##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550300_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	746942
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.248647150648914	28.0	16.0	31.0	16.0	33.0
2	26.432773896768424	30.0	25.0	31.0	16.0	33.0
3	26.53819707554268	30.0	25.0	31.0	16.0	33.0
4	30.1893815048558	33.0	28.0	35.0	19.0	37.0
5	32.00347550412214	33.0	32.0	35.0	26.0	37.0
6	31.86265466394981	35.0	32.0	35.0	25.0	37.0
7	32.706385502488814	35.0	32.0	35.0	28.0	37.0
8	33.21025327267713	35.0	32.0	35.0	28.0	37.0
9	35.150892572649546	37.0	34.0	39.0	28.0	39.0
10	34.40260287947391	35.0	32.0	39.0	28.0	39.0
11	34.75091104797963	37.0	34.0	39.0	28.0	39.0
12	34.55475257784406	37.0	33.0	39.0	27.0	39.0
13	34.638297217186874	37.0	33.0	39.0	27.0	39.0
14	35.93011505578746	38.0	34.0	39.0	30.0	41.0
15	35.790393363875644	38.0	34.0	40.0	27.0	41.0
16	35.705017257029326	37.0	34.0	40.0	28.0	41.0
17	35.089262620123115	37.0	33.0	39.0	27.0	41.0
18	34.62167477528376	37.0	32.0	39.0	27.0	40.0
19	34.162186622254474	36.0	32.0	39.0	26.0	40.0
20	34.11081315550605	35.0	32.0	39.0	26.0	40.0
21	35.431884938857365	37.0	34.0	39.0	30.0	40.0
22	36.01287248541386	38.0	34.0	39.0	30.0	40.0
23	36.539230622993486	38.0	35.0	40.0	31.0	41.0
24	36.49234077076935	38.0	35.0	40.0	31.0	41.0
25	35.39874046445373	37.0	34.0	40.0	28.0	41.0
26	35.225757823231255	37.0	33.0	39.0	27.0	41.0
27	36.02337530892626	38.0	35.0	40.0	30.0	41.0
28	35.71704228708521	38.0	34.0	40.0	29.0	41.0
29	36.0455698032779	38.0	35.0	40.0	30.0	41.0
30	35.53717557722018	38.0	34.0	40.0	28.0	41.0
31	35.40808255527203	37.0	34.0	40.0	28.0	41.0
32	35.43047786842887	38.0	34.0	40.0	28.0	41.0
33	34.999030714566864	38.0	34.0	40.0	25.0	41.0
34	35.30976836220215	38.0	35.0	40.0	26.0	41.0
35	35.14025051476554	38.0	35.0	40.0	25.0	41.0
36	35.0663478556568	38.0	35.0	40.0	24.0	41.0
37	35.2403533339938	38.0	35.0	40.0	25.0	41.0
38	34.924446610312444	38.0	35.0	40.0	24.0	41.0
39	34.722643792958486	38.0	34.0	40.0	23.0	41.0
40	34.24346602547453	38.0	33.0	40.0	22.0	41.0
41	33.70277344157913	37.0	33.0	40.0	19.0	41.0
42	34.21572223813897	38.0	33.0	40.0	21.0	41.0
43	34.303944616851105	38.0	34.0	40.0	21.0	41.0
44	34.50452377828533	38.0	34.0	40.0	22.0	41.0
45	34.52977875122834	38.0	34.0	40.0	22.0	41.0
46	34.26046065156331	38.0	34.0	40.0	21.0	41.0
47	34.09380915787303	37.0	33.0	40.0	20.0	41.0
48	34.15008260346854	37.0	34.0	40.0	20.0	41.0
49	34.2333782274929	37.0	34.0	40.0	20.0	41.0
50	34.15864551732263	37.0	34.0	40.0	20.0	41.0
51	32.25298617563345	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	20.0
10	29.0
11	23.0
12	27.0
13	30.0
14	47.0
15	81.0
16	171.0
17	398.0
18	797.0
19	1467.0
20	2469.0
21	3868.0
22	5187.0
23	7045.0
24	9820.0
25	12989.0
26	15425.0
27	16299.0
28	17425.0
29	20509.0
30	25840.0
31	33936.0
32	44469.0
33	56799.0
34	71280.0
35	90611.0
36	108941.0
37	112491.0
38	75092.0
39	13347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.01298628273682	13.853150579295315	14.958725041569492	21.17513809639838
2	30.506384699213594	37.76919760838191	17.519566445587476	14.204851246817022
3	23.381333490418264	36.282602933025586	27.576304452019034	12.759759124537112
4	21.655496678456963	18.16981773685239	44.28550007898873	15.889185505701917
5	28.59914156654734	21.470475619258256	28.34744866401943	21.582934150174978
6	19.095190791252868	34.64767010022197	32.244002881080455	14.0131362274447
7	62.137756345204856	4.9183202979615555	28.512655601104235	4.43126775572936
8	57.719742630619244	12.72053251791973	21.85698487968276	7.702739971778263
9	52.753627457018084	6.189369455727486	20.772831089964146	20.284171997290283
10	31.21139258469868	22.44899871743723	28.988462290244758	17.351146407619332
11	23.9614320790637	20.078265782349902	34.227557159725926	21.732744978860474
12	20.971240069510085	17.14001890374353	40.079283264296286	21.809457762450098
13	19.40900364419192	20.682462627620353	44.65246297570628	15.25607075248145
14	16.315189131150746	30.28588565109473	33.486134130896374	19.91279108685815
15	14.195211944167015	24.194649651512435	44.62528549740141	16.984852906919144
16	16.715487949532896	26.81172032098878	31.431891632817543	25.040900096660785
17	15.84848622784634	25.68352027332778	33.812129991351405	24.655863507474475
18	16.275560886922946	27.19930061504106	33.14500992044898	23.380128577587016
19	16.861684039724636	27.967767242972013	28.851905502703023	26.31864321460033
20	18.9293144581507	27.847811476660837	35.68014651740028	17.542727547788182
21	18.722203330379067	28.736769387716848	34.8427320996811	17.698295182222985
22	16.740523360582213	19.926580644815793	34.519146064888574	28.813749929713417
23	19.32613241724257	25.75286970072643	35.391636833917495	19.529361048113508
24	24.391719839023647	23.03284592377989	30.452699138621202	22.12273509857526
25	18.502507557481035	33.33056649646157	27.165696934969514	21.001229011087876
26	17.922275089632127	22.861212784928416	32.786749171957126	26.42976295348233
27	24.721865954786317	22.48099584706711	28.70691432534253	24.090223872804046
28	18.22698415673506	22.30695288255313	35.35455229455567	24.111510666156143
29	19.96821707709568	19.269501514173793	39.742443188360006	21.019838220370524
30	18.752192271956858	23.051053495452123	40.46552476631385	17.731229466277167
31	26.891110688647846	19.120226202302188	31.28649881784663	22.702164291203335
32	22.90070714995274	21.709717755863238	35.461789536536976	19.927785557647045
33	18.153216715621827	18.778164837430484	29.204409445445563	33.864209001502125
34	19.197742261112644	19.82108383247963	30.81176316233389	30.169410744073836
35	16.61307035887659	18.59863282557414	33.091725997467	31.696570818082257
36	21.357749329934585	20.98984927879273	36.30147990071519	21.3509214905575
37	16.973071537013585	20.1758637216812	41.60001713653804	21.25104760476717
38	21.84466799296331	21.67651571340211	27.041323154943758	29.437493138690822
39	24.031183144072767	19.17940081023694	32.02082624889215	24.768589796798146
40	19.685865836972617	20.7319979329051	34.422619159185054	25.159517070937238
41	16.920055372438554	26.261209036310717	33.28785903055391	23.530876560696814
42	19.989102232837354	21.795400446085505	36.32356996928811	21.89192735178903
43	25.498900851739492	19.35116782829189	30.469701797462186	24.680229522506433
44	22.031429481807155	19.832463564774773	29.79387957833406	28.34222737508401
45	20.47267391577927	18.416021592037936	28.91563200355583	32.19567248862696
46	28.0373844287776	22.28164971309687	27.96923991421021	21.711725943915326
47	18.474392924751857	19.37754203137593	39.71968372376972	22.428381320102496
48	20.244008236248597	21.517868857287446	29.936059292421636	28.30206361404232
49	17.81892034455152	19.03159816960353	39.477897882298755	23.671583603546193
50	19.488929528664876	18.40383858452196	36.659874528410505	25.447357358402662
51	19.310066912825896	18.42499149867058	31.31889758508693	30.946044003416596
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1851.0
1	1838.5
2	1826.0
3	11613.5
4	21401.0
5	14636.5
6	7872.0
7	7463.5
8	7055.0
9	6909.5
10	6764.0
11	6606.5
12	6449.0
13	6079.0
14	5709.0
15	5311.5
16	4914.0
17	4535.0
18	4156.0
19	3834.0
20	3512.0
21	3462.0
22	3412.0
23	3470.5
24	3529.0
25	3560.0
26	3993.0
27	4395.0
28	5107.0
29	5819.0
30	6812.0
31	7805.0
32	8720.0
33	9635.0
34	11328.0
35	13021.0
36	13332.0
37	13643.0
38	15968.5
39	18294.0
40	20369.5
41	22445.0
42	24967.5
43	27490.0
44	32877.5
45	38265.0
46	109782.5
47	181300.0
48	121895.0
49	62490.0
50	60012.5
51	57535.0
52	47031.0
53	36527.0
54	32863.0
55	29199.0
56	27726.5
57	26254.0
58	25133.5
59	24013.0
60	22270.5
61	20528.0
62	18976.0
63	17424.0
64	15462.0
65	13500.0
66	11515.5
67	9531.0
68	8349.0
69	7167.0
70	6346.5
71	5526.0
72	5066.0
73	4606.0
74	3987.5
75	2752.5
76	2136.0
77	1789.5
78	1443.0
79	1094.0
80	745.0
81	606.5
82	468.0
83	330.0
84	192.0
85	129.5
86	67.0
87	52.0
88	37.0
89	28.0
90	19.0
91	13.5
92	8.0
93	4.5
94	1.0
95	1.5
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	746942.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.73358031224331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22751006949395	24.62465742549873
2	9.052963091064965	5.92671788810299
3	3.8957027209640143	3.825608936679011
4	2.2058156460419216	2.8881697441486445
5	1.5414211892335543	2.5228117146385074
6	1.1755747764461022	2.3088462814707578
7	0.9447750101230933	2.1648108069605336
8	0.777686865167801	2.0365180366997557
9	0.6440792967547568	1.8974719248997522
>10	4.422073439550513	24.692893089918698
>50	0.06267540624321362	1.4123148613051024
>100	0.0384409158006825	2.3446943302071968
>500	0.004178360413117663	1.0067838164064151
>1k	0.003342688330494131	1.555789418777744
>5k	0.001253508123935299	2.748310556564394
>10k+	0.002507016247870598	18.043601167721764
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	51450	6.888085018649373	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCG	23010	3.0805604718974164	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCT	16464	2.2041872059677994	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGC	15576	2.085302473284405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13061	1.748596276551593	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	12363	1.6551485925279341	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	8809	1.1793419033874117	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	5790	0.775160588104565	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTC	5495	0.7356662230802392	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTA	2331	0.3120724232939104	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCCCTT	1782	0.23857274058762262	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1579	0.2113952622827475	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGT	1494	0.20001552998760277	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	1075	0.14392014373271284	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTTGTCCCTTCG	1062	0.14217971408757307	No Hit
CTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGCT	1032	0.13816333798340433	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTAT	1020	0.13655678754173683	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTT	970	0.1298628273681223	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTGC	950	0.12718524329867648	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTC	801	0.10723724198130512	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCT	788	0.10549681233616533	No Hit
GACTGTCTCTTATACACATCTGACGCTTGTCCCTTCGTATGCCGTCTTCTG	787	0.10536293313269303	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3387920347229101E-4	0.0	0.0	0.14699936541257555	0.0
2	1.3387920347229101E-4	0.0	0.0	1.630782577495977	0.0
3	1.3387920347229101E-4	0.0	0.0	2.012070548985062	0.0
4	1.3387920347229101E-4	0.0	0.0	4.642930776419052	0.0
5	1.3387920347229101E-4	0.0	0.0	12.700852275009305	0.0
6	1.3387920347229101E-4	0.0	0.0	15.214300440998096	0.0
7	1.3387920347229101E-4	0.0	0.0	16.624048453561322	0.0
8	1.3387920347229101E-4	0.0	0.0	18.111446404138473	0.0
9	1.3387920347229101E-4	0.0	0.0	18.510138672078956	0.0
10	1.3387920347229101E-4	0.0	0.0	22.368001799336493	0.0
11	1.3387920347229101E-4	0.0	0.0	23.1473126427487	0.0
12	1.3387920347229101E-4	0.0	0.0	26.06132738552659	0.0
13	2.6775840694458203E-4	0.0	0.0	26.563909915361567	0.0
14	2.6775840694458203E-4	0.0	0.0	26.897536890414518	0.0
15	2.6775840694458203E-4	0.0	0.0	27.42783241536826	0.0
16	2.6775840694458203E-4	0.0	0.0	27.956253631473395	0.0
17	2.6775840694458203E-4	0.0	0.0	28.511450688272983	0.0
18	2.6775840694458203E-4	0.0	0.0	29.00452779466143	0.0
19	2.6775840694458203E-4	0.0	0.0	29.54539977668949	0.0
20	2.6775840694458203E-4	0.0	0.0	29.87086012033063	0.0
21	2.6775840694458203E-4	0.0	0.0	30.157227736557857	0.0
22	4.0163761041687307E-4	0.0	0.0	30.54869052751084	0.0
23	4.0163761041687307E-4	0.0	0.0	30.855273903462383	0.0
24	4.0163761041687307E-4	0.0	0.0	31.13267161305697	0.0
25	4.0163761041687307E-4	0.0	0.0	31.35638376205917	0.0
26	4.0163761041687307E-4	0.0	0.0	31.58197021990998	0.0
27	4.0163761041687307E-4	0.0	0.0	31.84611388836081	0.0
28	4.0163761041687307E-4	0.0	0.0	32.075850601519264	0.0
29	4.0163761041687307E-4	0.0	0.0	32.309871449188826	0.0
30	4.0163761041687307E-4	0.0	0.0	32.58338666188271	0.0
31	4.0163761041687307E-4	0.0	0.0	32.83307137635854	0.0
32	4.0163761041687307E-4	0.0	0.0	33.07954298995103	0.0
33	4.0163761041687307E-4	0.0	0.0	33.32748727478171	0.0
34	4.0163761041687307E-4	0.0	0.0	33.56579225696239	0.0
35	4.0163761041687307E-4	0.0	0.0	33.833684543110444	0.0
36	4.0163761041687307E-4	0.0	0.0	34.081628827941124	0.0
37	4.0163761041687307E-4	0.0	0.0	34.31712234684888	0.0
38	4.0163761041687307E-4	0.0	0.0	34.54565414717609	0.0
39	4.0163761041687307E-4	0.0	0.0	34.78596731740885	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTGCG	30	2.1644773E-6	45.000004	1
TAGCACG	30	2.1644773E-6	45.000004	1
CGAACCC	35	1.2111559E-7	45.0	34
ACGTTAG	25	3.8894897E-5	45.0	1
CGTTCAT	25	3.8894897E-5	45.0	17
TCGACGG	40	6.8102963E-9	45.0	2
TACGGCT	1770	0.0	43.728817	7
CGTTTTT	6175	0.0	43.433197	1
ACGGCTG	1810	0.0	42.76243	8
CGGCTGT	1870	0.0	41.99198	9
GAATCTG	5750	0.0	41.947823	1
TAGCCGT	200	0.0	41.625	44
TGATACC	2770	0.0	41.34477	4
AATCTGT	5890	0.0	41.256367	2
TCACGAC	60	3.6379788E-12	41.250004	25
GATACCT	2815	0.0	40.52398	5
CGATGAA	690	0.0	40.434784	19
GAATGAC	770	0.0	40.324673	1
CATCGTT	90	0.0	40.0	36
TAACGGG	130	0.0	39.80769	3
>>END_MODULE