FastQCFastQC Report
Sat 18 Jun 2016
SRR3550300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746942
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC514506.888085018649373No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG230103.0805604718974164No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT164642.2041872059677994No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC155762.085302473284405No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130611.748596276551593No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT123631.6551485925279341No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC88091.1793419033874117No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT57900.775160588104565No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC54950.7356662230802392No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA23310.3120724232939104No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT17820.23857274058762262No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15790.2113952622827475No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGT14940.20001552998760277No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG10750.14392014373271284No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCG10620.14217971408757307No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT10320.13816333798340433No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTAT10200.13655678754173683No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT9700.1298628273681223No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC9500.12718524329867648No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC8010.10723724198130512No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT7880.10549681233616533No Hit
GACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG7870.10536293313269303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGCG302.1644773E-645.0000041
TAGCACG302.1644773E-645.0000041
CGAACCC351.2111559E-745.034
ACGTTAG253.8894897E-545.01
CGTTCAT253.8894897E-545.017
TCGACGG406.8102963E-945.02
TACGGCT17700.043.7288177
CGTTTTT61750.043.4331971
ACGGCTG18100.042.762438
CGGCTGT18700.041.991989
GAATCTG57500.041.9478231
TAGCCGT2000.041.62544
TGATACC27700.041.344774
AATCTGT58900.041.2563672
TCACGAC603.6379788E-1241.25000425
GATACCT28150.040.523985
CGATGAA6900.040.43478419
GAATGAC7700.040.3246731
CATCGTT900.040.036
TAACGGG1300.039.807693
Produced by FastQC (version 0.11.5)