FastQCFastQC Report
Sat 18 Jun 2016
SRR3550302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193234
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61823.199229949180786No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC5160.26703375182421313No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC3850.19924029932620554No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT3630.18785513936470807No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC3030.15680470310607864No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC2670.13817444135090098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.11902667232474616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAT351.2049168E-745.0000047
ACGGGAA351.2049168E-745.0000045
CGTAAGG351.2049168E-745.0000042
TCGTTGA207.018709E-445.024
GATACCG207.018709E-445.027
GATCGTT253.8787257E-545.09
CTCGTAG207.018709E-445.015
CCCTAGC207.018709E-445.021
GGCGTGA253.8787257E-545.035
CGTTGAT207.018709E-445.025
TGAACGG207.018709E-445.02
TACGGGA406.7648216E-945.04
CGACAGG207.018709E-445.02
CCTAACA207.018709E-445.027
TCGATCA207.018709E-445.017
ATCGTTG207.018709E-445.023
AAACGCT207.018709E-445.010
AGCGGGT253.8787257E-545.04
CGCTCGT207.018709E-445.013
AGGCCCA207.018709E-445.01