FastQCFastQC Report
Sat 18 Jun 2016
SRR3550308_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550308_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504219
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC16690.3310069632441459No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC16290.3230739024114522No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT15670.3107776581207769Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6610.1310938302602639No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC5880.11661599424059783No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC5620.11145950469934691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.029516E-445.06
CGGCGCA551.8189894E-1245.022
CGCGGTA207.029516E-445.030
CGCATGT207.029516E-445.035
CGCGCAA207.029516E-445.018
CGATTCC253.887681E-545.037
TAACGCG207.029516E-445.026
ACGGGTA502.1827873E-1145.05
TCTACGG207.029516E-445.02
AACGCGA207.029516E-445.027
TTGCGTC453.8380676E-1045.016
CGTACTG253.887681E-545.011
TCGTATA207.029516E-445.01
TTTGCGC207.029516E-445.013
CGGTCTA253.887681E-545.031
GCTCGAT253.887681E-545.034
TCGCGTG253.887681E-545.01
TCGGTAA207.029516E-445.028
TGCGTCG207.029516E-445.01
TGCGTAG207.029516E-445.01