SRR3550305_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231402
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1465	0.6330973803165054	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	807	0.3487437446521638	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	572	0.24718887477204174	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	343	0.14822689518673132	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	318	0.1374231856250162	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	315	0.1361267404776104	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	274	0.11840865679639762	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCT	20	7.0215977E-4	45.000004	14
AACGTAG	20	7.0215977E-4	45.000004	1
TTGGCCC	20	7.0215977E-4	45.000004	18
CGTATAG	20	7.0215977E-4	45.000004	2
TTAGCCG	20	7.0215977E-4	45.000004	1
ACGCACG	20	7.0215977E-4	45.000004	2
CGGCATA	20	7.0215977E-4	45.000004	6
GAATCGC	20	7.0215977E-4	45.000004	17
CCGTGAT	20	7.0215977E-4	45.000004	4
TAACGCG	20	7.0215977E-4	45.000004	34
TGCGGAA	20	7.0215977E-4	45.000004	21
AACGGGC	20	7.0215977E-4	45.000004	4
CTAACGC	20	7.0215977E-4	45.000004	33
TACGCAC	20	7.0215977E-4	45.000004	1
ATAAGGA	20	7.0215977E-4	45.000004	23
GGACGTT	20	7.0215977E-4	45.000004	9
CCCCAAA	20	7.0215977E-4	45.000004	14
AACCTAT	20	7.0215977E-4	45.000004	31
CGGCTAA	20	7.0215977E-4	45.000004	30
GGACCGT	20	7.0215977E-4	45.000004	8