##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550305_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	231402
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26639354888895	33.0	31.0	34.0	30.0	34.0
2	32.38078754721221	34.0	31.0	34.0	30.0	34.0
3	32.35487160871557	34.0	31.0	34.0	30.0	34.0
4	35.91919257396219	37.0	35.0	37.0	35.0	37.0
5	35.96669000267932	37.0	35.0	37.0	35.0	37.0
6	35.965588024304026	37.0	35.0	37.0	35.0	37.0
7	36.14700823674817	37.0	36.0	37.0	35.0	37.0
8	36.08951089446072	37.0	36.0	37.0	35.0	37.0
9	37.92625820001556	39.0	38.0	39.0	35.0	39.0
10	37.623533936612475	39.0	37.0	39.0	35.0	39.0
11	37.440367844703154	39.0	37.0	39.0	35.0	39.0
12	37.08448500877261	39.0	35.0	39.0	34.0	39.0
13	36.91335857079887	39.0	35.0	39.0	33.0	39.0
14	38.01282616399167	40.0	36.0	41.0	33.0	41.0
15	38.20325234872646	40.0	36.0	41.0	34.0	41.0
16	38.228563279487645	40.0	36.0	41.0	34.0	41.0
17	38.18028798368208	40.0	36.0	41.0	34.0	41.0
18	38.13904374205927	40.0	36.0	41.0	34.0	41.0
19	38.056749725585775	40.0	36.0	41.0	34.0	41.0
20	38.03088132341121	40.0	35.0	41.0	34.0	41.0
21	37.79303549666814	40.0	35.0	41.0	34.0	41.0
22	37.857836146619306	40.0	35.0	41.0	34.0	41.0
23	37.85586987147907	40.0	35.0	41.0	34.0	41.0
24	37.81585293126248	40.0	35.0	41.0	34.0	41.0
25	37.56761393592104	39.0	35.0	41.0	33.0	41.0
26	37.5376012307586	39.0	35.0	41.0	33.0	41.0
27	37.55579467766052	39.0	35.0	41.0	33.0	41.0
28	37.55303757098037	39.0	35.0	41.0	33.0	41.0
29	37.57202617090604	39.0	35.0	41.0	34.0	41.0
30	37.35152678023526	39.0	35.0	41.0	33.0	41.0
31	37.31173887866138	39.0	35.0	41.0	33.0	41.0
32	37.173775507558275	39.0	35.0	41.0	33.0	41.0
33	37.09556529329911	39.0	35.0	41.0	33.0	41.0
34	37.00669397844444	39.0	35.0	41.0	33.0	41.0
35	36.8702820200344	39.0	35.0	41.0	32.0	41.0
36	36.74038685923198	39.0	35.0	41.0	32.0	41.0
37	36.666174017510656	39.0	35.0	41.0	32.0	41.0
38	36.607920415553885	39.0	35.0	41.0	32.0	41.0
39	36.569070275969956	39.0	35.0	41.0	32.0	41.0
40	36.418708567773834	38.0	35.0	41.0	31.0	41.0
41	36.294107224656656	38.0	35.0	41.0	31.0	41.0
42	36.26195970648482	38.0	35.0	41.0	31.0	41.0
43	36.24249142185461	38.0	35.0	41.0	31.0	41.0
44	36.118395692344926	38.0	35.0	40.0	31.0	41.0
45	36.00636554567376	38.0	35.0	40.0	31.0	41.0
46	35.91796959403981	38.0	35.0	40.0	31.0	41.0
47	35.810286860096284	37.0	35.0	40.0	30.0	41.0
48	35.74032635845844	37.0	35.0	40.0	30.0	41.0
49	35.62328761203447	37.0	35.0	40.0	30.0	41.0
50	35.56661999464136	37.0	35.0	40.0	30.0	41.0
51	34.535405916975655	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	7.0
11	6.0
12	4.0
13	9.0
14	8.0
15	18.0
16	16.0
17	43.0
18	60.0
19	117.0
20	205.0
21	331.0
22	475.0
23	713.0
24	1033.0
25	1386.0
26	1818.0
27	2069.0
28	2204.0
29	2512.0
30	3154.0
31	4059.0
32	5309.0
33	7844.0
34	18113.0
35	27949.0
36	15918.0
37	24060.0
38	42236.0
39	69666.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70664903501266	21.137241683304378	25.883095219574592	12.273014062108366
2	32.41069653676286	24.519234924503678	25.043863060820566	18.026205477912896
3	30.6414810589364	24.51188840200171	26.935808679268114	17.910821859793778
4	28.247811168442798	25.84463401353489	26.763813623045607	19.14374119497671
5	24.72580185132367	29.64926837278848	25.331241735162184	20.293688040725662
6	27.50019446677211	31.952619251346142	24.281985462528414	16.265200819353336
7	78.10304146031581	7.355597617998116	8.870709846933043	5.670651074753027
8	79.23527022238356	6.104528050751505	8.927753433418898	5.732448293446037
9	74.77333817339522	7.238917554731593	10.950640011754437	7.037104260118754
10	47.168563798065705	20.99117553002999	18.237958185322512	13.602302486581793
11	36.577903388907615	23.023569372779836	21.376651887191986	19.02187535112056
12	31.08011166714203	22.205944633149237	27.092678542104213	19.621265157604515
13	25.057691809059556	28.164838679008824	27.95827175218883	18.819197759742785
14	19.46525959153335	30.978988945644375	29.36793977580142	20.187811687020858
15	18.35550254535397	25.58620928081866	36.86182487618949	19.196463297637877
16	22.00067415147665	23.708092410610107	32.7378328622916	21.553400575621644
17	21.800589450393687	24.01059627833813	27.301406210836554	26.88740806043163
18	23.648888082211908	24.278528275468663	30.232236540738626	21.840347101580797
19	25.10695672466098	26.092687185071867	26.458716865022776	22.341639225244382
20	27.29535613348199	25.733571879240458	26.650590746838837	20.320481240438717
21	24.340325494161675	27.124657522406892	27.538223524429352	20.996793459002085
22	23.546468915566848	24.86841081753831	26.786285338933975	24.798834927960865
23	22.129886517834763	26.23529615128651	27.007113162375436	24.627704168503296
24	23.793225641956422	23.421145884650954	29.94701860830935	22.838609865083274
25	22.401707850407515	25.002808964486047	28.166135124156234	24.429348060950208
26	21.46264941530324	27.329063707314543	28.02957623529615	23.178710642086067
27	21.420731022203785	27.762076386548085	28.487221372330403	22.329971218917727
28	18.900441655646883	26.979887814279913	30.65963129100008	23.460039239073126
29	21.813986050250215	23.266436763727192	31.548560513737993	23.371016672284593
30	24.730123335148356	25.251294284405496	28.71539571827383	21.303186662172322
31	26.864504196160794	25.557687487575738	24.990708809776923	22.587099506486545
32	27.116878851522458	24.680858419546936	26.668308830520047	21.53395389841056
33	26.955687504861668	25.444032462986492	25.81265503323221	21.78762499891963
34	24.028314362019344	24.31093940415381	28.534325546019478	23.126420687807364
35	22.537402442502657	25.32130232236541	29.781505777823874	22.35978945730806
36	27.2897382045099	25.943163844737725	26.678248243316826	20.088849707435543
37	24.216731056775654	25.755179298363885	28.826890000950726	21.20119964390973
38	21.966102280879163	28.09094130560669	27.35672120379253	22.58623520972161
39	25.672638957312383	25.464775585344984	27.356289055410066	21.506296401932566
40	27.62681394283541	22.686493634454326	28.241328942705767	21.445363480004495
41	22.963068599234234	23.395216981702838	27.08662846474966	26.555085954313274
42	22.630314344733407	23.792793493573956	28.23960034917589	25.337291812516742
43	23.84292270594031	22.939732586580927	28.780650124026586	24.436694583452173
44	22.28891712258321	23.38527756890606	29.019196031149256	25.306609277361474
45	23.69901729457827	24.23272054692699	27.14496849638292	24.923293662111824
46	25.329080993249843	24.402122712854684	28.06371595751117	22.2050803363843
47	22.637660867235372	23.834711886673407	30.137596044978004	23.390031201113214
48	22.320031806120948	21.986845403237655	30.522640253757533	25.170482536883863
49	23.272486841081754	21.597479710633444	31.715801937753348	23.414231510531454
50	22.26514896154744	23.566347741160403	29.618153689250743	24.550349608041415
51	21.358069506745835	23.469978651869905	26.646701411396617	28.525250429987643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	271.0
1	343.5
2	416.0
3	637.5
4	859.0
5	679.0
6	499.0
7	525.0
8	551.0
9	579.0
10	607.0
11	655.0
12	703.0
13	743.5
14	784.0
15	736.0
16	688.0
17	661.5
18	635.0
19	670.5
20	706.0
21	620.5
22	535.0
23	619.5
24	704.0
25	936.5
26	1384.5
27	1600.0
28	1667.0
29	1734.0
30	1998.5
31	2263.0
32	2886.0
33	3509.0
34	3987.5
35	4466.0
36	4727.0
37	4988.0
38	6200.0
39	7412.0
40	7772.5
41	8133.0
42	11105.5
43	14078.0
44	16094.0
45	18110.0
46	19131.0
47	20152.0
48	21967.0
49	23782.0
50	23322.0
51	22862.0
52	19858.5
53	16855.0
54	15244.0
55	13633.0
56	12410.5
57	11188.0
58	10760.5
59	10333.0
60	9532.0
61	8731.0
62	8256.0
63	7781.0
64	6830.5
65	5880.0
66	5212.0
67	4544.0
68	4056.5
69	3569.0
70	2976.0
71	2383.0
72	1951.0
73	1519.0
74	1376.5
75	976.5
76	719.0
77	569.0
78	419.0
79	312.5
80	206.0
81	164.0
82	122.0
83	78.0
84	34.0
85	21.5
86	9.0
87	6.5
88	4.0
89	2.5
90	1.0
91	1.5
92	2.0
93	5.5
94	9.0
95	10.0
96	11.0
97	5.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	231402.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.777158365096234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.122285654014	24.62295053629614
2	9.701108811159308	6.3594955964079825
3	3.293472385196514	3.2385199782197214
4	1.5913615568183315	2.086412390558422
5	0.9822405632391525	1.6097527246955516
6	0.7066858280485715	1.3897891980190318
7	0.5458356955449787	1.2523660123940155
8	0.4865057286379158	1.27570202504732
9	0.3981700001318444	1.1745793035496668
>10	7.035215631468615	51.73248286531663
>50	0.09888327817843816	2.1123412935065384
>100	0.034279536435191896	1.9165780762482605
>500	0.0026368874180916846	0.5959326194242055
>1k	0.0013184437090458423	0.6330973803165054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	1465	0.6330973803165054	TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	807	0.3487437446521638	TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGCT	572	0.24718887477204174	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTG	343	0.14822689518673132	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	318	0.1374231856250162	No Hit
TCTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	315	0.1361267404776104	TruSeq Adapter, Index 21 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCGTCTGGTATCGTATGCCGTCTTCTGC	274	0.11840865679639762	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2618819197759743	0.0
2	0.0	0.0	0.0	1.568698628361034	0.0
3	0.0	0.0	0.0	2.1516667963111815	0.0
4	0.0	0.0	0.0	2.7514887511776043	0.0
5	0.0	0.0	0.0	4.444646113689596	0.0
6	0.0	0.0	0.0	5.360800684523038	0.0
7	0.0	0.0	0.0	6.279980294033759	0.0
8	0.0	0.0	0.0	7.565621731877858	0.0
9	0.0	0.0	0.0	8.025427610824453	0.0
10	0.0	0.0	0.0	9.06344802551404	0.0
11	0.0	0.0	0.0	10.788152219946241	0.0
12	0.0	0.0	0.0	12.041382529105194	0.0
13	0.0	0.0	0.0	12.532303091589528	0.0
14	0.0	0.0	0.0	12.70170525751722	0.0
15	0.0	0.0	0.0	13.077674350264907	0.0
16	0.0	0.0	0.0	13.832637574437559	0.0
17	0.0	0.0	0.0	14.734531248649537	0.0
18	0.0	0.0	0.0	15.79891271466971	0.0
19	0.0	0.0	0.0	16.42768861116153	0.0
20	0.0	0.0	0.0	16.993802992195402	0.0
21	0.0	0.0	0.0	17.62171459192228	0.0
22	0.0	0.0	0.0	18.327845048875982	0.0
23	0.0	0.0	0.0	18.979092661256168	0.0
24	0.0	0.0	0.0	19.532674739198452	0.0
25	0.0	0.0	0.0	20.001987882559355	0.0
26	0.0	0.0	0.0	20.463954503418293	0.0
27	0.0	0.0	0.0	20.906906595448614	0.0
28	0.0	0.0	0.0	21.33905497791722	0.0
29	0.0	0.0	0.0	21.793242927891722	0.0
30	0.0	0.0	0.0	22.297992238615052	0.0
31	0.0	0.0	0.0	22.77551620124286	0.0
32	0.0	0.0	0.0	23.217171848125773	0.0
33	0.0	0.0	0.0	23.641541559709943	0.0
34	0.0	0.0	0.0	24.090543729094822	0.0
35	0.0	0.0	0.0	24.558560427308322	0.0
36	0.0	0.0	0.0	24.994598145219143	0.0
37	0.0	0.0	0.0	25.441871721074147	0.0
38	0.0	0.0	0.0	25.90297404516815	0.0
39	0.0	0.0	0.0	26.363212072497213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGCT	20	7.0215977E-4	45.000004	14
AACGTAG	20	7.0215977E-4	45.000004	1
TTGGCCC	20	7.0215977E-4	45.000004	18
CGTATAG	20	7.0215977E-4	45.000004	2
TTAGCCG	20	7.0215977E-4	45.000004	1
ACGCACG	20	7.0215977E-4	45.000004	2
CGGCATA	20	7.0215977E-4	45.000004	6
GAATCGC	20	7.0215977E-4	45.000004	17
CCGTGAT	20	7.0215977E-4	45.000004	4
TAACGCG	20	7.0215977E-4	45.000004	34
TGCGGAA	20	7.0215977E-4	45.000004	21
AACGGGC	20	7.0215977E-4	45.000004	4
CTAACGC	20	7.0215977E-4	45.000004	33
TACGCAC	20	7.0215977E-4	45.000004	1
ATAAGGA	20	7.0215977E-4	45.000004	23
GGACGTT	20	7.0215977E-4	45.000004	9
CCCCAAA	20	7.0215977E-4	45.000004	14
AACCTAT	20	7.0215977E-4	45.000004	31
CGGCTAA	20	7.0215977E-4	45.000004	30
GGACCGT	20	7.0215977E-4	45.000004	8
>>END_MODULE