##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550298_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420479
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94741235590838	33.0	31.0	34.0	30.0	34.0
2	32.064735694291514	33.0	31.0	34.0	30.0	34.0
3	31.812492419359824	33.0	31.0	34.0	30.0	34.0
4	35.6025128484419	37.0	35.0	37.0	33.0	37.0
5	33.38688495739383	37.0	35.0	37.0	30.0	37.0
6	34.461825679760466	37.0	35.0	37.0	30.0	37.0
7	35.60752142199729	37.0	35.0	37.0	32.0	37.0
8	35.50490274187296	37.0	35.0	37.0	33.0	37.0
9	37.51479384226085	39.0	37.0	39.0	35.0	39.0
10	37.186813134544174	39.0	37.0	39.0	34.0	39.0
11	37.13592593209173	39.0	37.0	39.0	34.0	39.0
12	37.18195439011223	39.0	37.0	39.0	34.0	39.0
13	37.246863695927736	39.0	37.0	39.0	34.0	39.0
14	38.49181290861137	40.0	38.0	41.0	34.0	41.0
15	38.54452659942589	40.0	38.0	41.0	34.0	41.0
16	38.395781953438814	40.0	38.0	41.0	34.0	41.0
17	38.31412983763755	40.0	38.0	41.0	34.0	41.0
18	38.07198219173847	39.0	37.0	41.0	34.0	41.0
19	37.78158718984777	39.0	37.0	41.0	33.0	41.0
20	37.86729896142257	39.0	35.0	41.0	34.0	41.0
21	37.866559328765526	39.0	35.0	41.0	34.0	41.0
22	38.00177654532093	40.0	35.0	41.0	34.0	41.0
23	38.06399606163447	40.0	36.0	41.0	34.0	41.0
24	37.913724585532215	40.0	35.0	41.0	34.0	41.0
25	37.590198321438166	39.0	35.0	41.0	33.0	41.0
26	37.64715954899056	40.0	35.0	41.0	33.0	41.0
27	37.71210452840689	40.0	35.0	41.0	34.0	41.0
28	37.58882132044644	40.0	35.0	41.0	33.0	41.0
29	37.53750841302419	40.0	35.0	41.0	33.0	41.0
30	37.280506279742866	40.0	35.0	41.0	33.0	41.0
31	37.084265801621484	39.0	35.0	41.0	32.0	41.0
32	36.89499356686065	39.0	35.0	41.0	32.0	41.0
33	36.57584326446743	40.0	35.0	41.0	31.0	41.0
34	36.27255820147974	40.0	35.0	41.0	29.0	41.0
35	36.1046615883314	40.0	35.0	41.0	28.0	41.0
36	35.94173787513764	40.0	35.0	41.0	27.0	41.0
37	35.918000661150735	40.0	35.0	41.0	26.0	41.0
38	35.73102818452289	39.0	35.0	41.0	25.0	41.0
39	35.59264790869461	39.0	35.0	41.0	24.0	41.0
40	35.4947785739597	39.0	35.0	41.0	24.0	41.0
41	35.31081457100117	39.0	35.0	41.0	23.0	41.0
42	35.360013223014704	39.0	35.0	41.0	23.0	41.0
43	35.29271378594412	39.0	35.0	41.0	23.0	41.0
44	35.23101510420259	39.0	35.0	41.0	23.0	41.0
45	35.1638845221759	39.0	35.0	41.0	23.0	41.0
46	35.04956490098198	39.0	35.0	41.0	23.0	41.0
47	34.91229288501923	38.0	35.0	40.0	23.0	41.0
48	34.86898751186147	38.0	35.0	40.0	23.0	41.0
49	34.79012269340443	38.0	34.0	40.0	22.0	41.0
50	34.70774521438645	38.0	34.0	40.0	22.0	41.0
51	33.47629964873394	36.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	20.0
10	13.0
11	7.0
12	6.0
13	8.0
14	12.0
15	18.0
16	35.0
17	74.0
18	159.0
19	348.0
20	568.0
21	930.0
22	1423.0
23	2169.0
24	3416.0
25	5492.0
26	7502.0
27	8201.0
28	7628.0
29	7008.0
30	7409.0
31	8481.0
32	10417.0
33	14402.0
34	23653.0
35	29919.0
36	31095.0
37	46801.0
38	79663.0
39	123555.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.772785323404975	17.022966664209154	21.17228208780938	21.031965924576497
2	35.695718454429354	25.44336340221509	23.57787190323417	15.283046240121385
3	28.621167763431703	25.0737848977	31.74070524330585	14.564342095562441
4	24.96866668727808	23.25680949583689	34.910185764330684	16.86433805255435
5	23.593806111601292	31.027708874878414	28.73389634202897	16.644588671491324
6	23.13195189296017	32.992372984144275	30.172731575179736	13.70294354771582
7	73.74018678697391	4.769560429890672	17.462941074346162	4.027311708789262
8	74.90861612589451	5.907548296109913	15.051643482789867	4.132192095205705
9	69.46458681646408	6.351090066329116	16.53067097286666	7.653652144340145
10	34.99865629436904	26.66791920642886	23.73673833889445	14.596686160307648
11	26.482416482154875	23.223514135069763	32.462263275930546	17.831806106844812
12	23.15525864549716	21.261941737875137	35.54184632288414	20.040953293743563
13	21.778257653771057	21.600603121677896	38.35720689975005	18.263932324801
14	19.452814528192846	25.385809992889065	35.66408786170058	19.497287617217506
15	18.737202095705136	24.25709726288352	38.619526777793894	18.386173863617447
16	22.50599911053822	24.48850002021504	33.8780295805498	19.12747128869694
17	21.48716107106419	23.90535555877939	34.56391401235258	20.04356935780384
18	22.346894850872456	23.731030562762946	33.665652743656636	20.256421842707958
19	21.35445527600665	26.044344664061704	31.950228192133256	20.650971867798393
20	23.468948508724573	25.91187669300964	33.06490930581551	17.554265492450277
21	22.613733385020417	26.420820064735693	33.063958009793595	17.9014885404503
22	21.56683211289981	22.499102214379317	33.916557069437474	22.017508603283396
23	21.575155953091592	25.300431174921933	33.713693192763486	19.410719679222982
24	22.16329471864231	24.09276087509721	33.392630785366215	20.351313620894267
25	21.370151660368293	27.02869822274121	31.363992018626373	20.23715809826412
26	20.57296559400113	25.572264013184963	32.46725758004562	21.387512812768293
27	21.98397541851079	24.55580421376573	31.56328853521817	21.89693183250531
28	19.78196295177643	26.326879582571305	32.82827442036344	21.062883045288824
29	21.681225459535437	25.40602503335482	31.534987478566112	21.377762028543636
30	21.873625079968324	24.290630447656124	32.33478960899355	21.500954863382
31	24.07849143476844	23.82164150885062	30.38439494005646	21.715472116324477
32	24.890660413480816	24.560085045864362	30.239322296713983	20.30993224394084
33	22.428944132762872	24.210721581815026	29.81623339096602	23.544100894456086
34	21.913579512888873	25.66810708739319	32.05582205056614	20.362491349151803
35	21.176562919908008	25.879532628264435	31.38325576307021	21.560648688757343
36	21.492868847195698	29.50872695188107	28.85019228070843	20.148211920214802
37	22.63323495346973	26.095952473250744	30.576794560489347	20.694018012790174
38	22.583054088313567	26.29263292578226	28.843771032560483	22.280541953343686
39	23.92485712722871	25.421245769705504	29.6021917860345	21.051705317031292
40	22.01655730726148	25.05547244927809	31.68006012190859	21.24791012155185
41	20.056887502110687	27.482228601190545	30.580361920571537	21.880521976127227
42	21.64745445075735	26.157073242658967	30.96088032933868	21.234591977245
43	22.46984986170534	25.460248906604132	29.393382309223526	22.676518922466997
44	22.25652172879026	24.935609150516434	29.395522725272844	23.41234639542046
45	21.684554995612146	23.748153891157468	29.718012076702998	24.84927903652739
46	23.3771484426095	25.414824521557556	28.71653518962897	22.491491846203974
47	20.871910368888816	24.82169145189177	32.41517412284561	21.891224056373805
48	21.637465842527213	24.84832774050547	30.38011410795783	23.13409230900949
49	21.685268467628585	24.17385886096571	31.983523552900383	22.157349118505323
50	20.72683772554634	23.904404262757474	31.918597599404492	23.450160412291694
51	20.733021149688806	23.47465628485608	30.382492348012626	25.409830217442487
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	147.0
1	295.0
2	443.0
3	5486.0
4	10529.0
5	7265.0
6	4001.0
7	3835.0
8	3669.0
9	3595.0
10	3521.0
11	3385.0
12	3249.0
13	3260.5
14	3272.0
15	2997.5
16	2723.0
17	2601.5
18	2480.0
19	2410.5
20	2341.0
21	2337.0
22	2333.0
23	2385.0
24	2437.0
25	2699.0
26	3362.5
27	3764.0
28	4433.0
29	5102.0
30	5900.5
31	6699.0
32	7855.5
33	9012.0
34	10001.0
35	10990.0
36	11764.5
37	12539.0
38	13793.5
39	15048.0
40	16161.0
41	17274.0
42	18798.5
43	20323.0
44	22699.0
45	25075.0
46	34306.0
47	43537.0
48	38672.5
49	33808.0
50	33407.0
51	33006.0
52	30031.0
53	27056.0
54	24948.0
55	22840.0
56	21265.5
57	19691.0
58	17936.0
59	16181.0
60	15496.0
61	14811.0
62	13296.0
63	11781.0
64	10279.0
65	8777.0
66	7335.0
67	5893.0
68	4978.0
69	4063.0
70	3653.0
71	3243.0
72	2707.0
73	2171.0
74	1804.5
75	1232.0
76	1026.0
77	777.5
78	529.0
79	423.5
80	318.0
81	273.5
82	229.0
83	163.5
84	98.0
85	62.5
86	27.0
87	19.5
88	12.0
89	10.0
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420479.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.48787537317135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24993156502067	33.5874581431484
2	9.602380137732853	8.927885022574817
3	4.799311477860849	6.693293815794779
4	3.24347056397278	6.0312822141806555
5	2.421436732128353	5.628372451360108
6	1.8411876086181858	5.135574005284176
7	1.4099432704208084	4.588168692699492
8	1.1122553989630528	4.13651122961051
9	0.8675588154414077	3.6297869482022698
>10	2.4129752983993877	14.638682757726054
>50	0.021058628429982962	0.6721470894053254
>100	0.01386787725876423	1.519792619560816
>500	0.001540875250973803	0.45748981289529045
>1k	0.0020545003346317376	1.7934269230983961
>5k	0.0010272501673158688	2.560128274458927
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5576	1.3261066545534974	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	5146	1.2238423321973273	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	2325	0.5529408127397564	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	2314	0.5503247486794822	No Hit
GAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCT	1699	0.40406298530961116	No Hit
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1173	0.27896755842741255	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	669	0.15910425966576214	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCT	640	0.15220736350685765	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTT	607	0.14435917132603532	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTC	497	0.11819853072329416	No Hit
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	471	0.11201510658082806	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	429	0.10202649835069051	TruSeq Adapter, Index 22 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.134720164383953E-4	0.0	0.0	0.11700941069589682	0.0
2	7.134720164383953E-4	0.0	0.0	0.5365309563616733	0.0
3	7.134720164383953E-4	0.0	0.0	0.7893378741863446	0.0
4	7.134720164383953E-4	0.0	0.0	1.4702280018740532	0.0
5	9.512960219178603E-4	0.0	0.0	3.3178827004440175	0.0
6	9.512960219178603E-4	0.0	0.0	4.378577764882432	0.0
7	9.512960219178603E-4	0.0	0.0	5.198832759781107	0.0
8	9.512960219178603E-4	0.0	0.0	6.218384271271574	0.0
9	9.512960219178603E-4	0.0	0.0	6.76252559580859	0.0
10	9.512960219178603E-4	0.0	0.0	8.15831468396757	0.0
11	9.512960219178603E-4	0.0	0.0	8.946939086137476	0.0
12	9.512960219178603E-4	0.0	0.0	10.197893354959463	0.0
13	9.512960219178603E-4	0.0	0.0	10.601718516263595	0.0
14	9.512960219178603E-4	0.0	0.0	10.81766271323895	0.0
15	9.512960219178603E-4	0.0	0.0	11.10186239978691	0.0
16	9.512960219178603E-4	0.0	0.0	11.451463687841724	0.0
17	9.512960219178603E-4	0.0	0.0	11.855051025140376	0.0
18	9.512960219178603E-4	0.0	0.0	12.295025435277386	0.0
19	9.512960219178603E-4	0.0	0.0	12.650572323469186	0.0
20	9.512960219178603E-4	0.0	0.0	12.95570052249934	0.0
21	9.512960219178603E-4	0.0	0.0	13.285800242104838	0.0
22	9.512960219178603E-4	0.0	0.0	13.665843002861022	0.0
23	9.512960219178603E-4	0.0	0.0	14.015206466910357	0.0
24	9.512960219178603E-4	0.0	0.0	14.32913415414325	0.0
25	9.512960219178603E-4	0.0	0.0	14.62332244892135	0.0
26	9.512960219178603E-4	0.0	0.0	14.911089495551503	0.0
27	9.512960219178603E-4	0.0	0.0	15.206466910356998	0.0
28	9.512960219178603E-4	0.0	0.0	15.510168165354274	0.0
29	0.0011891200273973254	0.0	0.0	15.813155948335114	0.0
30	0.0011891200273973254	0.0	0.0	16.216029813617325	0.0
31	0.0011891200273973254	0.0	0.0	16.532335740905015	0.0
32	0.0011891200273973254	0.0	0.0	16.864813700565307	0.0
33	0.0011891200273973254	0.0	0.0	17.186113931968066	0.0
34	0.0011891200273973254	0.0	0.0	17.506938515359863	0.0
35	0.0011891200273973254	0.0	0.0	17.818725786543443	0.0
36	0.0011891200273973254	0.0	0.0	18.147398562116063	0.0
37	0.0011891200273973254	0.0	0.0	18.470363561557175	0.0
38	0.0011891200273973254	0.0	0.0	18.841131186099663	0.0
39	0.0011891200273973254	0.0	0.0	19.195488954264064	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1622054E-6	45.000004	9
GGACGAT	20	7.0281775E-4	45.0	8
ACCCGAT	25	3.886572E-5	45.0	41
GCTACGA	20	7.0281775E-4	45.0	10
TTGCACG	20	7.0281775E-4	45.0	1
CCTATCG	20	7.0281775E-4	45.0	40
CGTTTTT	3605	0.0	43.87656	1
ATTGCGG	60	3.6379788E-12	41.250004	2
ACAACGA	110	0.0	40.909092	13
CACAACG	105	0.0	40.714287	12
CGTTATT	250	0.0	40.500004	1
TAAACGG	45	1.9232175E-8	40.0	2
TCGAGAG	45	1.9232175E-8	40.0	1
TACGGCT	360	0.0	38.75	7
AACACGT	215	0.0	38.720932	41
CACGAGC	35	6.238841E-6	38.571426	41
CTAACGG	35	6.238841E-6	38.571426	2
TTGCGCG	35	6.238841E-6	38.571426	14
GCGAGAC	240	0.0	37.500004	21
ATGTACG	30	1.1386907E-4	37.500004	1
>>END_MODULE