##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550302_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193234
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.69576782553795	28.0	16.0	31.0	16.0	33.0
2	27.117500025875362	30.0	25.0	33.0	16.0	33.0
3	27.27816015815022	31.0	25.0	33.0	16.0	34.0
4	29.04240972085658	32.0	19.0	35.0	19.0	37.0
5	32.056278915718764	33.0	32.0	35.0	28.0	37.0
6	31.986653487481497	35.0	32.0	35.0	23.0	37.0
7	32.14174006644793	35.0	32.0	35.0	27.0	37.0
8	33.4918440854094	35.0	33.0	37.0	30.0	37.0
9	36.04947369510542	37.0	35.0	39.0	31.0	39.0
10	35.30626080296428	37.0	34.0	39.0	30.0	39.0
11	34.876652141962595	37.0	34.0	39.0	27.0	39.0
12	34.58362917498991	37.0	33.0	39.0	26.0	39.0
13	34.99903226140327	37.0	34.0	39.0	27.0	39.0
14	35.8453015514868	38.0	34.0	40.0	27.0	41.0
15	36.39390583437697	38.0	34.0	40.0	30.0	41.0
16	36.14836933458915	38.0	34.0	40.0	29.0	41.0
17	35.85302793504249	38.0	34.0	40.0	29.0	41.0
18	35.3547822846911	38.0	34.0	39.0	27.0	40.0
19	34.58582858089156	37.0	32.0	39.0	26.0	40.0
20	35.25588147013465	35.0	34.0	39.0	30.0	40.0
21	35.56928904851113	36.0	34.0	39.0	31.0	40.0
22	36.00298084188083	37.0	34.0	39.0	31.0	40.0
23	36.56338429054928	38.0	35.0	40.0	33.0	41.0
24	36.556765372553485	38.0	35.0	40.0	33.0	41.0
25	35.01483175838621	35.0	34.0	39.0	27.0	41.0
26	35.333000403655674	36.0	34.0	39.0	30.0	41.0
27	35.8987393522879	37.0	35.0	40.0	31.0	41.0
28	36.05542502872165	37.0	35.0	40.0	31.0	41.0
29	36.08491776809464	37.0	35.0	40.0	31.0	41.0
30	35.52848360019458	37.0	35.0	40.0	30.0	41.0
31	35.106404669985615	36.0	34.0	40.0	29.0	41.0
32	34.705212333233284	37.0	34.0	40.0	25.0	41.0
33	34.37259488495813	37.0	34.0	40.0	21.0	41.0
34	33.94383493588085	37.0	34.0	40.0	18.0	41.0
35	33.65527288158398	37.0	33.0	40.0	15.0	41.0
36	33.39963463986669	37.0	33.0	40.0	12.0	41.0
37	33.2934007472805	37.0	33.0	40.0	10.0	41.0
38	33.028173095831995	37.0	33.0	40.0	10.0	41.0
39	32.93318463624414	37.0	33.0	40.0	10.0	41.0
40	32.7287330386992	37.0	33.0	40.0	10.0	41.0
41	32.48260658062246	36.0	32.0	40.0	10.0	41.0
42	32.59791237566888	37.0	32.0	40.0	10.0	41.0
43	32.44567208669282	36.0	32.0	40.0	10.0	41.0
44	32.298891499425565	36.0	32.0	40.0	10.0	41.0
45	32.25375451525094	36.0	32.0	40.0	10.0	41.0
46	32.1212830040262	36.0	31.0	40.0	10.0	41.0
47	32.18414978730451	36.0	31.0	40.0	10.0	41.0
48	32.08131591748864	36.0	31.0	40.0	10.0	41.0
49	32.06213192295352	36.0	31.0	40.0	9.0	41.0
50	32.01298943250153	35.0	31.0	40.0	8.0	41.0
51	30.883493588084914	35.0	28.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	7.0
11	7.0
12	7.0
13	13.0
14	13.0
15	25.0
16	70.0
17	132.0
18	250.0
19	438.0
20	714.0
21	1080.0
22	1613.0
23	2476.0
24	3905.0
25	5682.0
26	6951.0
27	6501.0
28	6009.0
29	5830.0
30	6494.0
31	8042.0
32	10798.0
33	13957.0
34	15911.0
35	18790.0
36	24233.0
37	29016.0
38	20507.0
39	3757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.33281927611083	19.092913255431238	19.81328337663144	28.76098409182649
2	46.41315710485732	17.7639545835619	20.666652866472774	15.156235445108004
3	26.818779303849222	16.251798337766648	41.31467547119037	15.614746887193764
4	24.398397797489054	22.56021197097819	37.57413291656748	15.467257314965273
5	20.082387157539564	24.77773062711531	39.00814556444518	16.131736650899946
6	21.328544666052558	26.915035656250968	39.331587608805904	12.424832068890568
7	64.90162186778724	5.486612086899821	25.510520922818962	4.101245122493971
8	64.91662957864558	4.273057536458387	25.842760590786302	4.9675522941097325
9	59.90819421012865	6.4791910326340085	28.31023525880539	5.302379498431953
10	35.372139478559674	19.42722295248248	33.86929836364201	11.331339205315835
11	27.071322852086073	20.788267075152405	37.33452705010505	14.805883022656468
12	24.568140182369564	19.57833507560781	41.973462227144296	13.880062514878333
13	19.05772276100479	23.55434343852531	41.92378152913048	15.464152271339412
14	16.008052413136404	25.766687021952663	41.90152871647847	16.32373184843247
15	15.112247327074947	21.78136352815757	47.69502261506774	15.411366529699741
16	16.496061769667865	20.489665379798584	47.1863129676972	15.827959882836353
17	16.963370835360237	20.352525952989637	41.64536261734477	21.03874059430535
18	19.27248827846031	20.533653497831644	43.71125164308559	16.48260658062246
19	20.164670813624934	22.046327250897875	40.80130825838103	16.987693677096164
20	21.425836032996266	22.312325988180135	40.562737406460556	15.699100572363042
21	18.742043325708728	22.883654015338916	42.00451266340292	16.36978999554944
22	18.269041680035606	21.408758293054017	40.68797416603703	19.634225860873343
23	17.196766614570937	22.555036898268423	41.25360961321506	18.99458687394558
24	17.457072771872443	20.4394671745138	43.67761367047207	18.425846383141682
25	16.080503431073208	22.54106420195204	42.10439156670151	19.274040800273244
26	17.287847894262914	24.47395385905172	40.96691058509372	17.271287661591643
27	17.549706573377357	23.45756957885258	42.551517848825775	16.441205998944287
28	15.7068631814277	22.326298684496518	44.00674829481354	17.960089839262242
29	17.37789415941294	20.60662202303942	43.664675988697645	18.350807828849998
30	19.266795698479562	22.410134862394816	40.64812610617179	17.674943332953827
31	19.796723143960175	24.130329031122887	38.5273813097074	17.545566515209536
32	20.411004274610058	24.293343821480693	37.080948487326246	18.214703416583003
33	19.046855108314272	23.99525963339785	37.448896156991005	19.508989101296873
34	17.713756378277115	25.008021362700145	37.20049266692197	20.077729592100766
35	19.26938323483445	24.989908608215945	35.17289917923347	20.567808977716137
36	21.316124491549107	24.991978637299855	35.362824347682086	18.329072523468955
37	18.617324073403232	27.20846227889502	36.17375824130329	18.000455406398462
38	18.127244687787865	27.074427895711935	34.400260823664574	20.39806659283563
39	18.784478921928855	25.826717865386005	34.71387022987673	20.674932982808407
40	20.37788380926752	26.2257159713094	34.05870602481965	19.337694194603433
41	18.713062918534003	26.535185319353737	34.11304428827225	20.638707473840007
42	20.839500294979143	24.991978637299855	34.09027396834926	20.078247099371747
43	20.57298405042591	24.970243331918812	34.49134210335655	19.965430514298728
44	19.36408706542327	25.610399826117558	33.237421985778894	21.78809112268027
45	19.053065195565996	26.45962925779107	32.07717068424812	22.410134862394816
46	20.309572849498537	26.27436165478125	33.241562043946715	20.1745034517735
47	19.261103118498816	25.886748708819358	34.63883167558504	20.213316497096784
48	19.051512673753066	24.356479708539904	35.159961497459044	21.43204612024799
49	19.266278191208585	23.014583354896136	36.15564548681909	21.563492967076186
50	17.934214475713382	24.176387178239857	35.481851020006836	22.407547326039932
51	17.5486715588354	24.558307544220998	33.2783050601861	24.614715836757505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	294.0
1	375.5
2	457.0
3	5780.0
4	11103.0
5	7604.5
6	4106.0
7	3914.0
8	3722.0
9	3723.5
10	3725.0
11	3579.0
12	3433.0
13	3235.0
14	3037.0
15	2818.5
16	2600.0
17	2434.0
18	2268.0
19	2099.0
20	1930.0
21	1763.5
22	1597.0
23	1607.0
24	1617.0
25	1572.0
26	1693.0
27	1859.0
28	2036.5
29	2214.0
30	2292.5
31	2371.0
32	2623.0
33	2875.0
34	3541.5
35	4208.0
36	4799.5
37	5391.0
38	5687.0
39	5983.0
40	6669.5
41	7356.0
42	8872.0
43	10388.0
44	11414.5
45	12441.0
46	13448.0
47	14455.0
48	15530.5
49	16606.0
50	16217.0
51	15828.0
52	13739.5
53	11651.0
54	10163.0
55	8675.0
56	7591.5
57	6508.0
58	5997.5
59	5487.0
60	5317.0
61	5147.0
62	4543.0
63	3939.0
64	3393.5
65	2848.0
66	2248.0
67	1648.0
68	1508.0
69	1368.0
70	1155.5
71	943.0
72	879.0
73	815.0
74	593.0
75	275.5
76	180.0
77	140.5
78	101.0
79	97.5
80	94.0
81	68.0
82	42.0
83	27.0
84	12.0
85	10.0
86	8.0
87	6.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	193234.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.06574412370494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.99295361127422	35.24949025533809
2	7.854374633000587	6.922177256590454
3	2.6553141514973575	3.510251819038057
4	1.3623018203170876	2.40123373733401
5	0.894891368173811	1.971702702422969
6	0.7868467410452143	2.080379229328172
7	0.6376981796829124	1.9670451369841746
8	0.5143863769817968	1.8133454775039588
9	0.4732824427480916	1.8769988718341493
>10	4.770405167351733	36.267427057350154
>50	0.042278332354668234	1.2932506701719158
>100	0.012918379330593071	1.1804340850988957
>500	0.0011743981209630064	0.26703375182421313
>1k	0.0	0.0
>5k	0.0011743981209630064	3.199229949180786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6182	3.199229949180786	No Hit
CCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	516	0.26703375182421313	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	385	0.19924029932620554	No Hit
CTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGCT	363	0.18785513936470807	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCC	303	0.15680470310607864	No Hit
TCTGTCTCTTATACACATCTGACGCGGTCCAATTCGTATGCCGTCTTCTGC	267	0.13817444135090098	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	230	0.11902667232474616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20027531386815986	0.0
2	0.0	0.0	0.0	0.9713611476241241	0.0
3	0.0	0.0	0.0	1.2658227848101267	0.0
4	0.0	0.0	0.0	1.7424469813800885	0.0
5	0.0	0.0	0.0	2.922881066478984	0.0
6	0.0	0.0	0.0	3.584255358787791	0.0
7	0.0	0.0	0.0	4.288065247316725	0.0
8	0.0	0.0	0.0	5.312212136580519	0.0
9	0.0	0.0	0.0	5.663082066303032	0.0
10	0.0	0.0	0.0	6.444518045478539	0.0
11	0.0	0.0	0.0	7.906476085989008	0.0
12	0.0	0.0	0.0	8.861277000941863	0.0
13	0.0	0.0	0.0	9.249407454174731	0.0
14	0.0	0.0	0.0	9.41345725907449	0.0
15	0.0	0.0	0.0	9.70636637444756	0.0
16	0.0	0.0	0.0	10.319094983284515	0.0
17	0.0	0.0	0.0	11.05757785896892	0.0
18	5.175072709771572E-4	0.0	0.0	11.902667232474617	0.0
19	5.175072709771572E-4	0.0	0.0	12.314085512901457	0.0
20	5.175072709771572E-4	0.0	0.0	12.748791620522269	0.0
21	5.175072709771572E-4	0.0	0.0	13.29993686411294	0.0
22	5.175072709771572E-4	0.0	0.0	13.916805531117712	0.0
23	5.175072709771572E-4	0.0	0.0	14.488133558276493	0.0
24	5.175072709771572E-4	0.0	0.0	14.932154796774896	0.0
25	5.175072709771572E-4	0.0	0.0	15.340468033575872	0.0
26	5.175072709771572E-4	0.0	0.0	15.706345674156722	0.0
27	5.175072709771572E-4	0.0	0.0	16.06342569113096	0.0
28	5.175072709771572E-4	0.0	0.0	16.40032292453709	0.0
29	5.175072709771572E-4	0.0	0.0	16.78586584141507	0.0
30	5.175072709771572E-4	0.0	0.0	17.215914383597088	0.0010350145419543144
31	5.175072709771572E-4	0.0	0.0	17.61077243135266	0.0010350145419543144
32	5.175072709771572E-4	0.0	0.0	17.992175290062825	0.0010350145419543144
33	5.175072709771572E-4	0.0	0.0	18.35391287247586	0.0010350145419543144
34	5.175072709771572E-4	0.0	0.0	18.737903267540908	0.0010350145419543144
35	5.175072709771572E-4	0.0	0.0	19.160706707929247	0.0010350145419543144
36	5.175072709771572E-4	0.0	0.0	19.531759421219867	0.0010350145419543144
37	5.175072709771572E-4	0.0	0.0	19.911609758117102	0.0010350145419543144
38	5.175072709771572E-4	0.0	0.0	20.253682064233004	0.0010350145419543144
39	5.175072709771572E-4	0.0	0.0	20.621629733897763	0.0010350145419543144
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTAAT	35	1.2049168E-7	45.000004	7
ACGGGAA	35	1.2049168E-7	45.000004	5
CGTAAGG	35	1.2049168E-7	45.000004	2
TCGTTGA	20	7.018709E-4	45.0	24
GATACCG	20	7.018709E-4	45.0	27
GATCGTT	25	3.8787257E-5	45.0	9
CTCGTAG	20	7.018709E-4	45.0	15
CCCTAGC	20	7.018709E-4	45.0	21
GGCGTGA	25	3.8787257E-5	45.0	35
CGTTGAT	20	7.018709E-4	45.0	25
TGAACGG	20	7.018709E-4	45.0	2
TACGGGA	40	6.7648216E-9	45.0	4
CGACAGG	20	7.018709E-4	45.0	2
CCTAACA	20	7.018709E-4	45.0	27
TCGATCA	20	7.018709E-4	45.0	17
ATCGTTG	20	7.018709E-4	45.0	23
AAACGCT	20	7.018709E-4	45.0	10
AGCGGGT	25	3.8787257E-5	45.0	4
CGCTCGT	20	7.018709E-4	45.0	13
AGGCCCA	20	7.018709E-4	45.0	1
>>END_MODULE