FastQCFastQC Report
Sat 18 Jun 2016
SRR3550295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292929
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173345.917474882992124No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.742159362849018No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC10860.37073830177278455No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG10000.3413796517244793No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC9830.33557619764516317No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC8170.2789071754588996TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC8120.2772002772002772TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT4240.14474497233117922Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3730.12733461009323077No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA3270.11163114611390473No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3120.10651045133803755No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA3000.1024138955173438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGGT302.1599444E-645.0000044
GTATGAG302.1599444E-645.0000041
TCATCCC302.1599444E-645.00000414
TATAAGG600.045.0000042
CGGAATG207.024671E-445.06
CTAGACG253.8836653E-545.026
AGCCGTT253.8836653E-545.045
CCCTACT253.8836653E-545.018
GGACTAG207.024671E-445.01
ACGGGAG502.1827873E-1145.05
GCCGATT207.024671E-445.09
GTAGCCG253.8836653E-545.043
CGCTAGA253.8836653E-545.024
GCTAGAC253.8836653E-545.025
GCTACGG207.024671E-445.02
CGTTATT14250.044.684211
CGTTTTT98900.044.4312441
GTTATTT14800.043.0236472
GCGATAC501.0750227E-940.59
GCCTTCG451.9199433E-839.99999623
Produced by FastQC (version 0.11.5)