SRR3550298_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420479
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5576	1.3261066545534974	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	5146	1.2238423321973273	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	2325	0.5529408127397564	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	2314	0.5503247486794822	No Hit
GAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	1699	0.40406298530961116	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1173	0.27896755842741255	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	669	0.15910425966576214	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	640	0.15220736350685765	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	607	0.14435917132603532	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTC	497	0.11819853072329416	No Hit
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	471	0.11201510658082806	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	429	0.10202649835069051	TruSeq Adapter, Index 22 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1622054E-6	45.000004	9
GGACGAT	20	7.0281775E-4	45.0	8
ACCCGAT	25	3.886572E-5	45.0	41
GCTACGA	20	7.0281775E-4	45.0	10
TTGCACG	20	7.0281775E-4	45.0	1
CCTATCG	20	7.0281775E-4	45.0	40
CGTTTTT	3605	0.0	43.87656	1
ATTGCGG	60	3.6379788E-12	41.250004	2
ACAACGA	110	0.0	40.909092	13
CACAACG	105	0.0	40.714287	12
CGTTATT	250	0.0	40.500004	1
TAAACGG	45	1.9232175E-8	40.0	2
TCGAGAG	45	1.9232175E-8	40.0	1
TACGGCT	360	0.0	38.75	7
AACACGT	215	0.0	38.720932	41
CACGAGC	35	6.238841E-6	38.571426	41
CTAACGG	35	6.238841E-6	38.571426	2
TTGCGCG	35	6.238841E-6	38.571426	14
GCGAGAC	240	0.0	37.500004	21
ATGTACG	30	1.1386907E-4	37.500004	1