##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550307_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	788243
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29355795103794	33.0	31.0	34.0	30.0	34.0
2	32.333715618153285	34.0	31.0	34.0	30.0	34.0
3	32.361803403265235	34.0	31.0	34.0	30.0	34.0
4	35.946262764147605	37.0	35.0	37.0	35.0	37.0
5	33.929898521141325	37.0	35.0	37.0	32.0	37.0
6	34.94389547385768	37.0	35.0	37.0	32.0	37.0
7	35.89784749119244	37.0	35.0	37.0	33.0	37.0
8	35.98580513877066	37.0	35.0	37.0	35.0	37.0
9	37.90967252484323	39.0	38.0	39.0	35.0	39.0
10	37.62343972607432	39.0	37.0	39.0	35.0	39.0
11	37.48724822167783	39.0	37.0	39.0	35.0	39.0
12	37.26995609222029	39.0	37.0	39.0	35.0	39.0
13	37.17558037305755	39.0	37.0	39.0	34.0	39.0
14	38.32553920554956	40.0	37.0	41.0	34.0	41.0
15	38.46554044882099	40.0	37.0	41.0	34.0	41.0
16	38.50834323933102	40.0	37.0	41.0	35.0	41.0
17	38.4302087046761	40.0	37.0	41.0	34.0	41.0
18	38.40833981399137	40.0	37.0	41.0	34.0	41.0
19	38.36885706565108	40.0	37.0	41.0	34.0	41.0
20	38.36595440746064	40.0	37.0	41.0	34.0	41.0
21	38.26708895607065	40.0	37.0	41.0	34.0	41.0
22	38.27829489129621	40.0	36.0	41.0	34.0	41.0
23	38.286099591115935	40.0	36.0	41.0	34.0	41.0
24	38.23819811910794	40.0	36.0	41.0	34.0	41.0
25	38.08422910193938	40.0	36.0	41.0	34.0	41.0
26	38.11430992726862	40.0	36.0	41.0	34.0	41.0
27	38.093756620737516	40.0	36.0	41.0	34.0	41.0
28	38.00517353151249	40.0	36.0	41.0	34.0	41.0
29	37.974759052728665	40.0	36.0	41.0	34.0	41.0
30	37.81943892936569	40.0	36.0	41.0	34.0	41.0
31	37.75721065711969	40.0	36.0	41.0	34.0	41.0
32	37.69681176997449	40.0	35.0	41.0	34.0	41.0
33	37.60901904615709	40.0	35.0	41.0	33.0	41.0
34	37.504034923240674	40.0	35.0	41.0	33.0	41.0
35	37.45096245700881	40.0	35.0	41.0	33.0	41.0
36	37.379471812626306	40.0	35.0	41.0	33.0	41.0
37	37.30587648732688	40.0	35.0	41.0	33.0	41.0
38	37.20710491561612	39.0	35.0	41.0	33.0	41.0
39	37.14747990150246	39.0	35.0	41.0	33.0	41.0
40	37.067362475784755	39.0	35.0	41.0	32.0	41.0
41	37.01531634280292	39.0	35.0	41.0	32.0	41.0
42	36.95000780216253	39.0	35.0	41.0	32.0	41.0
43	36.871649986108345	39.0	35.0	41.0	32.0	41.0
44	36.70418513072745	39.0	35.0	41.0	32.0	41.0
45	36.6455674201991	39.0	35.0	41.0	32.0	41.0
46	36.54884217176683	39.0	35.0	41.0	31.0	41.0
47	36.459988861302925	38.0	35.0	41.0	31.0	41.0
48	36.41975253824011	38.0	35.0	41.0	31.0	41.0
49	36.340789578848145	38.0	35.0	40.0	31.0	41.0
50	36.22762270010644	38.0	35.0	40.0	31.0	41.0
51	35.143355538837646	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	13.0
10	21.0
11	16.0
12	11.0
13	12.0
14	25.0
15	35.0
16	41.0
17	94.0
18	159.0
19	277.0
20	502.0
21	863.0
22	1258.0
23	1908.0
24	2757.0
25	3744.0
26	4756.0
27	5600.0
28	6512.0
29	7725.0
30	9595.0
31	12564.0
32	16977.0
33	26639.0
34	53743.0
35	73860.0
36	54332.0
37	85007.0
38	150214.0
39	268725.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0526588881855	20.67141731674116	23.655903065425257	11.620020729648092
2	32.16939446338248	24.47062644387581	25.204156586230386	18.155822506511317
3	32.70159075310532	23.993743046243353	25.911552655716573	17.393113544934746
4	29.07377547279202	25.70755465002544	26.419390974610625	18.799278902571924
5	24.4949844147046	34.21318045323587	22.97375301778766	18.31808211427187
6	28.257529721164666	33.708386880695414	23.261481548202774	14.77260184993714
7	81.91826632142626	5.945374713127805	7.863311187032425	4.27304777841351
8	83.14453791533829	5.507311831503737	7.049856452895871	4.298293800262102
9	76.91447941814897	7.209832500891222	9.761203080775852	6.1144850001839535
10	46.0356768153983	23.57572474478048	16.636620940496776	13.751977499324447
11	33.687454249514424	24.34388887690725	22.8498064683099	19.118850405268425
12	29.89090927543917	23.332906223080954	26.8110214743423	19.96516302713757
13	25.060292321022832	25.946313509920166	28.94145587084186	20.051938298215145
14	21.610848431257875	27.8122355669508	30.016885655819337	20.56003034597199
15	22.003240117577956	25.549989026226683	32.04494045617912	20.401830400016237
16	24.957278402726065	25.33203593308155	29.595188285845865	20.115497378346525
17	24.443858048850416	25.331401610924548	28.258290907753064	21.966449432471965
18	26.075715229948127	25.515989358611495	28.287596591406455	20.120698820033926
19	26.015581489464545	26.902617593813076	25.519287833827892	21.562513082894487
20	28.258037178890266	26.562240324366975	26.960087181237256	18.219635315505496
21	26.090050910696323	27.750071995564817	26.75177578487852	19.40810130886034
22	25.426169341180323	24.977449847318656	27.659237062682447	21.937143748818574
23	25.27164846373517	26.031058950095336	26.92760988679887	21.769682699370627
24	25.246529306317978	24.20712901985809	28.96670189269045	21.579639781133483
25	22.992909546929056	26.94955743343106	27.162435949320198	22.895097070319686
26	22.132261244311717	27.889749734536178	27.98616670240015	21.991822318751957
27	22.55104073236299	27.306427078959157	27.727743855638426	22.414788333039432
28	20.73269283710734	26.838551055956096	29.96715479871055	22.46160130822602
29	23.234840017608782	25.36958780477594	29.144438961081796	22.251133216533482
30	24.350232098477246	26.29024298344546	28.840344919016093	20.519179999061205
31	26.032581323272137	26.73033569597193	26.907945899931875	20.32913708082406
32	27.974241445848552	26.36166765832364	25.23257421886398	20.431516676963827
33	26.09246133489292	25.73889016458123	26.53179286083099	21.636855639694865
34	23.1748331415566	26.10349854042472	29.222333721961373	21.499334596057306
35	24.322829381294856	27.155585270024602	27.515626526337687	21.00595882234286
36	25.56597394458308	28.94817968570606	26.4958902267448	18.989956142966065
37	24.664475295054952	27.866533543589984	27.34676996814434	20.122221193210724
38	24.00275042087275	28.30180540772325	26.534076420596186	21.16136775080781
39	24.676273687175147	27.05574296251283	27.21178621313478	21.05619713717724
40	25.553794959168684	25.660361081544654	27.231577064433175	21.55426689485349
41	22.957387506137067	26.000230893265147	26.51631540020019	24.526066200397594
42	24.10677925462072	25.072598170868627	26.829163088032498	23.991459486478153
43	24.647094867953157	24.790197946572313	26.412667159746423	24.150040025728106
44	23.14502000017761	25.56813063991688	27.461074820835705	23.825774539069805
45	23.030842011917645	26.356339352204838	27.07667559369382	23.536143042183692
46	24.323717432314655	26.399980716606425	27.204554940544984	22.071746910533935
47	22.14875362039371	25.791919496906413	29.74374653501522	22.31558034768466
48	23.70335036276884	24.10918967881732	29.671180080254437	22.516279878159402
49	23.63941068934326	24.321560736980853	29.801089258008002	22.237939315667884
50	22.172223540202705	25.37377433101214	29.23603508055257	23.217967048232588
51	21.97710604470956	25.131336402606806	27.896981007125977	24.99457654555765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	291.0
1	760.5
2	1230.0
3	1584.0
4	1938.0
5	1587.0
6	1236.0
7	1239.5
8	1243.0
9	1358.5
10	1474.0
11	1424.0
12	1374.0
13	1501.5
14	1629.0
15	1707.5
16	1786.0
17	1773.0
18	1760.0
19	1832.0
20	1904.0
21	1940.5
22	1977.0
23	2540.5
24	3104.0
25	3562.5
26	4919.0
27	5817.0
28	7638.5
29	9460.0
30	10407.5
31	11355.0
32	13843.5
33	16332.0
34	18924.5
35	21517.0
36	23141.0
37	24765.0
38	26257.5
39	27750.0
40	31653.0
41	35556.0
42	39685.5
43	43815.0
44	49966.5
45	56118.0
46	59965.5
47	63813.0
48	66411.5
49	69010.0
50	69890.5
51	70771.0
52	63023.5
53	55276.0
54	49636.0
55	43996.0
56	42666.0
57	41336.0
58	40181.0
59	39026.0
60	37741.0
61	36456.0
62	33326.0
63	30196.0
64	26097.0
65	21998.0
66	17694.5
67	13391.0
68	11655.5
69	9920.0
70	7936.5
71	5953.0
72	4990.0
73	4027.0
74	3436.0
75	2205.0
76	1565.0
77	1118.5
78	672.0
79	459.0
80	246.0
81	185.5
82	125.0
83	95.5
84	66.0
85	63.5
86	61.0
87	35.0
88	9.0
89	15.0
90	21.0
91	13.0
92	5.0
93	5.0
94	5.0
95	3.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	788243.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.37145583973884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.26479338836606	16.889334274440486
2	10.761067585830327	5.030036317413571
3	3.8077734817264606	2.669796293276957
4	1.9559739035246635	1.828558308396331
5	1.1597467905793244	1.3552485450651766
6	0.7618498089014726	1.0683323499152546
7	0.5212487340660881	0.8527639238821746
8	0.4100535862278048	0.7666839425959746
9	0.34602616621401516	0.7278421736758488
>10	6.095677101322969	37.19848900070552
>50	1.8438505078562724	27.8705599224401
>100	0.06751193336088301	2.414113818874881
>500	0.0016601295088741724	0.2595696150477813
>1k	0.0027668825147902872	1.0686715142699637
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	2456	0.3115790435183059	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1978	0.2509378453091242	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	1335	0.16936401591894887	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	1256	0.15934172583835188	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	1238	0.15705816607315257	Illumina Single End Adapter 2 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	907	0.11506603927976525	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2686443139996168E-4	0.0	0.0	0.16467003195715027	0.0
2	1.2686443139996168E-4	0.0	0.0	0.8877972909369319	0.0
3	2.5372886279992335E-4	0.0	0.0	1.1647423446830483	0.0
4	2.5372886279992335E-4	0.0	0.0	1.4550081637261605	0.0
5	2.5372886279992335E-4	0.0	0.0	2.347499438624891	0.0
6	2.5372886279992335E-4	0.0	0.0	2.7969801190749553	0.0
7	2.5372886279992335E-4	0.0	0.0	3.2502667324670185	0.0
8	2.5372886279992335E-4	0.0	0.0	3.900700672254622	0.0
9	2.5372886279992335E-4	0.0	0.0	4.112056814966958	0.0
10	2.5372886279992335E-4	0.0	0.0	4.788624827622954	0.0
11	2.5372886279992335E-4	0.0	0.0	5.609183969917906	0.0
12	2.5372886279992335E-4	0.0	0.0	6.4231461617800605	0.0
13	2.5372886279992335E-4	0.0	0.0	6.68803909454318	0.0
14	2.5372886279992335E-4	0.0	0.0	6.802217082803145	0.0
15	2.5372886279992335E-4	0.0	0.0	7.067871202154666	0.0
16	2.5372886279992335E-4	0.0	0.0	7.542471040021923	0.0
17	3.805932941998851E-4	0.0	0.0	8.097376062965354	0.0
18	3.805932941998851E-4	0.0	0.0	8.670676428461782	0.0
19	3.805932941998851E-4	0.0	0.0	9.069030743057661	0.0
20	3.805932941998851E-4	0.0	0.0	9.446071833178348	0.0
21	3.805932941998851E-4	0.0	0.0	9.884388443665214	0.0
22	3.805932941998851E-4	0.0	0.0	10.340212345685277	0.0
23	3.805932941998851E-4	0.0	0.0	10.789819890566742	0.0
24	3.805932941998851E-4	0.0	0.0	11.185002594377622	0.0
25	5.074577255998467E-4	0.0	0.0	11.546819952730313	0.0
26	5.074577255998467E-4	0.0	0.0	11.850913994796022	0.0
27	5.074577255998467E-4	0.0	0.0	12.172769057257725	0.0
28	5.074577255998467E-4	0.0	0.0	12.492340559954227	0.0
29	5.074577255998467E-4	0.0	0.0	12.837031220067923	0.0
30	5.074577255998467E-4	0.0	0.0	13.230057228545004	0.0
31	5.074577255998467E-4	0.0	0.0	13.5736061087761	0.0
32	6.343221569998084E-4	0.0	0.0	13.914744564810597	0.0
33	6.343221569998084E-4	0.0	0.0	14.264509802180292	0.0
34	6.343221569998084E-4	0.0	0.0	14.616431734883786	0.0
35	6.343221569998084E-4	0.0	0.0	14.984084857080875	0.0
36	6.343221569998084E-4	0.0	0.0	15.321036786879173	0.0
37	6.343221569998084E-4	0.0	0.0	15.66179464961947	0.0
38	6.343221569998084E-4	0.0	0.0	16.037820824288957	0.0
39	6.343221569998084E-4	0.0	0.0	16.470555399794225	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	20	7.0319354E-4	45.000004	1
ACGCACT	30	2.1646301E-6	45.000004	40
GCGAACT	20	7.0319354E-4	45.000004	13
ATTACGT	20	7.0319354E-4	45.000004	34
CCCCGAC	20	7.0319354E-4	45.000004	1
GTTAACG	40	6.8102963E-9	45.000004	1
CTACGTA	20	7.0319354E-4	45.000004	35
CGAATCC	20	7.0319354E-4	45.000004	13
CGAATAC	20	7.0319354E-4	45.000004	29
GTATACG	20	7.0319354E-4	45.000004	1
CACGTAC	20	7.0319354E-4	45.000004	20
CGTTTAG	20	7.0319354E-4	45.000004	30
TATCGCG	20	7.0319354E-4	45.000004	32
TCGAATC	20	7.0319354E-4	45.000004	12
ATCGCGA	20	7.0319354E-4	45.000004	22
TACGTCC	20	7.0319354E-4	45.000004	36
TCTTACG	35	1.2112832E-7	45.0	1
TATTCGC	25	3.8896862E-5	45.0	16
CTATTCG	25	3.8896862E-5	45.0	15
TATAGCG	45	3.8380676E-10	45.0	1
>>END_MODULE