##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550295_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	292929
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.934868176247488	33.0	31.0	34.0	30.0	34.0
2	32.09692109692109	33.0	31.0	34.0	30.0	34.0
3	31.745583400755816	33.0	31.0	34.0	30.0	34.0
4	35.70893629514319	37.0	35.0	37.0	33.0	37.0
5	31.352450593829904	37.0	35.0	37.0	0.0	37.0
6	33.408641684503756	37.0	35.0	37.0	17.0	37.0
7	35.334913921120815	37.0	35.0	37.0	32.0	37.0
8	35.58560265456817	37.0	35.0	37.0	33.0	37.0
9	37.66082566082566	39.0	37.0	39.0	35.0	39.0
10	37.4158755193238	39.0	37.0	39.0	34.0	39.0
11	37.39471680850991	39.0	37.0	39.0	34.0	39.0
12	37.38474852267956	39.0	37.0	39.0	35.0	39.0
13	37.23670923670924	39.0	37.0	39.0	34.0	39.0
14	38.48278593106179	40.0	38.0	41.0	33.0	41.0
15	38.591074970385314	40.0	38.0	41.0	34.0	41.0
16	38.64291005670316	40.0	38.0	41.0	34.0	41.0
17	38.56644442851339	40.0	38.0	41.0	34.0	41.0
18	38.06818375783893	39.0	38.0	40.0	34.0	41.0
19	37.438744542192815	37.0	37.0	40.0	34.0	41.0
20	36.71447688689068	36.0	35.0	40.0	34.0	41.0
21	36.46347067036722	36.0	35.0	40.0	33.0	41.0
22	36.38823059512715	35.0	35.0	40.0	33.0	41.0
23	36.33827992448682	35.0	35.0	40.0	33.0	41.0
24	36.14756818205094	35.0	35.0	40.0	33.0	41.0
25	35.95603030085789	35.0	35.0	40.0	33.0	41.0
26	35.97596345872208	35.0	35.0	40.0	33.0	41.0
27	35.904263490470385	35.0	35.0	40.0	33.0	41.0
28	35.715163059990644	35.0	35.0	40.0	32.0	41.0
29	35.522973143662796	35.0	35.0	40.0	31.0	41.0
30	35.03926207374483	35.0	35.0	40.0	30.0	41.0
31	34.50169153617429	35.0	35.0	40.0	24.0	41.0
32	33.4917539745126	35.0	33.0	40.0	18.0	41.0
33	32.30575327127051	35.0	32.0	40.0	12.0	41.0
34	31.25021080193494	35.0	29.0	40.0	8.0	41.0
35	30.55899211071625	35.0	23.0	40.0	7.0	41.0
36	30.12248019144571	35.0	22.0	40.0	7.0	41.0
37	29.889594406835787	35.0	21.0	40.0	7.0	41.0
38	29.66759180552284	35.0	21.0	40.0	7.0	41.0
39	29.498967326553533	35.0	19.0	40.0	7.0	41.0
40	29.31418193487159	35.0	18.0	40.0	7.0	41.0
41	29.11207152586463	35.0	18.0	40.0	7.0	41.0
42	28.99048233530992	35.0	18.0	40.0	7.0	41.0
43	28.83175445244411	35.0	18.0	40.0	7.0	41.0
44	28.65635358738807	35.0	18.0	40.0	7.0	41.0
45	28.62728511004373	35.0	18.0	40.0	7.0	41.0
46	28.581734823114132	35.0	18.0	39.0	7.0	41.0
47	28.572797503831985	35.0	18.0	39.0	7.0	41.0
48	28.57338809062947	35.0	18.0	39.0	7.0	41.0
49	28.587992312130243	35.0	18.0	39.0	7.0	41.0
50	28.503203848031433	35.0	18.0	39.0	7.0	41.0
51	27.899064278374624	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	10.0
10	7.0
11	9.0
12	7.0
13	8.0
14	11.0
15	27.0
16	46.0
17	116.0
18	239.0
19	455.0
20	872.0
21	1394.0
22	2229.0
23	3436.0
24	5903.0
25	10517.0
26	16480.0
27	18777.0
28	16915.0
29	12802.0
30	10456.0
31	9406.0
32	9540.0
33	11386.0
34	16470.0
35	22651.0
36	21327.0
37	24360.0
38	35129.0
39	41921.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.37738154979534	10.760286622355588	13.083716531992396	49.778615295856675
2	58.37626182453769	15.927067651205581	14.817925162752749	10.87874536150398
3	19.80240945758187	16.694147728630487	53.13301175370141	10.370431060086233
4	17.590952073710696	15.751939889870926	54.580461477013195	12.07664655940518
5	14.422266146404079	28.53865612486302	46.40373605890847	10.63534166982443
6	16.165692027760993	22.048004806625496	52.712432022776845	9.07387114283666
7	46.95745385400558	4.493580355649321	45.06313816658644	3.4858276237586585
8	46.54370171611551	5.338494993667407	43.83587831863694	4.281924971580144
9	42.7475599889393	5.308112204663929	45.55916280054211	6.3851650058546605
10	25.782015437187848	13.800955180265525	49.997439652612066	10.419589729934557
11	20.572220572220573	14.539017987293848	52.372759269311	12.516002171174584
12	17.384758764069108	13.560282525799765	55.7657316278006	13.289227082330532
13	15.01626674040467	15.82636065394686	57.1548054306675	12.00256717498097
14	12.53614357062633	18.400363227949434	55.94666284321457	13.116830358209668
15	11.504494263114953	15.494198252818942	59.98279446555309	13.01851301851302
16	13.105223450051037	15.087615087615088	56.91106035933622	14.896101102997655
17	12.751895510516201	15.797343383550281	55.218158666434526	16.23260243949899
18	13.560965285103215	15.21597383666349	56.40274605791847	14.82031482031482
19	14.464597223217911	15.68844327465017	55.16729309832758	14.679666403804333
20	15.325215325215325	15.878591740660704	55.45678304298994	13.33940989113403
21	14.790956170266515	17.35505873436908	55.090824056341305	12.763161039023107
22	13.550723895551481	15.313267037404968	54.7979203151617	16.338088751881855
23	13.484154863465209	16.479761307347516	54.496140703037256	15.539943126150021
24	13.900979418220796	15.755695066039893	55.53939691870726	14.803928597032046
25	13.000078517319896	17.181979250944767	54.50535795363381	15.312584278101518
26	11.857822202649789	16.99387906284458	55.864390347148976	15.283908387356663
27	13.916682882200124	16.75218226942365	54.802016870982385	14.52911797739384
28	12.477767650181443	16.22031277203691	56.12076646559405	15.181153112187594
29	13.185447668206288	15.71575364678813	55.636348739797015	15.462449945208565
30	13.714244748727507	16.85664444285134	54.5790959584063	14.850014850014851
31	16.14725752656787	16.93823417961349	51.748034506655195	15.166473787163442
32	16.23123692089209	18.809677430367085	50.16027774648464	14.798807902256177
33	15.51058447610172	18.537597847942674	48.12292398499295	17.82889369096266
34	15.616753547788031	20.199092612885718	47.044847044847046	17.139306794479207
35	16.48488200212338	21.050493464286568	43.718102338791994	18.746522194798057
36	18.33277005690799	21.48063182545941	42.630808148049525	17.555789969583074
37	17.620652103410723	23.516961447995932	41.89786603579707	16.964520412796276
38	17.695414247138388	24.46497274083481	39.81818119749154	18.021431814535262
39	17.337306992479405	23.715644405299578	41.1242997449894	17.822748857231616
40	18.5225771432668	22.746808953705504	41.41515520825866	17.31545869476904
41	16.15476787890581	23.192650778857676	39.78677426953289	20.865807072703625
42	18.232063059649267	23.750465129775474	38.53630060526612	19.481171205309135
43	18.30170450860106	22.99089540468851	38.225645122196845	20.481754964513584
44	16.84981684981685	24.344124344124342	39.29382205244274	19.512236753616065
45	16.86654445275135	23.283799145868112	39.30918413677034	20.540472264610194
46	18.40480116342185	22.83625042245732	40.7672166292856	17.99173178483523
47	16.55179241386138	22.083166910753118	42.87182218216701	18.493218493218492
48	16.844013395737534	21.819280439970097	41.46704491532078	19.869661248971592
49	16.87405480508929	20.249616801340938	43.31698124801573	19.559347145554042
50	16.226457605767948	20.845324293600157	42.79876693669797	20.129451163933922
51	16.105609209057484	20.99587272001065	41.034175516934134	21.864342553997727
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	290.5
2	406.0
3	16802.5
4	33199.0
5	22697.5
6	12196.0
7	11695.5
8	11195.0
9	10971.0
10	10747.0
11	10252.5
12	9758.0
13	9109.0
14	8460.0
15	7577.5
16	6695.0
17	6046.5
18	5398.0
19	4949.0
20	4500.0
21	4067.5
22	3635.0
23	3437.0
24	3239.0
25	3161.0
26	2977.0
27	2871.0
28	2866.5
29	2862.0
30	3086.5
31	3311.0
32	3619.0
33	3927.0
34	4152.0
35	4377.0
36	4724.0
37	5071.0
38	5266.0
39	5461.0
40	6484.0
41	7507.0
42	8713.5
43	9920.0
44	11589.5
45	13259.0
46	16754.0
47	20249.0
48	19600.5
49	18952.0
50	18318.5
51	17685.0
52	14964.5
53	12244.0
54	10879.0
55	9514.0
56	9172.0
57	8830.0
58	7912.5
59	6995.0
60	6830.5
61	6666.0
62	5965.5
63	5265.0
64	4805.5
65	4346.0
66	3739.5
67	3133.0
68	2847.0
69	2561.0
70	2099.5
71	1638.0
72	1483.5
73	1329.0
74	1212.5
75	850.0
76	604.0
77	460.5
78	317.0
79	231.5
80	146.0
81	103.0
82	60.0
83	38.0
84	16.0
85	15.0
86	14.0
87	11.5
88	9.0
89	7.0
90	5.0
91	3.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	292929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.458029670442855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07773595317066	39.773253641015174
2	7.458520577657166	7.22850422899439
3	2.7542379175462166	4.003948281837399
4	1.4666445897342646	2.842828281813499
5	0.9177514535964328	2.2236213584234
6	0.7379226192379464	2.1454965706513986
7	0.5604248038775343	1.9009957242044775
8	0.4627208212603159	1.7938031428611259
9	0.4457072069280205	1.9438283751883407
>10	3.0265148876173287	23.55883141313391
>50	0.06144821335032104	1.990924645297193
>100	0.02542684690358112	2.309226161564408
>500	0.0028252052115090133	1.2362420180184737
>1k	0.0014126026057545067	1.1157666054098074
>5k	0.0	0.0
>10k+	7.063013028772533E-4	5.932729551586994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17334	5.917474882992124	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2174	0.742159362849018	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGC	1086	0.37073830177278455	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCG	1000	0.3413796517244793	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	983	0.33557619764516317	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	817	0.2789071754588996	TruSeq Adapter, Index 27 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	812	0.2772002772002772	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	424	0.14474497233117922	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	373	0.12733461009323077	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA	327	0.11163114611390473	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	312	0.10651045133803755	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	300	0.1024138955173438	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.827593034489586E-4	0.0	0.0	0.14952428745532192	0.0
2	6.827593034489586E-4	0.0	0.0	0.8595939630422389	0.0
3	6.827593034489586E-4	0.0	0.0	1.1446459722321791	0.0
4	6.827593034489586E-4	0.0	0.0	1.5208463484325554	0.0
5	6.827593034489586E-4	0.0	0.0	2.6470578194716126	0.0
6	6.827593034489586E-4	0.0	0.0	3.2345722000894415	0.0
7	6.827593034489586E-4	0.0	0.0	3.8654417964762793	0.0
8	6.827593034489586E-4	0.0	0.0	4.75029785374613	0.0
9	6.827593034489586E-4	0.0	0.0	5.089970607211987	0.0
10	6.827593034489586E-4	0.0	0.0	6.142102693826832	0.0
11	6.827593034489586E-4	0.0	0.0	7.045734631941529	0.0
12	6.827593034489586E-4	0.0	0.0	8.26445998859792	0.0
13	6.827593034489586E-4	0.0	0.0	8.587063759477553	0.0
14	6.827593034489586E-4	0.0	0.0	8.724981138774242	0.0
15	6.827593034489586E-4	0.0	0.0	9.013105564829702	0.0
16	6.827593034489586E-4	0.0	0.0	9.535757811619881	0.0
17	6.827593034489586E-4	0.0	0.0	10.108592867213558	0.0
18	6.827593034489586E-4	0.0	0.0	10.817979783497025	0.0
19	6.827593034489586E-4	0.0	0.0	11.281914730190593	0.0
20	6.827593034489586E-4	0.0	0.0	11.660846143604765	0.0
21	6.827593034489586E-4	0.0	0.0	12.078353457663802	0.0
22	6.827593034489586E-4	0.0	0.0	12.499274568240086	0.0
23	6.827593034489586E-4	0.0	0.0	12.898347381106001	0.0
24	6.827593034489586E-4	0.0	0.0	13.225730467109777	0.0
25	6.827593034489586E-4	0.0	0.0	13.518975587941105	0.0
26	6.827593034489586E-4	0.0	0.0	13.796517244793106	0.0
27	6.827593034489586E-4	0.0	0.0	14.067572688262343	0.0
28	6.827593034489586E-4	0.0	0.0	14.35160055849711	0.0
29	6.827593034489586E-4	0.0	0.0	14.658159485745692	0.0
30	6.827593034489586E-4	0.0	0.0	15.006025350852937	0.0
31	6.827593034489586E-4	0.0	0.0	15.320777389742908	0.0
32	6.827593034489586E-4	0.0	0.0	15.609584575101817	0.0
33	6.827593034489586E-4	0.0	0.0	15.88951588951589	0.0
34	6.827593034489586E-4	0.0	0.0	16.18241963069549	0.0
35	6.827593034489586E-4	0.0	0.0	16.497513049237188	0.0
36	6.827593034489586E-4	0.0	0.0	16.77744436365126	0.0
37	6.827593034489586E-4	0.0	0.0	17.088099846720535	0.0
38	6.827593034489586E-4	0.0	0.0	17.36120356810012	0.0
39	6.827593034489586E-4	0.0	0.0	17.67083491221422	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGT	30	2.1599444E-6	45.000004	4
GTATGAG	30	2.1599444E-6	45.000004	1
TCATCCC	30	2.1599444E-6	45.000004	14
TATAAGG	60	0.0	45.000004	2
CGGAATG	20	7.024671E-4	45.0	6
CTAGACG	25	3.8836653E-5	45.0	26
AGCCGTT	25	3.8836653E-5	45.0	45
CCCTACT	25	3.8836653E-5	45.0	18
GGACTAG	20	7.024671E-4	45.0	1
ACGGGAG	50	2.1827873E-11	45.0	5
GCCGATT	20	7.024671E-4	45.0	9
GTAGCCG	25	3.8836653E-5	45.0	43
CGCTAGA	25	3.8836653E-5	45.0	24
GCTAGAC	25	3.8836653E-5	45.0	25
GCTACGG	20	7.024671E-4	45.0	2
CGTTATT	1425	0.0	44.68421	1
CGTTTTT	9890	0.0	44.431244	1
GTTATTT	1480	0.0	43.023647	2
GCGATAC	50	1.0750227E-9	40.5	9
GCCTTCG	45	1.9199433E-8	39.999996	23
>>END_MODULE