##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550308_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504219
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17863071403497	33.0	31.0	34.0	30.0	34.0
2	32.2133834702778	33.0	31.0	34.0	30.0	34.0
3	32.25311620545834	34.0	31.0	34.0	30.0	34.0
4	35.85956102407882	37.0	35.0	37.0	35.0	37.0
5	33.69070780752015	37.0	35.0	37.0	32.0	37.0
6	34.78441708860634	37.0	35.0	37.0	32.0	37.0
7	35.7897699214032	37.0	35.0	37.0	33.0	37.0
8	35.86788677142273	37.0	35.0	37.0	35.0	37.0
9	37.80555671246026	39.0	38.0	39.0	35.0	39.0
10	37.533234566725966	39.0	37.0	39.0	35.0	39.0
11	37.42535882225779	39.0	37.0	39.0	35.0	39.0
12	37.28177835424687	39.0	37.0	39.0	35.0	39.0
13	37.21508114529599	39.0	37.0	39.0	34.0	39.0
14	38.35938748837311	40.0	38.0	41.0	34.0	41.0
15	38.514383630922275	40.0	38.0	41.0	34.0	41.0
16	38.54199068262005	40.0	38.0	41.0	35.0	41.0
17	38.50725974229452	40.0	38.0	41.0	34.0	41.0
18	38.466396545945315	40.0	37.0	41.0	34.0	41.0
19	38.44381310501984	40.0	37.0	41.0	34.0	41.0
20	38.4533645102624	40.0	37.0	41.0	34.0	41.0
21	38.3486104252319	40.0	37.0	41.0	34.0	41.0
22	38.370327179261395	40.0	37.0	41.0	34.0	41.0
23	38.35446700739163	40.0	37.0	41.0	34.0	41.0
24	38.29297785287742	40.0	37.0	41.0	34.0	41.0
25	38.158256630551406	40.0	37.0	41.0	34.0	41.0
26	38.17884292341225	40.0	37.0	41.0	34.0	41.0
27	38.15569226863724	40.0	37.0	41.0	34.0	41.0
28	38.10742950979634	40.0	37.0	41.0	34.0	41.0
29	38.06706609627959	40.0	37.0	41.0	34.0	41.0
30	37.91158603701963	40.0	36.0	41.0	34.0	41.0
31	37.87450096089199	40.0	36.0	41.0	34.0	41.0
32	37.81452503773162	40.0	36.0	41.0	34.0	41.0
33	37.74119380665941	40.0	36.0	41.0	33.0	41.0
34	37.646734851324524	40.0	36.0	41.0	33.0	41.0
35	37.59673673542647	40.0	36.0	41.0	33.0	41.0
36	37.52438127083668	40.0	36.0	41.0	33.0	41.0
37	37.44092150434633	40.0	35.0	41.0	33.0	41.0
38	37.37381970929299	40.0	35.0	41.0	33.0	41.0
39	37.3044986404717	40.0	35.0	41.0	33.0	41.0
40	37.18256352894278	40.0	35.0	41.0	32.0	41.0
41	37.152610274503736	39.0	35.0	41.0	32.0	41.0
42	37.09117070161973	39.0	35.0	41.0	32.0	41.0
43	36.99487722596729	39.0	35.0	41.0	32.0	41.0
44	36.85857335800515	39.0	35.0	41.0	31.0	41.0
45	36.80482488759844	39.0	35.0	41.0	31.0	41.0
46	36.7118077660699	39.0	35.0	41.0	31.0	41.0
47	36.641522830357445	39.0	35.0	41.0	31.0	41.0
48	36.601171316431945	39.0	35.0	41.0	31.0	41.0
49	36.50496907097908	39.0	35.0	41.0	31.0	41.0
50	36.39249810102356	39.0	35.0	41.0	31.0	41.0
51	35.30964918021733	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	7.0
10	14.0
11	4.0
12	10.0
13	11.0
14	17.0
15	28.0
16	28.0
17	55.0
18	125.0
19	209.0
20	364.0
21	552.0
22	874.0
23	1159.0
24	1742.0
25	2489.0
26	3186.0
27	3574.0
28	4222.0
29	4995.0
30	6505.0
31	8338.0
32	11358.0
33	16545.0
34	30357.0
35	41156.0
36	35140.0
37	54416.0
38	97593.0
39	178979.0
40	164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62512916014668	21.792713086972128	24.48757385183819	11.094583901043
2	31.24931825258469	25.703117097927684	24.869154077890755	18.178410571596864
3	33.39977271780714	23.95526546996444	25.984939084009127	16.660022728219285
4	29.99133313104028	26.602131216792703	24.738060247630493	18.668475404536522
5	24.480632423609585	34.877503624417166	22.35853865086401	18.28332530110924
6	27.406741911748668	35.413580210186446	22.568764762930392	14.610913115134494
7	79.61699182299755	7.143324626799069	8.178985718507235	5.060697831696148
8	79.917258175515	6.594555143697481	8.108381477096263	5.379805203691253
9	73.37228466202185	9.520069652274112	10.210245944718466	6.8973997409855645
10	41.98929433440628	26.127932505518437	17.690527330386203	14.192245829689085
11	32.66993112119932	26.498803099446867	23.227803791606423	17.603461987747387
12	30.901850188112707	24.570870990581472	26.37921220739401	18.148066613911812
13	25.272946874274872	26.8101757371301	27.40396534045722	20.512912048137814
14	19.34417386096121	30.155150837235407	28.180810322498758	22.319864979304626
15	20.414938746854045	28.760717069368667	31.149560012613563	19.67478417116372
16	22.259573716976156	27.683010755247224	30.474258209230516	19.583157318546107
17	22.280001348620342	27.50947504953205	27.355177016336157	22.855346585511455
18	22.41982154579657	29.004262036932367	28.20976599453809	20.36615042273298
19	24.475674260589148	28.949722243707594	25.684871058012494	20.889732437690768
20	26.84329626610659	28.400357781043557	26.217377766407058	18.538968186442794
21	25.30963728062608	28.612963811359748	26.52220562890331	19.555193279110863
22	23.700812543755788	27.425979584267946	25.704505383573405	23.168702488402857
23	23.998103998460987	27.621529533793847	27.158833760726985	21.22153270701818
24	23.625051812803562	26.080135814001455	28.273230481199636	22.021581891995343
25	22.306577102409868	28.494364551910977	26.069822834918956	23.129235510760203
26	20.992663901994966	30.655330322736745	26.900017651060352	21.451988124207933
27	21.462499429811253	30.903238473758428	26.716565619304312	20.91769647712601
28	19.99666811445027	29.87570876940377	27.76987777136522	22.357745344780742
29	21.485306979705246	28.025718983219594	28.866821758005944	21.622152279069216
30	21.868473817924354	29.45109168833384	26.598759665938807	22.081674827803
31	23.40728929294612	29.10441692994512	25.41633694882581	22.07195682828295
32	24.705336371695633	29.177401089605905	25.284449812482272	20.832812726216186
33	23.986006080691126	30.100016064448187	24.97783701129866	20.936140843562022
34	22.697280348420033	28.135988528794037	27.369654852355822	21.79707627043011
35	23.10127147132496	28.390441455002684	26.23423552067653	22.27405155299582
36	23.370003907032462	30.811809947661633	25.10020447464296	20.717981670662947
37	23.622671894553758	29.44196866837624	26.117222873394297	20.818136563675704
38	22.7666946307061	28.96598501841462	26.212221276865804	22.055099074013473
39	24.406458304823897	26.72945684315744	26.7187472110333	22.145337640985367
40	24.969308970903516	26.29710502777563	28.028693881031852	20.704892120289
41	22.828374178680296	27.65663332797852	26.995611034094313	22.519381459246876
42	23.589948018618895	27.207622084848055	25.921871250389216	23.280558646143838
43	24.00524375321041	27.14038939429097	26.643779786164345	22.210587066334273
44	22.254615553955723	27.747268547992043	26.211427970782537	23.786687927269696
45	22.49439231762389	26.155698218432867	26.648539622663964	24.701369841279288
46	21.972396914832643	28.196478117643327	26.92282520095435	22.908299766569684
47	22.86684952371886	26.986488014136718	28.2075844028091	21.93907805933533
48	22.398600608069113	25.652345508598444	28.10346297937999	23.84559090395245
49	22.471584767729897	25.66999656895119	29.0298461581178	22.828572505201112
50	20.502202416013677	26.69633631418094	28.47016871637126	24.33129255343412
51	20.73027791495362	25.94467880028321	26.79589622763125	26.52914705713192
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	319.0
1	592.5
2	866.0
3	1077.0
4	1288.0
5	1039.5
6	791.0
7	823.5
8	856.0
9	864.5
10	873.0
11	904.5
12	936.0
13	987.0
14	1038.0
15	1106.5
16	1175.0
17	1188.0
18	1201.0
19	1337.5
20	1474.0
21	1518.0
22	1562.0
23	1762.5
24	1963.0
25	2748.5
26	4038.5
27	4543.0
28	5887.0
29	7231.0
30	8337.0
31	9443.0
32	10596.0
33	11749.0
34	13022.0
35	14295.0
36	15068.5
37	15842.0
38	18504.0
39	21166.0
40	23601.0
41	26036.0
42	28262.0
43	30488.0
44	32952.0
45	35416.0
46	38937.5
47	42459.0
48	45230.0
49	48001.0
50	46989.0
51	45977.0
52	40722.5
53	35468.0
54	32061.0
55	28654.0
56	25767.0
57	22880.0
58	22446.5
59	22013.0
60	21969.5
61	21926.0
62	19158.5
63	16391.0
64	13168.0
65	9945.0
66	7825.0
67	5705.0
68	5014.5
69	4324.0
70	3388.0
71	2452.0
72	2115.5
73	1779.0
74	1491.5
75	880.5
76	557.0
77	425.0
78	293.0
79	177.5
80	62.0
81	41.5
82	21.0
83	17.0
84	13.0
85	9.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.103233116596368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65340072749387	21.144488580857463
2	10.644826484437948	6.19597733324633
3	3.7429182571597783	3.2679306772345678
4	1.7710779573819075	2.061763786454039
5	1.103637512918329	1.6059709907341377
6	0.7653461227399103	1.3364427974989672
7	0.610212783042763	1.2431415412934406
8	0.44479485900084137	1.035597477645208
9	0.43634718521170973	1.1429202465888335
>10	7.49733506693283	52.10440158679403
>50	0.27703846254568487	4.841840840661006
>100	0.048929678708317485	2.680551875872348
>500	0.002067451213027499	0.36322330319527285
>1k	0.002067451213027499	0.9757489619243526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1669	0.3310069632441459	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1629	0.3230739024114522	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	1567	0.3107776581207769	Illumina Single End Adapter 2 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	661	0.1310938302602639	No Hit
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	588	0.11661599424059783	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCC	562	0.11145950469934691	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.949795624520298E-4	0.0	0.0	0.3335852080147714	0.0
2	5.949795624520298E-4	0.0	0.0	1.2516386728782534	0.0
3	5.949795624520298E-4	0.0	0.0	1.6766524069898199	0.0
4	5.949795624520298E-4	0.0	0.0	2.125465323599468	0.0
5	5.949795624520298E-4	0.0	0.0	3.2565214718207764	0.0
6	5.949795624520298E-4	0.0	0.0	3.9484827029524867	0.0
7	5.949795624520298E-4	0.0	0.0	4.6456004236254484	0.0
8	5.949795624520298E-4	0.0	0.0	5.712597105622756	0.0
9	5.949795624520298E-4	0.0	0.0	6.078906189572388	0.0
10	5.949795624520298E-4	0.0	0.0	6.891053292319409	0.0
11	5.949795624520298E-4	0.0	0.0	8.179779024590506	0.0
12	5.949795624520298E-4	0.0	0.0	9.08573457168413	0.0
13	5.949795624520298E-4	0.0	0.0	9.46195998167463	0.0
14	5.949795624520298E-4	0.0	0.0	9.636883973035527	0.0
15	5.949795624520298E-4	0.0	0.0	9.950438202447746	0.0
16	5.949795624520298E-4	0.0	0.0	10.567035355668866	0.0
17	7.933060832693731E-4	0.0	0.0	11.270499525007983	0.0
18	7.933060832693731E-4	0.0	0.0	12.060235730902644	0.0
19	7.933060832693731E-4	0.0	0.0	12.557440318591723	0.0
20	7.933060832693731E-4	0.0	0.0	13.063569599717583	0.0
21	7.933060832693731E-4	0.0	0.0	13.67064707993947	0.0
22	7.933060832693731E-4	0.0	0.0	14.312233374783576	0.0
23	9.916326040867163E-4	0.0	0.0	14.924665671067531	0.0
24	9.916326040867163E-4	0.0	0.0	15.393707892800549	0.0
25	9.916326040867163E-4	0.0	0.0	15.833001136410964	0.0
26	0.0011899591249040595	0.0	0.0	16.243338707982048	0.0
27	0.0011899591249040595	0.0	0.0	16.624323954472164	0.0
28	0.0011899591249040595	0.0	0.0	17.0112589965868	0.0
29	0.0011899591249040595	0.0	0.0	17.428537996386492	0.0
30	0.0011899591249040595	0.0	0.0	17.91761119672206	0.0
31	0.0011899591249040595	0.0	0.0	18.32437889091843	0.0
32	0.0011899591249040595	0.0	0.0	18.760697236716585	0.0
33	0.0011899591249040595	0.0	0.0	19.167464930912956	0.0
34	0.0011899591249040595	0.0	0.0	19.57383597206769	0.0
35	0.0011899591249040595	0.0	0.0	19.980008686701613	0.0
36	0.0011899591249040595	0.0	0.0	20.431796501123518	0.0
37	0.0011899591249040595	0.0	0.0	20.87664288731682	0.0
38	0.0011899591249040595	0.0	0.0	21.31950600830195	0.0
39	0.0013882856457214027	0.0	0.0	21.77783859791083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.029516E-4	45.0	6
CGGCGCA	55	1.8189894E-12	45.0	22
CGCGGTA	20	7.029516E-4	45.0	30
CGCATGT	20	7.029516E-4	45.0	35
CGCGCAA	20	7.029516E-4	45.0	18
CGATTCC	25	3.887681E-5	45.0	37
TAACGCG	20	7.029516E-4	45.0	26
ACGGGTA	50	2.1827873E-11	45.0	5
TCTACGG	20	7.029516E-4	45.0	2
AACGCGA	20	7.029516E-4	45.0	27
TTGCGTC	45	3.8380676E-10	45.0	16
CGTACTG	25	3.887681E-5	45.0	11
TCGTATA	20	7.029516E-4	45.0	1
TTTGCGC	20	7.029516E-4	45.0	13
CGGTCTA	25	3.887681E-5	45.0	31
GCTCGAT	25	3.887681E-5	45.0	34
TCGCGTG	25	3.887681E-5	45.0	1
TCGGTAA	20	7.029516E-4	45.0	28
TGCGTCG	20	7.029516E-4	45.0	1
TGCGTAG	20	7.029516E-4	45.0	1
>>END_MODULE