813	18.66020302681859
5	23.778703090700024	35.057551366481995	22.620126136799193	18.543619406018795
6	27.33254352648757	35.67603981555687	22.858810411512597	14.132606246442966
7	80.07755787077366	7.2553340999105655	7.68813082918104	4.978977200134731
8	79.7870425218068	6.997920949626583	7.891825499146312	5.323211029420305
9	73.1621020476904	10.043468413533573	10.276054914805394	6.518374623970638
10	42.209804058213415	26.461433034832805	17.309401024425938	14.019361882527848
11	32.376418458250576	26.120683647513847	22.93241924805742	18.57047864617815
12	32.06615793813954	23.596060257616408	25.629522515302504	18.70825928894154
13	25.602082534815384	26.251930961589835	26.428766391395754	21.71722011219903
14	19.251251495406343	30.31174140794685	26.91252308442803	23.524484012218778
15	20.093470155754556	28.21497845453384	31.91197138111665	19.77958000859496
16	22.66832758400409	26.633912912180445	30.351812490562967	20.345947013252495
17	21.89637850331603	27.841126868532008	26.42804046598604	23.83445416216593
18	22.749631229891868	28.064711894723395	27.33922204025692	21.84643483512782
19	25.154186557022896	28.305428760583993	25.170882841446275	21.36950184094684
20	26.953465277535805	28.391087958930044	25.73637873561216	18.919068027921995
21	25.615149192190206	28.90126833687585	25.21240577488182	20.271176696052127
22	22.54216174779609	27.89237720245769	25.586257360883653	23.97920368886256
23	24.28118865930288	27.783198020836963	26.4672404381105	21.46837288174965
24	24.321114556837056	25.999454104091896	27.332833896651454	22.346597442419597
25	22.80770526266885	28.1560333112652	25.343217533711975	23.693043892353973
26	22.10370280032986	29.999738666852505	25.33828124092593	22.558277291891706
27	23.597221738271948	28.87106983983182	25.488547800736377	22.043160621159856
28	20.706441571715622	29.765990684925143	26.35356051894956	23.174007224409678
29	22.201847915722965	27.979778621787055	25.806357945108427	24.012015517381556
30	22.365761873236	27.859275003774815	25.545460352857823	24.229502770131365
31	23.635840970068646	29.10423708143141	23.331387853235306	23.928534095264645
32	24.374978222237708	28.130916292089154	24.523357375982904	22.970748109690234
33	23.673589091373685	28.14354739421815	25.456316712545153	22.726546801863016
34	21.555774301079015	27.708572888718535	27.06496742046761	23.67068538973483
35	22.273278976038654	26.614893666445987	26.8177172259196	24.294110131595758
36	22.24482269997793	29.400850203839855	26.04402592424823	22.310301171933983
37	21.799975608906234	29.047179344228024	27.095746657839413	22.05709838902633
38	20.39893956816149	28.35188798680558	26.65815882086484	24.59101362416809
39	23.140759840644854	25.890274922471168	28.050048201447204	22.918917035436774
40	23.416030756007757	26.13433104521644	28.817786914758937	21.631851284016864
41	20.364385518659187	27.79423208706459	27.626978872666875	24.21440352160935
42	21.892458506103583	26.931687515244434	26.836155731326294	24.339698247325693
43	22.81017340906187	26.248882074869044	26.748609126914992	24.192335389154092
44	21.825963738573932	26.71434544757657	25.77746611380187	25.682224700047623
45	21.705460120561693	26.46883747401187	25.710680976108343	26.115021429318098
46	21.466049920438575	28.03436821259742	26.33178275665819	24.16779911030582
47	21.3269626119377	25.81245571855001	29.230693287803295	23.629888381709
48	21.130527196069547	24.23458424799935	29.835679524257525	24.799209031673577
49	21.565211331405276	24.598998803674924	29.751326991648952	24.084462873270844
50	20.202939707539173	25.727958000859495	29.041371940950324	25.027730350651012
51	19.790033334494815	25.18119098226419	27.164274016516256	27.86450166672474
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	582.0
1	914.0
2	1246.0
3	1540.5
4	1835.0
5	1446.5
6	1058.0
7	1090.5
8	1123.0
9	1168.0
10	1213.0
11	1265.5
12	1318.0
13	1308.0
14	1298.0
15	1410.5
16	1523.0
17	1602.0
18	1681.0
19	1650.0
20	1619.0
21	1986.5
22	2354.0
23	2782.5
24	3211.0
25	3688.5
26	5224.5
27	6283.0
28	8153.5
29	10024.0
30	11443.0
31	12862.0
32	13856.5
33	14851.0
34	17684.5
35	20518.0
36	21149.5
37	21781.0
38	23328.0
39	24875.0
40	28757.0
41	32639.0
42	35369.5
43	38100.0
44	41385.5
45	44671.0
46	49071.5
47	53472.0
48	58933.0
49	64394.0
50	62829.5
51	61265.0
52	53874.5
53	46484.0
54	42182.5
55	37881.0
56	36430.0
57	34979.0
58	33337.5
59	31696.0
60	32074.5
61	32453.0
62	29742.0
63	27031.0
64	22781.5
65	18532.0
66	14381.0
67	10230.0
68	8961.5
69	7693.0
70	6343.0
71	4993.0
72	4131.0
73	3269.0
74	2461.5
75	1412.5
76	1171.0
77	806.0
78	441.0
79	276.5
80	112.0
81	115.5
82	119.0
83	89.5
84	60.0
85	34.5
86	9.0
87	5.0
88	1.0
89	2.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	688776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.52415679057823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85880142326519	18.34135314310447
2	11.379048924458381	5.808812577510726
3	4.2421711747348105	3.248335265892082
4	2.0682301015259816	2.1115931756137076
5	1.2155978535616119	1.5513555104298469
6	0.8134063371875839	1.24569065308955
7	0.5622160699748455	1.0045063784154462
8	0.43669492247557	0.8917015736732684
9	0.3273007258920747	0.7518667540805444
>10	5.665820298014271	38.10656831136903
>50	1.3466889755148377	21.36885735580554
>100	0.07475166860646036	3.239117479544433
>500	0.006374173292023752	1.0535266578445204
>1k	0.0028973514963744323	1.2767151636268286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2191	0.3181005145359304	RNA PCR Primer, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	2009	0.29167682962240266	RNA PCR Primer, Index 27 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2002	0.29066053404880543	Illumina PCR Primer Index 6 (95% over 22bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1376	0.1997746727528253	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1054	0.1530250763673531	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	867	0.12587546604411304	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	794	0.11527695506231345	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	719	0.10438807391662892	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	715	0.10380733358885909	Illumina PCR Primer Index 6 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.259254097123013E-4	0.0	0.0	0.31243829634017445	0.0
2	7.259254097123013E-4	0.0	0.0	1.1677236140632077	0.0
3	7.259254097123013E-4	0.0	0.0	1.5929707190726738	0.0
4	7.259254097123013E-4	0.0	0.0	2.0511748376830785	0.0
5	7.259254097123013E-4	0.0	0.0	3.185215512735635	0.0
6	7.259254097123013E-4	0.0	0.0	3.8704890995040477	0.0
7	7.259254097123013E-4	0.0	0.0	4.562150829877928	0.0
8	7.259254097123013E-4	0.0	0.0	5.609225640846952	0.0
9	7.259254097123013E-4	0.0	0.0	5.971172050129505	0.0
10	7.259254097123013E-4	0.0	0.0	6.692741907383533	0.0
11	7.259254097123013E-4	0.0	0.0	7.907795858159982	0.0
12	7.259254097123013E-4	0.0	0.0	8.825801131282159	0.0
13	7.259254097123013E-4	0.0	0.0	9.197184570890972	0.0
14	7.259254097123013E-4	0.0	0.0	9.364002230042859	0.0
15	7.259254097123013E-4	0.0	0.0	9.683990150644041	0.0
16	7.259254097123013E-4	0.0	0.0	10.28055565234561	0.0
17	7.259254097123013E-4	0.0	0.0	10.961328501573806	0.0
18	7.259254097123013E-4	0.0	0.0	11.682898358827833	0.0
19	7.259254097123013E-4	0.0	0.0	12.148506916617304	0.0
20	7.259254097123013E-4	0.0	0.0	12.594805858508426	0.0
21	7.259254097123013E-4	0.0	0.0	13.175255816114383	0.0
22	7.259254097123013E-4	0.0	0.0	13.778064276339478	0.0
23	7.259254097123013E-4	0.0	0.0	14.344866836242842	0.0
24	7.259254097123013E-4	0.0	0.0	14.805103546000442	0.0
25	7.259254097123013E-4	0.0	0.0	15.226140283633576	0.0
26	7.259254097123013E-4	0.0	0.0	15.617414079468507	0.0
27	7.259254097123013E-4	0.0	0.0	15.99475010743696	0.0
28	7.259254097123013E-4	0.0	0.0	16.403591298186928	0.0
29	7.259254097123013E-4	0.0	0.0	16.796897685169053	0.0
30	7.259254097123013E-4	0.0	0.0	17.246245513780966	0.0
31	7.259254097123013E-4	0.0	0.0	17.67599335633065	0.0
32	7.259254097123013E-4	0.0	0.0	18.080043439376517	0.0
33	7.259254097123013E-4	0.0	0.0	18.492369072093105	0.0
34	7.259254097123013E-4	0.0	0.0	18.895983599893142	0.0
35	8.711104916547615E-4	0.0	0.0	19.3088899729375	0.0
36	8.711104916547615E-4	0.0	0.0	19.73616966909416	0.0
37	8.711104916547615E-4	0.0	0.0	20.15560937082593	0.0
38	8.711104916547615E-4	0.0	0.0	20.575774997967407	0.0
39	8.711104916547615E-4	0.0	0.0	20.997392475928315	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAC	25	3.889171E-5	45.000004	28
AGGCGTA	25	3.889171E-5	45.000004	27
ACGATAG	25	3.889171E-5	45.000004	1
TCGGACG	25	3.889171E-5	45.000004	1
TCGTCAC	25	3.889171E-5	45.000004	45
TAGGCGT	25	3.889171E-5	45.000004	1
TATACGG	25	3.889171E-5	45.000004	2
GACGTAC	20	7.031316E-4	45.0	13
CACGCAA	20	7.031316E-4	45.0	39
TCGATTC	20	7.031316E-4	45.0	44
ATCGTCG	20	7.031316E-4	45.0	38
TCGTCGT	20	7.031316E-4	45.0	28
TCGTCGA	20	7.031316E-4	45.0	39
ACATCGC	20	7.031316E-4	45.0	41
ATACGTT	20	7.031316E-4	45.0	13
GTGCGTA	20	7.031316E-4	45.0	24
GTTGGCC	20	7.031316E-4	45.0	26
GTCGACG	30	2.16423E-6	44.999996	1
AATCGAT	30	2.16423E-6	44.999996	35
GCGAAAG	85	0.0	42.352943	1
>>END_MODULE